Skip to main content
SpringerLink
Log in

New Approach to the Study of Transport of Biomolecules by Microcarrier Cell Cultures Perfused in a Column Applying a High Resolution Paired-Tracer Technique

  • Chapter
Cell Membrane Transport

Abstract

The functional and structural asymmetry of the plasma membrane makes the net uptake of any substrate the result of an interaction between two vectorial components of different magnitudes: one directed to the inside and another one directed to the outside of the cell (Stein, 1986). At the same time, the physiological activity of the cell determines membrane transport not as an isolated process with a finality in itself, but as one step of the metabolic pathways to which is coupled and from which is in many instances dependent. Hence, the mechanisms regulating influx and efflux must be directional. On the one hand, to meet the cellular requirements in response to internal stimuli and, on the other hand, to preserve the functional integrity of the organism responding to extracellular humoral and/or tissue (paracrine) stimuli (Shotwell et al., 1983).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bashor, M.M. 1979. Dispersion and disruption of tissues. Method. Enzymol., 58: 119–131.

    Article  CAS  Google Scholar 

  • Boyd, C.A.R. and Parsons, D.S. 1979. Movements of monosaccharides between blood and tissue of vascularly perfused small intestine. J. Physiol, 287: 371–391.

    PubMed  CAS  Google Scholar 

  • Boyd, C.A.R. and Yudilevich, D.L. 1987. Blood-tissue movement of amino acids in various organs: the basolateral membrane and its importance in the polarity of transepithelial transport. In: “Amino acid transport in animal cells”. D.L. Yudilevich and C.A.R. Boyd eds., Manchester University Press, Manchester. Bravo, I.R. and Yudilevich, D.L. 1971. Liver distribution and uptake of molecules studied by rapid indicator dilution technique. Am. J. Physiol., 221: 1449–1455.

    Google Scholar 

  • Bustamante, J.C., Mann, G.E. and Yudilevich, D.L. 1981. Specificity of neutral amino acid uptake at the basolateral side of the epithelium in the cat salivary gland “in situ”. J. Physiol., 313: 65–79.

    PubMed  CAS  Google Scholar 

  • Calandra, R.S. and Nicola, A.F. 1985. “Endocrinología Molecular”, 2 ed. El Ateneo, Buenos Aires.

    Google Scholar 

  • Christensen, H.N. 1975. “Biological Transport”, 2d. ed., W.A. Benjamin Inc., Reading, Massachussetts.

    Google Scholar 

  • Clayman, S. and Scholefield, T. 1969. The uptake of amino acids by mouse pancreas in vitrro. IV. The role of exchange diffusión. Biochim. Biophys. Acta, 173: 277–289.

    Article  CAS  Google Scholar 

  • Dorr, R.T. and Fritz, W.L. 1980. “Cancer Chemotherapy Handbook”, Elsevier, New York.

    Google Scholar 

  • Dowling, M.D., Krakoff, I.H. and Karnofsky, D.A. 1970. Mechanism of action of anticancer drugs. In: “Chemotherapy of Cancer”, W.H. Cole ed., Lea and Febiger, Philadelphia.

    Google Scholar 

  • Duval, D., Durant, S. and Delarche, U. 1983. Non-genomic effects of steroids. Interactions of steroids molecules with membrane structures and functions. Biochim. Biophys. Acta, 737: 409–442.

    Article  PubMed  CAS  Google Scholar 

  • Franchi-Gazzola, R., Gazzola, G.C., Dall’Asta, V. and Guidotti, G.G. 1982. The transport of alanine serine and cysteine in cultured human fibroblasts. J. Biol. Chem., 257: 9582–9587.

    PubMed  CAS  Google Scholar 

  • Gazzola, G.C., Dall’Asta, V. and Guidotti, G.G. 1980. The transport of neutral amino acids in cultured human fibroblasts. J. Biol. Chem., 255: 929–936.

