Abstract
Cell enumeration using the hemocytometer is applicable when determining the number of cells in a suspension, and when the number of samples to be analyzed is relatively small. Hemocytometry is also useful for determining the proportion of singly dispersed cells in a suspension, and for estimating the frequency of viable cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Further Reading
Bainbridge, D. R. and Macey, M. M. (1983), Hoechst 33258: a fluorescent nuclear counterstain suitable for double-labelling immunofluorescence. J. Immunol. Methods 62, 193–195.
Baserga, R. (1989), Measuring parameters of growth, in Cell Growth and Division, Baserga, R., ed., IRL, New York.
Branch, D. R. and Guilbert, L. J. (1986), Practical in vitro assay systems for the measurement of hematopoietic growth factors J. Tissue Culture Methods 10, 101–108.
Hamilton, L. H. (1956), Errors in blood cell counting. 1. Technical errors. 2. Statistical errors. Can. J. Med. Tech. 18, 8–14.
Hanks, J. H. and Wallace, R. E. (1958), Determination of cell viability. Proc. Soc. Exp. Biol Med. 98, 188–192.
Harris, M. (1959), Growth measurements on monolayer cultures with an electronic cell counter. Can. Res. 19, 1020–1024.
Jones, K. H. and Senft, J. A. (1985), An improved method to determine cell viability by simultaneous staining with fluorescein diacetate propidium iodide. J. Hist. Cytol. 33, 77–79.
Kaltenbach, J. P., Kaltenbach, M. H., and Lyons, W. B. (1958), Nigrosin as a dye for differentiating live and dead ascites cells. Exp. Cell Res. 15, 112–117.
Latt, S. A. and Stetten, G. (1976), Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J. Histochem. Cytochem. 24, 24–33.
Phillips, H. J. and Terryberry, J. E. (1957), Counting actively metabolizing tissue cultured cells. Exp. Cell Res. 13, 341–347.
Schrek, R. (1944), Studies in vitro on the physiology of normal and of cancerous cells. II. The survival and the glycolysis of cells under anaerobic conditions. Arch. Pathol. 37, 319–327.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Richardson, A., Fedoroff, S. (2001). Quantification of Cells in Culture. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-207-4:333
Download citation
DOI: https://doi.org/10.1385/1-59259-207-4:333
Publisher Name: Humana Press
Print ISBN: 978-0-89603-902-5
Online ISBN: 978-1-59259-207-4
eBook Packages: Springer Book Archive