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Usefulness of interferon-gamma release assays for diagnosing TB infection and problems with these assays

  • Review Article
  • Published:
Journal of Infection and Chemotherapy

Abstract

The specificity of the tuberculin skin test (TST) in the diagnosis of tuberculosis infection is seriously compromised because of extensive use of the bacille Calmette-Guérin (BCG) vaccination. The interferon-gamma release assay (IGRA), a new diagnostic using Mycobacterium tuberculosis-specific antigens has been introduced in response to these needs. In this review, published findings on the performance of the QuantiFERON-TB (QFT), one of the IGRA formats, are summarized and discussed. In addition to its high specificity, the QFT has considerably high sensitivity, comparable with or superior to that of the TST, if applied to patients with active tuberculosis as a surrogate of latent tuberculosis infection. When applied to patients with immunosuppression, such as aging patients, or those with HIV infection, those with immunosuppressive drug therapies, or those with renal hemodialysis, QFT is shown to be more robust than the TST. As regards the dynamics of QFT responses to chemotherapy, there are many reports showing a decrease in responses during the treatment, which indicates the possibility that QFT could be used as a tool for monitoring the progress of treatment. However, there are discordant reports that warrant further study.

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References

  1. Pai M, O’Brien R. Review of T-cell-based assays for the diagnosis of latent tuberculosis infection. Ann Intern Med 2008;149:1–12.

    Google Scholar 

  2. MetaDiSc software, version 1.4: Hospital Ramon y Cajal, Madrid Spain

  3. Katiyar SK, Sampath A, Bihari S, Mamtani M, et al. Use of the QuantiFERON-TB Gold In-Tube test to monitor treatment efficacy in active pulmonary tuberculosis. Int J Tuber Lung Dis 2008;2:1146–1152.

    Google Scholar 

  4. Mori T, Sakatani M, Yamagishi F, Takashima T, Kawabe Y, Nagao K, et al. Specific detection of tuberculosis infection: an interferon-gamma-based assay using new antigens. Am J Respir Crit Care Med 2004;170:59–64.

    Article  PubMed  Google Scholar 

  5. Kang YA, Lee HW, Hwang SS, Um SW, Han SK, Shim YS, et al. Usefulness of whole-blood interferon-gamma assay and interferon-gamma enzyme linked immunospot assay in the diagnosis of active pulmonary tuberculosis. Chest 2007;132:959–965.

    Article  PubMed  Google Scholar 

  6. Kobashi Y, Obase Y, Fukuda M, Yoshida K, Miyashita N, Oka M. Clinical reevaluation of the QuantiFERON TB-2G test as a diagnostic method for differentiating active tuberculosis from nontuberculous mycobacteriosis. Clin Infect Dis 2006;43:1540–1546.

    Article  PubMed  Google Scholar 

  7. Ravn P, Munk ME, Andersen AB, Lundgren B, Lundgren JD, Nielsen LN, et al. Prospective evaluation of a whole-blood test using Mycobacterium tuberculosis-specific antigens ESAT-6 and CFP-10 for diagnosis of active tuberculosis. Clin Diagn Lab Immunol 2005;12:491–496.

    PubMed  CAS  Google Scholar 

  8. Kobashi Y, Mouri K, Yagi S, Obase Y, Miyashita N, Okimoto N, et al. Clinical utility of the QuantiFERON TB-2G test for elderly patients with active tuberculosis. Chest. 2008. DOI 10.1378/chest.07-1995.

  9. Goletti D, Carrara S, Vincenti D, Saltini C, Rizzi EB, Schinina V, et al. Accuracy of an immune diagnostic assay based on RD1 selected epitopes for active tuberculosis in a clinical setting: a pilot study. Clin Microbiol Infect 2006;12:544–550.

    Article  PubMed  CAS  Google Scholar 

  10. Harada N, Higuchi K, Yoshiyama T, Kawabe Y, Fujita A, Sasaki Y, et al. Comparison of the sensitivity and specificity of two whole blood interferon-gamma assays for M. tuberculosis infection. J Infect 2008;56:348–353.