    PubMed  CAS  Google Scholar 

  • Griffiths, B. 1986. Scaling-up of animal cultures. In: “Animal cell culture..A practical approach”, R.I. Freshney ed., IRL Press Ltd., Oxford.

    Google Scholar 

  • Guidotti, G.G., Borguetti, A.F. and Gazzola, G.C. 1978. The regulation of amino acid transport in animal cells. Biochim. Biophys. Acta, 515: 239–266.

    Article  Google Scholar 

  • Jensen, E.V., Suzuki, T., Kawashima, T. Stumpf, W.E., Jumblut, P.W. and Desombre, E.R. 1967. A two-step mechanism for the interaction of estradiol with rat uterus. Biochemistry, 59: 632–638.

    Google Scholar 

  • King, W. and Greene, H. 1984. Monoclonal antibodies localize oestrogen receptor in the nuclei of target cells. Nature, 307: 745–747.

    Article  PubMed  CAS  Google Scholar 

  • Kovacevic, Z. and Morris, H.P. 1972. The role of glutamine in the oxidative metabolism of malignant cells. Cancer Res., 32: 326–333.

    PubMed  CAS  Google Scholar 

  • Mann, G.E., Habara, Y and Peran, S. 1986a. Characteristics of L-glutamine transport in the perfused rat exocrine pancreas. Lack of sensitivity to insulin and streptozotocin-induced experimental diabetes. Pancreas, 3: 239–235.

    Article  Google Scholar 

  • Mann, G.E., Munoz, M. and Peran, S. 1986b. Fasting and refeeding modulates neutral amino acid transport activity in the basolateral membrane of the rat exocrine pancreatic epithelium. Fasting-induced insulin insensitivity. Biochim. Biophys. Acta, 862: 119–126.

    Article  PubMed  CAS  Google Scholar 

  • Mann, G.E. and Perân, S. 1986. Basolateral amino acid transport systems in the perfused exocrine pancreas. Sodium-dependency and kinetic interaction between influx and efflux mechanisms. Biochim. Biophys. Acta, 858: 263–274.

    Article  PubMed  CAS  Google Scholar 

  • Mann, G.E., Norman, P.S.R. and Smith, I.C.H. 1989a. Amino acid efflux in the isolated perfused rat pancreas:trans-stimulation by extracellular amino acids. J. Physiol., 415: 485–502.

    Google Scholar 

  • Mann, G.E., Pearson, J.D., Sheriff, C-J. Toothill. V.J. 1989b. Expression of amino acid transport systems in cultured human umbilical vein endothelial cells. J. Physiol., 410: 325–339.

    PubMed  CAS  Google Scholar 

  • Martin de Julian, P. and Yudilevich, D.L. 1964. A theory for the quantification of transcapillary exchange by tracer dilution curves. Am. J. Physiol., 207: 162–168.

    Google Scholar 

  • Milgrom, E., Atger, S. and Baulieu, T. 1973. Studies on estrogen entry into uterine cells and on estradiol receptor complex attachment to the nucleus. Is the entry of estrogen into uterine cells a protein-mediated process?. Biochim. Biophys. Acta, 320: 267–283.

    Article  PubMed  CAS  Google Scholar 

  • Müller, R.E., Traish, M. and Wotiz, H. 1977. Interaction of estrogen-receptor complex (R-E) with uterine nuclei. Proc. Natl. Acad. Sci. USA, 43: 3464–3468.

    Google Scholar 

  • Müller, R.E. and Wotiz, H. 1979. Kinetics of estradiol entry into uterine cells. Endocrinology, 105: 1107–1114.

    Article  PubMed  Google Scholar 

  • Müller, R.E., Traish, M. and Wotiz, H. 1985. Interaction of E2 and estriol with uterine estrogen receptor in vivo and in excised uterior cell suspensions at 37 C: non-cooperative estradiol binding and absence of estriol inhibition of estradiol-induced receptor activation and transformation. Endocrinology, 117: 1839–1847.

