Summary
Genistein, an inhibitor of protein-tyrosine kinase, inhibited the replication of herpes simplex virus type 1 (HSV-1) at genistein concentrations of more than 25 µM, whereas the related compounds, which do not inhibit protein-tyrosine kinases, did not affect the replication of HSV-1. In the presence of genistein, the phosphorylation of tyrosine residues in specific viral polypeptides was markedly reduced. These results indicate that the phosphorylation of tyrosine residues in viral polypeptides may be essential for the replication of HSV-1.
References
Aaronson SA (1991) Growth factors and cancer. Science 254: 1146–1153
Ackermann M, Braun DK, Pereira L, Roizman B (1984) Characterization of herpes simplex virus 1 α protein 0, 4, and 27 with monoclonal antibodies. J Virol 52: 108–118
Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh N, Shibuya M, Fukami Y (1987) Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem 262: 5592–5595
Baird A, Florkiewicz RZ, Maher PA, Kaner RJ, Hajjar DP (1990) Mediation of virion penetration into vascular cells by association of basic fibroblast growth factor with herpes simplex virus type 1. Science 348: 344–346
Bank LM, Halliburton IW, Purifoy DJM, Killington RA, Powell KL (1985) Studies on the herpes simplex virus alkaline nuclease: detection of type-common and type-specific epitopes on the enzyme. J Gen Virol 66: 1–14
Campbell MEM, Palfreyman JW, Preston CM (1984) Identification of herpes simplex virus DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcription. J Mol Biol 180: 1–19
Cohen DI, Tani Y, Tian H, Boone E, Samelson LE, Lane HC (1992) Participation of tyrosine phosphorylation in the cytopathic effect of human immunodeficiency virus-1. Science 256: 542–545
Cunningham C, Davison AJ, Dolan A, Frame MC, McGeoch DJ, Meredith DM, Moss HWM, Orr AC (1992) The UL 13 virion protein of herpes simplex virus type 1 is phorphorylated by a novel virus-induced protein kinase. J Gen Virol 73: 303–311
Elliott GD, Meredith DM (1992) The herpes simplex virus type 1 tegument protein VP 22 is encoded by gene UL 49. J Gen Virol 73: 723–726
Fenwick ML, Walker MJ (1979) Phosphorylation of a ribosomal protein and of virus-specific proteins in cells infected with herpes simplex virus. J Gen Virol 45: 397–405
Honess RW, Roizman B (1973) Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and non-structural herpes virus polypeptides in the infected cell. J Virol 12: 1347–1365
Hope RG, Marsden HS (1983) Processing of glycoproteins induced by herpes simplex virus type 1: Sulfation and nature of the oligosaccharide linkages. J Gen Virol 64: 1943–1953
Hunter T, Cooper JA (1985) Protein-tyrosine kinases. Annu Rev Biochem 54: 897–930
Kennedy IM, Stevely WS, Leader DP (1981) Phosphorylation of ribosomal proteins in hamster fibroblasts infected with pseudorabies virus or herpes simplex virus. J Virol 39: 359–366
Lemaster S, Roizman B (1980) Herpes simplex virus phosphoproteins. II. Characterization of the virion protein kinase and of the polypeptides phosphorylated in the virion. J Virol 35: 798–811
Linassier C, Pierre M, Le Pecq J-B, Pierre J (1990) Mechanism of action of genistein, an inhibitor of EGF receptor tyrosine kinase activity. Biochem Pharmacol 39: 187–193
Nishiyama Y, Fujioka H, Tsurumi T, Yamamoto N, Maeno K, Yoshida S, Shimokata K (1987) Effects of the epipodophyllotoxin V-16-213 on herpes simplex virus type 2 replication. J Gen Virol 68: 913–918
Okura A, Arakawa H, Oka H, Yoshinari T, Monden Y (1988) Effect of genistein on topoisomerase activity and on the growth of [Val 12]Ha-ras-transformed NIH 3T3 cells. Biochem Biophys Res Commun 157: 183–189
Pereira L, Wolff MH, Fenwick M, Roizman B (1977) Regulation of herpesvirus macromolecular synthesis. V. Properties of α-polypeptides made in HSV-1 and HSV-2. Virology 77: 733–749
Preston VG, Palfreyman JW, Dutia BM (1984) Identification of a herpes simplex virus type 1 polypeptide which is a component of the virus-induced ribonucleotide reductase. J Gen Virol 65: 1457–1466
Purves FC, Spector D, Roizman B (1991) The herpes simplex virus 1 protein kinase encoded by the US 3 gene mediates post-translational modification of the phosphoprotein encoded by the UL 34 gene. J Virol 65: 5757–5764
Spear PG (1985) Glycoproteins specified by herpes simplex viruses. In: Roizman B (ed) The herpesvirus, vol 3. Plenum, New York, pp 315–356
Watanabe T, Kondo K, Oishi M (1991) Induction of in vitro differentiation of mouse erythroleukemia cells by genistein, an inhibitor of tyrosine protein kinases. Cancer Res 51: 764–768
Wilcox KW, Kohn A, Sklyanskaya E, Roizman B (1980) Herpes simplex virus phosphoproteins. I. Phosphate cycles on and off some viral polypeptides and can alter their affinity for DNA. J Virol 33: 167–182
Yamada Y, Yamamoto N, Maeno K, Nishiyama Y (1990) Role of DNA topoisomerase I in the replication of herpes simplex virus type 2. Arch Virol 110: 121–127
Yura Y, Terashima K, Iga H, Yanagawa T, Yoshida H, Hayashi Y, Sato M (1987) Macromolecular synthesis at early stage of herpes simplex virus type 2 (HSV-2) latency in a human neuroblastoma cell line IMR-32: repression of late viral polypeptide synthesis and accumulation of cellular heat-shock proteins. Arch Virol 96: 17–28
Yura Y, Iga H, Kondo Y, Harada K, Tsujimoto H, Yanagawa T, Yoshida H, Sato M (1992) Heparan sulfate as a mediator of herpes simplex virus binding to basement membrane. J Invest Dermatol 98: 494–498
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Yura, Y., Yoshida, H. & Sato, M. Inhibition of herpes simplex virus replication by genistein, an inhibitor of protein-tyrosine kinase. Archives of Virology 132, 451–461 (1993). https://doi.org/10.1007/BF01309554
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DOI: https://doi.org/10.1007/BF01309554