    Article  PubMed  Google Scholar 

  11. Bartu V, Havelkova M, Kopecka E. QuantiFERON®-TB Gold in the diagnosis of active tuberculosis. J Int Med Res 2008;36:434–437.

    PubMed  CAS  Google Scholar 

  12. Kobashi Y, Mouri K, Yagi S, Obase Y, et al. Clinical evaluation for diagnosing active TB disease and transitional change of two commercial blood tests. Scand J InfectDis 2008;40:629–634.

    Article  CAS  Google Scholar 

  13. Kobashi Y, Mouri K, Yagi S, Obase Y, Fukuda M, Miyashita N, et al. Usefulness of the QuantiFERON TB-2G test for the differential diagnosis of pulmonary tuberculosis. Intern Med 2008;47:237–243.

    Article  PubMed  Google Scholar 

  14. Soysal A, Torun T, Efe S, Gencer H, Tahaoglu K, Bakir M. Evaluation of cut-off values of interferon-gamma-based assays in the diagnosis of M. tuberculosis infection. Int J Tuberc Lung Dis 2008;12:50–56.

    PubMed  CAS  Google Scholar 

  15. Nishimura T, Hasegawa N, Mori M, Takebayashi T, Harada N, Higuchi K, et al. Accuracy of an interferon-gamma release assay to detect active pulmonary and extra-pulmonary tuberculosis. Int J Tuberc Lung Dis 2008;12:269–274.

    PubMed  CAS  Google Scholar 

  16. Bua A, Molicotti P, Delogu G, Pirina P, Mura MS, Madeddu G, et al. QuantiFERON TB Gold: a new method for latent tuberculosis infection. New Microbiol 2007;30:477–480.

    PubMed  Google Scholar 

  17. Kang YA, Lee HW, Yoon HI, Cho B, Han SK, Shim YS, et al. Discrepancy between the tuberculin skin test and the whole-blood interferon gamma assay for the diagnosis of latent tuberculosis infection in an intermediate tuberculosis-burden country. JAMA 2005;293:2756–2761.

    Article  PubMed  CAS  Google Scholar 

  18. Aoki M, Aman K, Mitarai S, et al. Sequential analysis of QuantiFERON-TB Gold test results in active tuberculosis patients: changes in values over time and timing of negative conversion Kekkaku 2006;81:277 (in Japanese).

    Google Scholar 

  19. Pai M, Joshi R, Bandyopadhyay M, Narang P, et al. Sensitivity of a whole-blood interferon-gamma assay among patients with pulmonary tuberculosis and variations in T-cell responses during anti-tuberculosis treatment. Infection 2007;35:98–103, DOI 10.10.1007/s15010-007-6114-z

    Article  PubMed  CAS  Google Scholar 

  20. Ferrara G, Losi M, D’Amico R, Roversi P, Piro R, Meacci M, et al. Use in routine clinical practice of two commercial blood tests for diagnosis of infection with Mycobacterium tuberculosis: a prospective study. Lancet 2006;367:1328–1334.

    Article  PubMed  CAS  Google Scholar 

  21. Lee JY, Choi HJ, Park IN, Hong SB, Oh YM, Lim CM, et al. Comparison of two commercial interferon-gamma assays for diagnosing Mycobacterium tuberculosis infection. Eur Respir J 2006;28:24–30.

    Article  PubMed  CAS  Google Scholar 

  22. Mazurek GH, Weis SE, Moonan PK, Daley CL, Bernardo J, Lardizabal AA, et al. Prospective comparison of the tuberculin skin test and two whole-blood interferon-gamma release assays in persons with suspected tuberculosis. Clin Infect Dis 2007;45:837–845.

    Article  PubMed  CAS  Google Scholar 

  23. Dewan PK, Grinsdale J, Kawamura LM. Low sensitivity of a whole-blood interferon-gamma release assay for detection of active tuberculosis. Clin Infect Dis 2007;44:69–73.

