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Studies on Bovine Brain Membrane-Bound Neuraminidase (Sialidase)

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Enzymes of Lipid Metabolism

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 101))

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Abstract

Mammalian neuraminidases have been localised inside the lysosomes, in the cytosol as well as in the various membranes of the cell cytocavitary system (1). Brain tissue is especially rich in membrane-bound neuraminidase which reaches its highest specific activity in the plasma membranes isolated from synaptosomes (2, 3). This enzyme cleaves terminal N-acylneuraminic acid residues, predominantly from oligosialogangliosides, thereby degrading them to monosialogangliosides (4–5).

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Abbreviations

Des-GM1 :

(monosialo-gangliotetraitol): Galßl→3GalNAcßl→4Ga1[3←2αNeuAc]ß1→4[3H]sorbitol

Des-GD1a :

(disialo-gangliotetraitol): Gal [3←2αNeuAc] ß1→3GalNAcß 1→4Gal (3←2αNeuAc)ß1→4[3H]sorbitol

GA2 :

Gangliotriaosylceramide (GgOse3Cer): Ga1NACß1→4Galß1→4Glc1→l’ceramide

GM1 :

(ganglioside II3 NeuAc GgOse4-Cer): Galßl→3GaiNACß1→4Ga1[3←2αNeuAc]ß1→4G1cß1→1’ceramide

GM2 :

(ganglioside II3 NeuAc-GgOse3-Cer): Ga1NAcß1→4Gal [3←2αNeuAc]ß1→4Glcß1→1’ceramide

GDIa :

(ganglioside IV3 NeuAc, II3 NeuAc-GgOse4-Cer): Gal [3←2αNeuAc]ß1→3GalNAcß1→4Gal [3←2αNeuAc]ß1→4Glcß1→1’ceramide

NeuAc:

N-Acetylneuraminic acid

Sialyl-lactitol::

NeuAcα2→3Ga1ß1→4[3H]sorbitol

References

  1. Symposion on Neuraminidase (1974) Behring Institute, Mitteilungen 55.

    Google Scholar 

  2. C.L. Schengrund and A. Rosenberg (1970) J. Biol. Chem. 245, 6196–6200.

    PubMed  CAS  Google Scholar 

  3. G. Tettamanti, I.G. Morgan, G. Gambos, G. Vincendon and P. Mandel (1972) Brain Res. 47, 515–518.

    Article  PubMed  CAS  Google Scholar 

  4. Z. Leibowitz and S. Gatt (1968) Biochim. Biophys. Acta 152, 136–143.

    Article  Google Scholar 

  5. R. Öhman, A. Rosenberg and L. Svennerholm (1970) Biochemistry 9, 3774–3782.

    Article  PubMed  Google Scholar 

  6. L. Warren (1959) J. Biol. Chem. 234, 1971–1975.

    PubMed  CAS  Google Scholar 

  7. J. Schraven, C. Cap, G. Nowoczek and K. Sandhoff (1977) Analyt. Biochem. 78, 333–339.

    Article  PubMed  CAS  Google Scholar 

  8. K. Sandhoff, J. Schraven and G. Nowoczek (1976) FEBS Lett. 62, 284–287.

    Article  PubMed  CAS  Google Scholar 

  9. V.P. Bhavanandan, A.K. Yeh and R. Carubelli (1975) Anal. Biochem. 69, 385–394.

    Article  PubMed  CAS  Google Scholar 

  10. M. Shinitzky and Y. Barenholz (1974) J. Biol. Chem. 249, 2652.

    PubMed  CAS  Google Scholar 

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Author information

Author notes

  1. W. Ziegler

    Present address: CNRS, F-67085, Strasbourg Cedex, France

Authors and Affiliations

  1. Max-Planck-Institut für Psychiatrie, Kraepelinstr. 2, D-8000, München 40, W.-Germany

    K. Sandhoff & B. Pallmann

  2. Institut für Physiologische Chemie I, Philipps-Universität, D-3550, Marburg, W.-Germany

    H. Wiegandt & W. Ziegler

Authors
  1. K. Sandhoff
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  2. B. Pallmann
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  3. H. Wiegandt
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  4. W. Ziegler
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, The Hebrew University, Hadassah Medical School, P.O. Box 1172, Jerusalem, Israel

    Shimon Gatt

  2. C.N.R.S. Center for Neurochemistry, Louis Pasteur University, 11 rue Humann, 67085, Strasbourg Cedex, France

    Louis Freysz  & Paul Mandel  & 

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© 1978 Plenum Press, New York

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Sandhoff, K., Pallmann, B., Wiegandt, H., Ziegler, W. (1978). Studies on Bovine Brain Membrane-Bound Neuraminidase (Sialidase). In: Gatt, S., Freysz, L., Mandel, P. (eds) Enzymes of Lipid Metabolism. Advances in Experimental Medicine and Biology, vol 101. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9071-2_42

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  • DOI: https://doi.org/10.1007/978-1-4615-9071-2_42

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9073-6

  • Online ISBN: 978-1-4615-9071-2

  • eBook Packages: Springer Book Archive

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