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Effects of Danggui-Shaoyao-San on depression- and anxiety-like behaviors of rats induced by experimental tooth movement

Effekte von Danggui-Shaoyao-San auf durch experimentelle Zahnbewegungen induziertes depressions- und angstähnliches Verhalten von Ratten

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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie Aims and scope Submit manuscript

Abstract

Purpose

To investigate the effects of Danggui-Shaoyao-San (DSS) on depression- and anxiety-like behavior induced by experimental tooth movement (ETM) in rats.

Materials and methods

Thirty-six rats were randomly divided into a sham group (n = 12; rats underwent all operation procedures, except placement of orthodontic forces, and received saline treatment), ETM group (n = 12; rats received saline treatment and ETM), and DETM group (n = 12; rats received DSS [dose: 150 mg/kg twice daily from preoperative day 5 to postoperative day 7] treatment and ETM). The vacuous chewing movement (VCM) test, open-field test, and elevated plus maze test were performed to assess the depression- and anxiety-like behaviors of the rats.

Results

DSS pretreatment significantly decreased the ETM-induced VCM time (P < 0.05, DETM vs. ETM), increased the ETM-induced time to the central area of experimental device during the 5 min open-field test (P < 0.05, DETM vs. ETM), and increased the ratio of time spent in the open arms of the 5 min elevated plus maze test induced by ETM (P < 0.01, DETM vs. ETM).

Conclusions

DSS pretreatment can restore the impaired abilities of rats caused by ETM-induced depression- and anxiety-like behavior.

Zusammenfassung

Zielsetzung

Untersucht werden sollten die Wirkungen von Danggui-Shaoyao-San (DSS) auf depressions- und angstähnliches Verhalten bei Ratten, das durch experimentelle Zahnbewegungen (ETM) induziert wurde.

Materialien und Methoden

Die Ratten wurden randomisiert in eine Sham- (n = 12; die Ratten wurden allen chirurgischen Eingriffen mit Ausnahme der Platzierung der kieferorthopädischen Kräfte unterzogen und erhielten eine Kochsalzlösung), eine ETM- (n = 12; die Ratten erhielten eine Kochsalzlösung und ETM) und eine DETM-Gruppe (n = 12; die Ratten erhielten DSS [Dosis: 150 mg/kg 2‑mal täglich] vom präoperativen Tag 5 bis zum postoperativen Tag 7 und ETM) eingeteilt. Der VCM(„vacuous chewing movement“)-, der Open-field- und der Elevated-plus-Labyrinth-Test wurden durchgeführt, um das depressions- und angstähnliche Verhalten der Ratten zu beurteilen.

Ergebnisse

Die DSS-Vorbehandlung verringerte signifikant die ETM-induzierte VCM-Zeit (p < 0,05, DETM vs. ETM), erhöhte die ETM-induzierte Zeit zum zentralen Bereich des Versuchsgeräts während des 5‑minütigen Open-field-Tests (p < 0,05, DETM vs. ETM) und erhöhte den Anteil der Zeit, die in den offenen Armen des 5‑minütigen Elevated-plus-Labyrinth-Tests verbracht wurde, induziert durch ETM (p < 0,01, DETM vs. ETM).

Schlussfolgerungen

Eine DSS-Vorbehandlung kann die beeinträchtigten Fähigkeiten der Ratten, die durch ETM-induzierte Depression und angstähnliches Verhalten verursacht werden, wiederherstellen.

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Acknowledgements

This work was supported by the Project of Clinical Application Research and Achievements of Capital Characteristics (Z181100001718021) and (Z171100000417033).

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Authors and Affiliations

  1. Institute of Stomatology, The Medical Center of Air Force of PLA, Beijing 100142, China

    Hongshi Li, Dongxia Li, Guizhi Zhao, Yuan Gao & Jie Ke

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Correspondence to Jie Ke.

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H. Li, D. Li, G. Zhao, Y. Gao and J. Ke declare that they have no competing interests.

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All experimental procedures were performed in accordance with the ethical guidelines of the Animal Use and Care Committee for Research and Education of Institute of Stomatology, Chinese PLA General Hospital (Beijing, China). The Animal Care and Use Committee of the Chinese PLA General Hospital approved this study (permit number: 20130169).

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Li, H., Li, D., Zhao, G. et al. Effects of Danggui-Shaoyao-San on depression- and anxiety-like behaviors of rats induced by experimental tooth movement. J Orofac Orthop 83, 23–33 (2022). https://doi.org/10.1007/s00056-021-00323-0

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