    Article  PubMed  Google Scholar 

  24. Palazzo R, Spensieri F, Massari M, Fedele G, Frasca L, Carrara S, et al. Use of whole-blood samples in in-house bulk and single-cell antigen-specific gamma interferon assays for surveillance of Mycobacterium tuberculosis infections. Clin Vaccine Immunol 2008;15:327–337.

    Article  PubMed  CAS  Google Scholar 

  25. Dominguez J, Ruiz-Manzano J, De Souza-Galvao M, Latorre I, Mila C, Blanco S, et al. Comparison of two commercially available gamma interferon blood tests for immunodiagnosis of tuberculosis. Clin Vaccine Immunol 2008;15:168–171.

    Article  PubMed  CAS  Google Scholar 

  26. Pai M, Joshi R, Bandyopadhyay M, Narang P, Dogra S, Taksande B, et al. Sensitivity of a whole-blood interferon-gamma assay among patients with pulmonary tuberculosis and variations in T-cell responses during anti-tuberculosis treatment. Infection 2007;35:98–103.

    Article  PubMed  CAS  Google Scholar 

  27. Tsiouris SJ, Coetzee D, Toro PL, Austin J, Stein Z, El-Sadr W. Sensitivity analysis and potential uses of a novel gamma interferon release assay for diagnosis of tuberculosis. J Clin Microbiol 2006;44:2844–2850.

    Article  PubMed  CAS  Google Scholar 

  28. Adetifa IM, Lugos MD, Hammond A, Jeffries D, Donkor S, Adegbola RA, et al. Comparison of two interferon gamma release assays in the diagnosis of Mycobacterium tuberculosis infection and disease in The Gambia. BMC Infect Dis 2007;7:122.

    Article  PubMed  Google Scholar 

  29. Brock I, Munk ME, Kok-Jensen A, Andersen P. Performance of whole blood IFN-gamma test for tuberculosis diagnosis based on PPD or the specific antigens ESAT-6 and CFP-10. Int J Tuberc Lung Dis 2001;5:462–467.

    PubMed  CAS  Google Scholar 

  30. Taggart EW, Hill HR, Ruegner RG, Litwin CM. Evaluation of an in vitro assay for interferon gamma production in response to the Mycobacterium tuberculosis-synthesized peptide antigens ESAT-6 and CFP-10 and the PPD skin test. Am J Clin Pathol 2006;125:467–473.

    PubMed  Google Scholar 

  31. Mazurek GH, Zajdowicz MJ, Hankinson AL, Costigan DJ, Toney SR, Rothel JS, et al. Detection of Mycobacterium tuberculosis infection in United States Navy recruits using the tuberculin skin test or whole-blood interferon gamma release assays. Clin Infect Dis 2007;45:826–836.

    Article  PubMed  CAS  Google Scholar 

  32. Franken WP, Timmermans JF, Prins C, Slootman EJ, Dreverman J, Bruins H, et al. Comparison of Mantoux and QuantiFERON TB Gold tests for diagnosis of latent tuberculosis infection in Army personnel. Clin Vaccine Immunol 2007;14:477–480.

    Article  PubMed  CAS  Google Scholar 

  33. Soborg B, Andersen AB, Larsen HK, Weldingh K, Andersen P, Kofoed K, et al. Detecting a low prevalence of latent tuberculosis among health care workers in Denmark detected by M. tuberculosis specific IFN-gamma whole-blood test. Scand J Infect Dis 2007;39:554–559.

    Article  PubMed  Google Scholar 

  34. Song KH, Jeon JH, Park WB, Kim SH, et al. Usefulness of the whole-blood interferon-gamma release assay for diagnosis of extrapulmonary tuberculosis. Diagn Microbiol Infect Dis 2009;63:182–187.

    Article  PubMed  CAS  Google Scholar 

  35. Ariga H, Kawabe Y, Nagai H, Kurashima A, et al. Diagnosis of active tuberculous serositis by antigen-specific interferon-γ response of cavity fluid cells. Clin Infect Dis 2007:45:1559–1567.

    Article  PubMed  CAS  Google Scholar 

  36. Diel R, Loddenkemper R, Meywald-Walter K, Gottschalk R, Nienhaus A. Comparative performance Tuberculin Skin Test, Quantiferon-Gold-in Tube Assay, T-Spot.TB in contact investigations for tuberculosis. Chest. 2008 DOI 10.1378/chest.08-2048.

