Abstract
Large fragment knock-in mouse models such as reporters and conditional mutant mice are important models for biological research. Here we describe 2-cell (2C)-homologous recombination (HR)-CRISPR, a highly efficient method to generate large fragment knock-in mouse models by CRISPR-based genome engineering. Using this method, knock-in founders can be generated routinely in a time frame of about two months with high germline transmission efficiency. 2C-HR-CRISPR will significantly promote the advancement of basic and translational research using genetic mouse models.
Dr. Posfai as Co-Corresponding Author
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Acknowledgements
This work was supported by CIHR (FDN-143334) and Genome Canada and Ontario Genomics (OGI-099). The authors wish to thank Dr. Janet Rossant for her guidance and support during the development of 2C-HR-CRISPR and critical discussion and comments.
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Gu, B., Gertsenstein, M., Posfai, E. (2020). Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos. In: Larson, M. (eds) Transgenic Mouse. Methods in Molecular Biology, vol 2066. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9837-1_7
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DOI: https://doi.org/10.1007/978-1-4939-9837-1_7
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