Abstract
The purpose of this paper is to illustrate experimental strategies that may be adopted in the laboratory to approach transport studies in isolated enterocytes with isotopic methods. Since the work carried out with this biological preparation is vast I shall focus on methodologies set up to: a) investigate the energetics of organic solute active transport (e.g.sugars and dipeptides), b) reveal the separate pathways involved in the transfer of a given solute across the cell membrane (e.g. K+ transport) and c) study intracellular transport events.
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References
Ahnen, D.J., Reed, T.A. and Bozdech, J.M. (1988). Isolation and characterization of populations of mature and immature rat colonocytes. American Journal of Physiology 254, G610 - G621.
Bolufer, J., Santos, F.J. and Vila, A. (1982). Interactions between monosaccharides and Leucine in basolateral membrane of isolated chick intestinal cells. Revista de Fi iolo.ía 38, 65–70.
Bronner, F., Pansu, D., Bosshard, A. and Lipton, J.H. (1983). Calcium uptake by isolated rat intestinal cells Journal of Cell. Physiology 116, 322–328.
Brown, P.D. and Sepúlveda, F.V. (1985a). A rabbit jejunal isolated enterocyte preparation suitable for transport studies. Journal of Physiology 363, 257–270.
Brown, P.D. and Sepúlveda, F.V. (1985b). Potassium movements associated with amino acid and sugar transport in enterocytes isolated from rabbit jejunum. Journal of Physiology 363, 271–285.
Burton, K.A., Ilundâin, A., O’Brien, J.A. and Sepúlveda, F.V. (1986). Calcium mobilisation by inositol-1,4,5-triphosphate in chicken enterocytes. Journal of Physiology 378, 77 p.
Calonge, M.L., Ilundain, A. and Bolufer, J. (1989). Ionic dependence of glycylsarcosine uptake by isolated enterocytes. Journal of Cell Phvsiologv 138, 579–585.
Carter-Su, C. and Kimmich, G.A. (1979). Membrane potentials and sugar transport by ATP-depleted intestinal cells: effect of anion gradients. American Journal of Physiology 237, C67 - C74.
Carter-Su, C. and Kimmich, G.A. (1980). Effect of membrane potential on Na+-dependent sugar transport by ATP-depleted intestinal cells. American Journal of Phvsiologv 238, C73 - C80.
Crane, R.K. (1968). Absorption of sugars. In “Handbook of Physiology”, C.F. Code, ed., sect. 6, vol III, pp 1323 Americal Journal Society, Washington D.C.
Eddy, A.A. (1987). The sodium gradient hypothesis of organic solute transport with special reference to amino acids. In “Amino acid transport in animal cells”, ed.Yudilevich,D.L. and Boyd C.A.R., pp 47–86. Manchester University Press.
Ferrer, A., Planas, J.M. and Moretó, M. (1986). Preparation and properties of isolated epithelial cells from chicken caecum and jejunum. Revista espanola de Fisiologia 42, 341–348.
Geck, P. and Heinz, E. (1986). The Na-K-Cl cotransport system. Journal of Membrane Bioloqy 91, 97–105.
Girardi, A.J., Mc Michael, H. and Henle, W. (1956). The use of HeLa cells in suspension for the quantitative study of virus propagation. Virology 2, 532–544.
Ilundâin, A., O’Brien, J.A., Burton, K.A. and Sepúlveda, F.V. (1987). Inositol triphosphate and calcium mobilisation in permeabilised enterocytes. Biochimica et biophysica acta 896, 113–116.
Kaunitz, J.D. (1988). Preparation and charaterization of viable epithelial cells from rabbit distal colon. American Journal of Ph siolo 254, G502 - G512.
Kimmich, G.A. (1975). Preparation and characterisation of isolated intestinal epithelial cells and their use in studying intestinal transport. In: “Methods in memr ban biology”, Korth, E.,ed., vol IV. Plenum Press, New York, pp 51–115.
Kimmich, G.A. (1981). Intestinal absorption of sugars. In “Physiology of the Gastrointestinal Tract”, chapter 41, Johnson L.R., ed., Raven Press, New York, pp. 1035–1061.
Kimmich, G.A., Carter-Su, C. and Randles, J. (1977). Energetics of Nat-dependent sugar transport by isolated intestinal cells: evidence for a major role for membrane potential. American Journal of Physiology 233, E357 - E362.
Kimmich, G.A., Randles, J., Restrepo, D. and Montrose, M. (1985). The potential dependence of the intestinal Nat-dependent sugar transporter.Annual New York Academiin 456, 63–76.
Kimmich, G.A. and Randles, J. (1988). Nat-coupled sugar transport: membrane potential-dependent Km and Ki for Nat. American Journal of PhysiolomL 255, C486 - C494.
Lazdunski, M. (1983). Apamin a neurotoxin specific for one class of Ca2+-dependent K+ channels. Cell Calcium 4, 421–428.
Latorre, R., Oberhauser, A., Labarca, P. and Alvarez, 0. (1989). Varieties of calcium-activated potassium channels. Annual Review of Physiology 51, 385–399
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.
Montero, M.C., Bolufer,J. and Ilundâin, A. (1988). Potassium transport in epithelial cells isolated from small intestine of the chicken. Pflügers Archiv European Journal of Physiology 412, 422–426.
Montero, M.C. and Ilundâin, A. (1989). Effects of anisosmotic buffers on K+ transport in isolated chicken enterocytes. Biochimica et Biophysica Acta 979, 269–271.
Morris, A.P., Gallacher, D.U. and Lee, J.A.C. (1986) A large conductance, voltage-and calcium-activated K+ channel in the basolateral membrane of rat enterocytes. FEBS Letters 206, 87–92.
Schwarz, W. and Passow, H. (1983). Ca2+-activated K+-channels in erythrocytes and excitable cells. Annual Review of Physiology 45, 359–374.
Sepúlveda, F.V. and Mason, W.T (1985) Single channel recordings obtained from basolateral membranes of isolated rabbit enterocytes. FEBS Letters 191, 87–91.
Sepúlveda, F.V. and Smith, S.M. (1987). Calcium transport by permeabilised rabbit small intestinal epithelial cells. Pflücers Archiv European Journal of Physioloav 408, 231–238.
Sheppard, D.N., Giraldez, F. and Sepúlveda, F.V. (1988). Kinetics of voltage-and Ca2+ activation and Ba2+ blockade of a large-conductance K+ channel from Necturus enterocytes. Journal of Membrane Biology 105, 65–75.
Velasco, G., Dominguez, P., Shears,S.B. and Lazo, P.S. (1986a). Permeability properties of isolated enterocytes from rat small intestine. Biochimica et Biophysica Acta 889, 361–365.
Velasco, G., Shears, S.B., Michell, R.H. and Lazo, P.S. (1986b). Calcium uptake by intracellular compartments in permeabilised enterocytes. Effect of inositol 1,4,5 triphosphate. Biochemical and Biophysical Research Communications 139, 612–618.
Verbost, P.M., Senden, M.H.M.N. and Van Os, C.H. (1987). Nanomolar concentrations of Cd2+ inhibit Ca2+ transport systems in plasma membranes and intracellular Ca2+ stores in intestinal epithelium. Biochimica et. Biophysica Acta 902, 247–252.
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Ilundáin, A. (1991). Transport Studies in Isolated Enterocytes. In: Yudilevich, D.L., Devés, R., Perán, S., Cabantchik, Z.I. (eds) Cell Membrane Transport. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9601-8_7
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DOI: https://doi.org/10.1007/978-1-4757-9601-8_7
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