    Article  PubMed  Google Scholar 

  • Munoz, M., Emery, P.W., Peran, S. and Mann, G.E. 1988. Dietary regulation of amino acid transport activity in the exocrine pancreas. Biochim. Biophys. Acta, 945: 273–280.

    Article  PubMed  CAS  Google Scholar 

  • Neame, K.D. and Richards, T.G. 1972. “Elementary kinetics of membrane carrier transport”, Blackwell Scientific Plublications, Oxford.

    Google Scholar 

  • Peran, S. and Peran-McGee, M. 1985. Transport of L-phenylalanine by isolated VERO cells immobilized in a microcarrier perfusion column: application of a rapid paired-tracer dilution technique. J. Physiol. 360: 71 P.

    Google Scholar 

  • Peran, S. and McGee, M.P. 1986. Unidirectional flux of phenylalanine into Vero cells. Measurements using paired tracers in perfused cultures. Biochim. Biophys. Acta, 856: 23 1236.

    Google Scholar 

  • Peran, S., Munoz, M. and Saiz, M.T. 1990. Rapid and steady-state amino acid transport in perfused human fibroblasts and colon adenocarcinoma cells. Effects of methotrexate. Biochim. Biophys. Acta, 1024:233–240..

    Google Scholar 

  • Pietras, R.J. and Szego, C.M. 1977. Specific binding sites for estrogen at the outer surfaces of isolated endometrial cells. Nature, 265: 69–72.

    Article  PubMed  CAS  Google Scholar 

  • Pietras, R.J. and Szego, C.M. 1979a. Estrogen receptors in uterine plasma membrane. J. Steroid. Biochem., 11: 71–83.

    Article  Google Scholar 

  • Pietras, R.J. and Szego, C.M. 1979b. Metabolic and proliferative responses to estrogen by hepatocytes selected for plasma membrane binding sites specific for estradiol-1713. J. Cell. Physiol., 98: 145–160.

    Article  PubMed  CAS  Google Scholar 

  • Pietras, R.J. and Szego, C.M. 1984. Specific internalization of estrogen and binding to nuclear matrix in isolated uterine cells. Biochem. Biophys. Res. Commun., 123: 84–91.

    Article  PubMed  CAS  Google Scholar 

  • Rao, G., Schulze-Hagen, K., Rao, M. and Brener, H. 1976. Kinetics of steroid transport through cell membranes: comparison of the uptake of cortisol by isolated rat liver cells with binding of cortisol to rat liver cytosol. J. Steroid Biochem., 7: 1123–1129.

    Article  PubMed  CAS  Google Scholar 

  • Rao, M., Rao, G., Eckel, J. and Brener, H. 1977a. Factors involved in the uptake of corticosterone by rat liver cells. Biochim. Biophys. Acta, 500: 322–332.

    Article  PubMed  CAS  Google Scholar 

  • Rao, M., Rao, G. and Brener, H. 1977b. Uptake of estrone, estradiol-17B, and testosterone by isolated rat liver cells. Biochem. Biophys. Res. Commun., 77: 566–573.

    Article  PubMed  CAS  Google Scholar 

  • Rao, G. 1981. Mode of entry of steroid and thyroid hormones into the cells. Mol. Cell. Endocrinol., 21: 97–108.

    Article  PubMed  CAS  Google Scholar 

  • Sauer, L.A., Webster-tayman, J. and Dauchy, R.T. 1982. Amino acid, glucose and lactic acid utilization in vivo by rats tumors. Cancer Res., 42: 4090–4097.

    PubMed  CAS  Google Scholar 

  • Shotwell, M.A., Kilberg, M.S. and Oxender, D.L. 1983. The regulation of neutral amino acid transport in mammalian cells. Biochim. Biophys. Acta, 737: 267–284.