  37. Kipfer B, Reichmuth M, Buechler M, Meisels C, Bodmer T. Tuberculosis in a Swiss army training camp: contact investigation using an interferon gamma release assay. Swiss Med Wkly 2008;138:267–272.

    PubMed  Google Scholar 

  38. Funayama K, Tsujimoto A, Mori M, Yamamoto H, et al. Usefulness of QuantiFERON-TB-2G in contact investigation of a tuberculosis outbreak in a university. Kekkaku 2005;80:527–534.

    PubMed  Google Scholar 

  39. Fukazawa K. Application and problems of QuantiFERON®TB-2G for tuberculosis control programs. 1. Tuberculosis outbreak in a cram school. Kekkaku 2007;82:53–59.

    PubMed  Google Scholar 

  40. Diel R, Loddenkemper R, Meywald-Walter K, Niemann S, Nienhaus A. Predictive value of a whole blood IFN-gamma assay for the development of active tuberculosis disease after recent infection with Mycobacterium tuberculosis. Am J Respir Crit Care Med 2008;177:1164–1170.

    Article  PubMed  Google Scholar 

  41. Higuchi K, Harada N, Fukazawa K, Mori T. Relationship between whole-blood interferon-gamma responses and the risk of active tuberculosis. Tuberculosis (Edinb) 2008;88:244–248.

    Article  CAS  Google Scholar 

  42. Andersen P, Doherty TM, Pai M, Weldingh K. The prognosis of latent tuberculosis: can disease be predicted? Trends Mol Med 2007;13:175–182.

    Article  PubMed  Google Scholar 

  43. Mazurek G, Jereb J, LoBue P, et al. Guidelines for using the QuantiFERON-TB Gold test for detecting Mycobacterium tuberculosis infection: United States. MMWR Recomm Rep 2005;54:49–55.

    PubMed  Google Scholar 

  44. National Institute for Health and Clinical Excellence. Tuberculosis: clinical diagnosis and management of tuberculosis, and measures for its prevention and control. Clinical Guideline 33, National Collaborating Centre for Chronic Conditions, London March 2006.

  45. Canadian Tuberculosis Committee (CTC): Updated recommendations on interferon gamma release assays for latent tuberculosis infection. An Advisory Committee Statement (ACS). Can Commun Dis Rep 2008;34:1–13.

    Google Scholar 

  46. Drobniewski F, Cobelens F, Zellweger JP. Use of gamma-interferon assays in low- and medium-prevalence countries in Europe: a consensus statement of a Wolfheze Workshop organised by KNCV/EuroTB, Vilnius Sept 2006. Euro Surveill 2007;12:E070726.2.

  47. Haute authorite de Sante. Test de detection de la production d O’interferon γ pour de diagnostic de infections tuberculeuses. 2006;1–50, Paris.

  48. Lungenliga Schweiz: Handbuch Tuberkulose. 2007. Bern, pp 19–26.

  49. Committee of TB Prevention. Japanese Society of Tuberculosis. Recommendations for use of QuantiFERON Gold in the diagnosis of tuberculosis infection. Kekkaku 2006;81:393–397.

    Google Scholar 

  50. Connell TG, Curtis N, Ranganathan SC, et al. Performance of a whole blood interferon gamma assay for detecting latent infection with Mycobacterium tuberculosis in children. Thorax 2006;61:616–620.

    Article  PubMed  CAS  Google Scholar 

  51. Lighter J, Rigaud M, Eduardo M, Peng CH, Pollack H. Latent tuberculosis diagnosis in children by using the QuantiFERONTB Gold In-Tube Test. Pediatrics 2009;123:30–37. DOI: 10.1542/peds.2007-3618.

    Article  PubMed  Google Scholar 

  52. Molicotti P, Bua A, Mela G, Olmeo P, et al. Performance of QuantiFERON-TB Testing in a tuberculosis outbreak at a primary school. J Pediatr 2008;152:585–586.

    Article  PubMed  Google Scholar 

  53. Detjen AK, Keil T, Roll S, Hauer B, Mauch H, Wahn U, et al. Interferon gamma release assays improve the diagnosis of tuberculosis and nontuberculous mycobacterial disease in children in a country with a low incidence of tuberculosis. Clin Infect Dis 2007;45:322–328.

    Article  PubMed  CAS  Google Scholar 

  54. Connell TG, Ritz N, Paxton GA, Buttery JP, Curtis N, et al. A three-way comparison of tuberculin skin testing, QuantiFERONTB Gold and T-SPOT. TB in children. PLoS One 2008;3:e2624.

    Article  PubMed  Google Scholar 

  55. Russo C. Agreement between classic diagnostic procedures and the new interferon-gamma release assay for detection of TB disease in children. In: Rethinking the epidemiology of tuberculosis infection. Vancouver, February 21, 22, 2007.

  56. Herrmann JL, Belloy M, Porcher R, Simonney N, et al. Temporal dynamics of interferon gamma responses in children evaluated for tuberculosis. PLoS One. 2009;4:e4130. Epub 2009 Jan 6.

    Article  PubMed  Google Scholar 

  57. Takamatsu I, Study Group of QFT in Pediatrics. Multicenter study of QuantiFERON in child tuberculosis. In: Kato PI, editor. A study of Mycobacterium tuberculosis: 2007 Report of Emerging and Re-emerging Infectious Disease Research Program. Tokyo: Ministry of Health, Labour and Welfare; 2008.

    Google Scholar 

  58. Dogra S, Narang P, Mendiratta DK, et al. Comparison of a whole blood interferon-γ assay with tuberculin skin testing for the detection of tuberculosis infection in hospitalized children in rural India. J Infect 2007;54:267–276.

    Article  PubMed  Google Scholar 

  59. Okada K, Mao TE, Mori T, Miura T, et al. Performance of an interferon-gamma release assay for diagnosing latent tuberculosis infection in children. Epidemiol Infect 2008;136:1179–1187.

    Article  PubMed  CAS  Google Scholar 

  60. Lewinsohn DA, Zalwango S, Stein CM, Mayanja-Kizza H, Okwera A, et al. Whole blood interferon-gamma responses to Mycobacterium tuberculosis antigens in young household contacts of persons with tuberculosis in Uganda. PLoS One 2008;3:e3407. Epub 2008 Oct 15.

    Article  PubMed  Google Scholar 

  61. Connell T, Bar-Zeev N, Curtis N. Early detection of perinatal tuberculosis using a whole blood interferon-γ release assay. Clin Infect Dis 2006;42:482–485.

    Article  Google Scholar 

  62. Tsiouris SJ, Austin J, Toro P, Coetzee D, Weyer K, Stein Z, El-Sadr WM. Results of a tuberculosis-specific IFN-gamma assay in children at high risk of tuberculosis infection. Int J Tuberc Lung Dis 2006;10:939–941.

    PubMed  CAS  Google Scholar 

  63. Nakaoka H, Lawson L, Squire SB, et al. Risk for tuberculosis among children. Emerg Infect Dis 2006;12:1383–1388.

    PubMed  Google Scholar 

  64. Chun JK, Kim CK, Kim HS, Jung GY, et al. The role of a whole blood interferon γ assay for the detection of latent tuberculosis infection in bacille Calmette-Guérin vaccinated children. Diagn Microbiol Infect Dis doi:10.1016/j.diagmicrobio.2008.08.022.

  65. Leyten EMS, Arend SM, Prins C, Cobelens FGJ, et al. Discrepancy between Mycobacterium tuberculosis-specific gamma interferon release assays using short and prolonged in vitro incubation. Clin Vacc Immunol 2007;14:880–885, doi:10.1128/CVI.00132-07.

    Article  CAS  Google Scholar 

  66. Lewinsohn D, Gennaro M, Scholvinck D. Tuberculosis immunology in children: diagnostic and therapeutic challenges and opportunities. Int J Tuberc Lung Dis 2004;8:658–674.

    PubMed  CAS  Google Scholar 

  67. ATS/CDC Statement Committee on Latent Tuberculosis Infection: Targeted tuberculin testing and treatment of latent tuberculosis infection. MMWR 2000;49:1–54.

    Google Scholar 

  68. Prevention Committee of Japanese Society of Tuberculosis, Japanese College of Rheumatology. Recommendation for more active use of chemoprophylaxis of tuberculosis (in Japanese). Kekkaku 2004;79:747–748.

    Google Scholar 

  69. American Thoracic Society, CDC: The tuberculin skin test. Am Rev Respir Dis 1981;124:356–363.

    Google Scholar 

  70. Ferrara G, Losi M, Meacci M, Meccugni B, Piro R, Roversi P, et al. Routine hospital use of a new commercial whole blood interferon-γ assay for the diagnosis of tuberculosis infection. Am J Respir Crit Care Med 2005;172:631–635.

    Article  PubMed  Google Scholar 

  71. Kobashi Y, Mouri K, Obase Y, Fukuda M, Miyashita N, Oka M. Clinical evaluation of QuantiFERON TB-2G test for immunocompromised patients. Eur Respir J. 2007; doi: 10.1183/09031936.0004.

  72. Mori T, Harada N, Higuchi K, Sekiya Y, Uchimura K, Shimao T. Waning of the specific interferon-gamma response after years of tuberculosis infection. Int J Tuberc Lung Dis 2007;11:1021–1025.

    PubMed  CAS  Google Scholar 

  73. Raby E, Moyo M, Devendra A, Banda J, De Haas P, Ayles H, Godfrey-Faussett P. The effects of HIV on the sensitivity of a whole blood IFN-c release assay in Zambian adults with active tuberculosis. PLoS One 2008;3:e2489.

    Article  PubMed  Google Scholar 

  74. Vincenti D, Cararra S, Butera FO, et al. Response to region of difference 1 (RD1) epitope in human immunodeficiency virus (HIV)-infected individuals enrolled with suspected active tuberculosis: a pilot study. Clin Exp Immunol 2007;150:91–98.

    PubMed  CAS  Google Scholar 

  75. Nagai H, Kawabe Y, Ariga H, Shigeyama F, et al. Usefulness of a whole blood interferon-gamma assay (QuantiFERON-TB 2G) for detecting tuberculosis infection in HIV-infected persons (in Japanese). Kekkaku 2007;82:635–640.

    PubMed  CAS  Google Scholar 

  76. Talati NJ. Comparison of tuberculin skin test and interferon-gamma release assays for diagnosis of latent TB infection among HIV positive persons. In: Rethinking the epidemiology of tuberculosis infection. Vancouver, February 21, 22, 2007.

  77. Luetkemeyer AF, Charlebois ED, Flores LL, Bangsberg DR, Deeks SG, Martin JN, Havlir DV. Comparison of an interferon-{gamma} release assay to tuberculin skin testing in hiv-infected individuals. Am J Respir Crit Care Med 2007;75:737–742.

    Article  Google Scholar 

  78. Jones S, de Gijsel D, Wallach FR, Gurtman AC, Shi Q, Sacks H. Utility of QuantiFERON-TB Gold in-tube testing for latent TB infection in HIV-infected individuals. Int J Tuberc Lung Dis 2007;11:1190–1195.

    PubMed  CAS  Google Scholar 

  79. Brock I, Ruhwald M, Lundgren B, Westh H, Mathiesen LR, Ravn P. Latent tuberculosis in HIV positive, diagnosed by the M. tuberculosis specific interferon-γ test. Respir Res 2006;7:56 doi:10.1186/1465-9921-7-56.

    Article  PubMed  Google Scholar 

  80. Stephan C, Wolf T, Goetsch U, Bellinger O, Nisius G, et al. Comparing QuantiFERON-tuberculosis gold, T-SPOT tuberculosis and tuberculin skin test in HIV-infected individuals from a low prevalence tuberculosis country. AIDS 2008;22:2471–2479.

    Article  PubMed  Google Scholar 

  81. Balcells ME, Perez CM, Chanqueo L, Lasso M, et al. A comparative study of two different methods of the detection of latent tuberculosis in HIV-positive individuals in Chile. Int J Inf Dis 2008; doi: 10.1016/j.ijid.2008.03.005.

  82. Hammond AS, McConkey SJ, Hill P, Crozier S, et al. Mycobacterial T cell responses in HIV-infected patients with advanced immunosuppression. J Infect Dis 2008;197:295–299.

    Article  PubMed  CAS  Google Scholar 

  83. Hans Rider HL, Cauthen GM, Comstock GW, Snider DE. Epidemiology of tuberculosis in the United States. Epidemiol Rev 1989;11:79–98.

    Google Scholar 

  84. Keane J, Gershon S, Wise RP, et al. Tuberculosis associated with infliximab, a tumor necrosis factor-alpha neutralizing agent. N Engl J Med 2001;345:1098–1104.

    Article  PubMed  CAS  Google Scholar 

  85. Matsumoto T, Tanaka T, Kawase I. Infliximab for rheumatoid arthritis in a patient with tuberculosis. New Engl J Med 2006;355:740–741.

    Article  PubMed  CAS  Google Scholar 

  86. Centers for Disease Control and Prevention. Tuberculosis associated with blocking agents against tumor necrosis factor-alpha — California, 2002–2003. MMWR Morb Mortal Wkly Rep 2004;53:683–686.

    Google Scholar 

  87. Matulis G, Jüni P, Villiger PM, Gadola SD. Detection of latent tuberculosis in immunosuppressed patients with autoimmune diseases: performance of a Mycobacterium tuberculosis antigenspecific interferon gamma assay. Ann Rheum Dis 2008;67:84–90. Epub 2007.

    Article  PubMed  CAS  Google Scholar 

  88. Chen DY, Shen GH, Hsieh TY, Hsieh CW, Lan JL. Effectiveness of the Combination of a whole-blood interferon-gamma assay and the tuberculin skin test in detecting latent tuberculosis infection in rheumatoid arthritis patients receiving adalimumab therapy. Arthritis Rheum 2008;59:800–806.

    Article  PubMed  CAS  Google Scholar 

  89. Schoepfer AM, Flogerzi B, Fallegger S, Schaffer T, et al. Comparison of interferon-gamma release assay versus tuberculin skin test for tuberculosis screening in inflammatory bowel disease. Am J Gastroenterol 2008;103:1–8.

    Article  Google Scholar 

  90. Inamoto H. Tuberculosis in dialysis patients. 3. Epidemiology of pulmonary tuberculosis. Kekkaku 1987;57:477–481.

    Google Scholar 

  91. Winthrop KL, Nyendak M, Calvet H, Oh P, et al. Interferon-Release assays for diagnosing Mycobacterium tuberculosis infection in renal dialysis patients. Clin J Am Soc Nephrol 2008;3:1357–1363.

    Article  PubMed  Google Scholar 

  92. Lee SSJ, Chou KJ, Su IJ, Chen YS, et al. High Prevalence of latent tuberculosis infection in patients in end-stage renal disease on hemodialysis: comparison of QuantiFERON-TB GOLD, ELISPOT, and tuberculin skin test. Infection 2008. DOI 10.1007/s15010-008-8082-3.

  93. Ulrichs T, Anding R, Kaufmann SH, Munk ME. Numbers of IFN-gamma-producing cells against ESAT-6 increase in tuberculosis patients during chemotherapy. Int J Tuberc Lung Dis 2000;4:1181–1183.

    PubMed  CAS  Google Scholar 

  94. Lalvani A, Nagvenkar P, Udwadia Z, Pathan AA, Wilkinson KA, Shastri JS, et al. Enumeration of T cells specific for RD1-encoded antigens suggests a high prevalence of latent Mycobacterium tuberculosis infection in healthy urban Indians. J Infect Dis 2001;183:469–477.

    Article  PubMed  CAS  Google Scholar 

  95. Pathan AA, Wilkinson KA, Klenerman P, McShane H, Davidson RN, Pasvol G, et al. Direct ex vivo analysis of antigen-specific IFN gamma-secreting CD4 T cells in Mycobacterium tuberculosis-infected individuals: associations with clinical disease state and effect of treatment. J Immunol 2001;167:5217–5225.

    PubMed  CAS  Google Scholar 

  96. Al-Attiyah R, Mustafa AS, Abal AT, Madi NM, Andersen P. Restoration of mycobacterial antigen-induced proliferation and interferon-gamma responses in peripheral blood mononuclear cells of tuberculosis patients upon effective chemotherapy. FEMS Immunol Med Microbiol 2003;38:249–256.

    Article  PubMed  CAS  Google Scholar 

  97. Carrara S, Vincenti D, Petrosillo N, Amicosante M, Girardi E, Goletti D. Use of a T-cell-based assay for monitoring efficacy of antituberculosis therapy. Clin Infect Dis 2004;38:754–756.

    Article  PubMed  CAS  Google Scholar 

  98. Ferrand RA, Bothamley GH, Whelan A, Dockrell HM. Interferon-gamma responses to ESAT-6 in tuberculosis patients early into and after anti-tuberculosis treatment. Int J Tuberc Lung Dis 2005;9:1034–1039.

    PubMed  CAS  Google Scholar 

  99. Kobashi Y, Obase Y, Fukuda M, Yoshida K, et al. Clinical reevaluation of the QuantiFERON TB-2G test as a diagnostic method for differentiating active tuberculosis from nontuberculous mycobacteriosis. Clin Infect Dis 2006;43:1540–1546.

    Article  PubMed  Google Scholar 

  100. Aiken AM, Hill PC, Fox A, McAdam KP, Jackson-Sillah DJ, Lugos MD, et al. Reversion of the ELISPOT test after treatment in Gambian tuberculosis cases. BMC Infect Dis 2006;6:66.

    Article  PubMed  Google Scholar 

  101. Pai M, Joshi R, Dogra S, Mendiratta DK, Narang P, Dheda K, Kalantri S. Persistently elevated T cell interferon-γ responses after treatment for latent tuberculosis infection among health care workers in India: a preliminary report. J Occup Med Toxicol. 2006;1:7 doi:10.1186/1745-6673-1-7.

    Article  PubMed  Google Scholar 

  102. Wilkinson KA, Kon OM, Newton SM, Meintjes G, Davidson RN, Pasvol G, Wilkinson RJ. Effect of treatment of latent tuberculosis infection on the T cell response to Mycobacterium tuberculosis antigens. J Infect Dis 2006;193:354–359.

    Article  PubMed  CAS  Google Scholar 

  103. Ewer K, Millington KA, Deeks JJ, Alvarez L, et al. Dynamic antigen-specific T-cell responses after point-source exposure to Mycobacterium tuberculosis. Am J Respir Crit Care Med 2006;174:831–839.

    Article  PubMed  CAS  Google Scholar 

  104. Higuchi K, Harada N, Mori T. Interferon-γ responses after isoniazid chemotherapy for latent tuberculosis. Respirology 2008;13:468–472.

    Article  PubMed  Google Scholar 

  105. Franken WPJ, Arend SM, Thijsen SFT, Bouwman JJM, et al. Interferon-gamma release assays during follow-up of tuberculin skin test-positive contacts. Int J Tuberc Lung Dis 2008;12:1286–1294.

    PubMed  CAS  Google Scholar 

  106. Dheda K, Pooran A, Pai M, Miller RF, Lesley K, Booth HL, et al. Interpretation of Mycobacterium tuberculosis antigen-specific IFN-γ release assays (T-SPOT-TB) and factors that may modulate test results. J Infect 2007;55:169–173.

    Article  PubMed  CAS  Google Scholar 

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  1. Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo, 204-8533, Japan

    Toru Mori

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Mori, T. Usefulness of interferon-gamma release assays for diagnosing TB infection and problems with these assays. J Infect Chemother 15, 143–155 (2009). https://doi.org/10.1007/s10156-009-0686-8

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  • DOI: https://doi.org/10.1007/s10156-009-0686-8

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