    Article  PubMed  CAS  Google Scholar 

  • Sly, W.S. and Grubb, J. 1979. Isolation of fibroblasts from patients. Method Enzymol., 58: 439–443.

    Article  Google Scholar 

  • Smith, M.A. and Vale, W.W. 1980. Superfusion of rat anterior pituitary cells attached to Cytodex Beads: Validation of a technique. Endocrinology, 102: 1425–1431.

    Article  Google Scholar 

  • Snell, K. 1984. Enzymes of serine metabolism in normal, developing and neoplastic rat tissues. Adv. Enzyme. Regul., 22: 325–400.

    Article  PubMed  CAS  Google Scholar 

  • Snell, K. 1986. Enzymes of serine metabolism in normal and neoplastic rat tissues. Biochim. Biophys. Acta, 843:276–281. Stein, W.D. 1986. “Transport and diffusion across cell membranes”, Academic Press Inc., London.

    Google Scholar 

  • Szego, C.M. and Pietras, R.J. 1984. Subcellular distribution of oestrogen receptors. Nature, 317: 88–89.

    Article  Google Scholar 

  • Thorndike, J., Pellinemi, F.T. and Beck, W.S. 1979. Serine hydroxymethyltransferase activity and serine incorporation in leukocytes. Cancer Res., 39: 3435–3440.

    PubMed  CAS  Google Scholar 

  • Van Wezel, A.L. 1967. Growth of cell strains and primary cells on microcarriers in homogeneous cultures. Nature, 216: 64–65.

    Article  PubMed  Google Scholar 

  • Weber, G. 1983. Biochemical strategy of cancer cells and the design of chemotherapy. Cancer Res., 43: 3466–3492.

    PubMed  CAS  Google Scholar 

  • Welshons, W.J., Lieberman, M.A., Gorski, J. 1984. Nuclear localization of unoccupied oestrogen receptors. Nature, 307: 747–749.

    Article  PubMed  CAS  Google Scholar 

  • Yudilevich, D.L. 1970. Serial barriers to blood-tissue transport studied by single injection indicator diffusion technique. In: “Capillary Permeability”, Alfred Benzon Symposium II, C. Crone ed., Munksgaard, Copenhagen.

    Google Scholar 

  • Yudilevich, D.L. and Mann, G.E. 1982. Unidirectional uptakes of substrates at the blood side of secretory epithelia: stomach, salivary gland and pancreas. Fed. Proc., 41: 3045–3053.

    PubMed  CAS  Google Scholar 

  • Yudilevich, D.L. and Swery, J.H. 1985. Transport of amino acids in the placenta. Biochim. Biophys. Acta, 822: 169–201.

    Article  PubMed  CAS  Google Scholar 

  • Zielke, H.R., Zielke, C.L. and Ozand, P.T. 1984. Glutamine: a major energy source for cultured mammalian cells. Fed. Proc., 43: 121–125.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Membrane Transport Group, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Málaga, Málaga, Spain

    S. Perán, M. Muñoz & M. T. Saiz

Authors
  1. S. Perán
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Muñoz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. T. Saiz
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of London, London, UK

    David L. Yudilevich

  2. University of Chile, Santiago, Chile

    Rosa Devés

  3. University of Málaga, Málaga, Spain

    Salvador Perán

  4. The Hebrew University of Jerusalem, Jerusalem, Israel

    Z. Ioav Cabantchik

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer Science+Business Media New York

About this chapter

Cite this chapter

Perán, S., Muñoz, M., Saiz, M.T. (1991). New Approach to the Study of Transport of Biomolecules by Microcarrier Cell Cultures Perfused in a Column Applying a High Resolution Paired-Tracer Technique. In: Yudilevich, D.L., Devés, R., Perán, S., Cabantchik, Z.I. (eds) Cell Membrane Transport. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9601-8_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-9601-8_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9603-2

  • Online ISBN: 978-1-4757-9601-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation