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Degradation pathways of cyclic alkanes in Rhodococcus sp. NDKK48

  • Applied Microbial and Cell Physiology
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Abstract

The degradation pathways for cyclic alkanes (c-alkanes) in Rhodococcus sp. NDKK48 were investigated. Strain NDKK48 used dodecylcyclohexane as a sole carbon and energy source, and five metabolites in the dodecylcyclohexane degradation pathway were detected by gas-chromatography/mass spectra. The metabolites were identified as cyclohexanecarboxylic acid, cyclohexylacetic acid, 1-cyclohexene-1-acetic acid, 4-dodecylcyclohexanol, and 4-dodecylcyclohexanone. The strain degrades dodecylcyclohexane via a ring oxidation pathway and an alkyl side chain oxidation pathway. Cyclohexanecarboxylic acid was further oxidized to muconic acid via 1-cyclohexene-1-carboxylic acid and benzoic acid, and the muconic acid was finally used by strain NDKK48 for growth. Methylcyclohexane and cyclohexane were co-oxidized with hexadecane by strain NDKK48. Methylcyclohexane was degraded via a ring oxidation pathway, and the degradation pathway contained part of the Baeyer-Villiger oxidation for ring cleavage. Cyclohexane was also degraded by the same pathway as methylcyclohexane. Thus, strain NDKK48 has two pathways for the complete degradation of c-alkanes.

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Authors and Affiliations

  1. Department of Bioscience and Technology, Faculty of Science and Engineering, Ritsumeikan University, Nojihigashi 1-1-1, Kusatsu, Shiga, 525-8577, Japan

    D. Koma, Y. Sakashita, K. Kubota, Y. Fujii & M. Kubo

  2. Department of Biochemistry, Numazu College of Technology, Ooka 3600, Numazu, Shizuoka, 410-8501, Japan

    F. Hasumi

  3. Department of Environmental Engineering, College of Engineering, Chonnam National University, Kwangju, 500757, South Korea

    S. Y. Chung

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  1. D. Koma
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  2. Y. Sakashita
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  3. K. Kubota
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  4. Y. Fujii
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  5. F. Hasumi
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  6. S. Y. Chung
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  7. M. Kubo
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Correspondence to M. Kubo.

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Koma, D., Sakashita, Y., Kubota, K. et al. Degradation pathways of cyclic alkanes in Rhodococcus sp. NDKK48. Appl Microbiol Biotechnol 66, 92–99 (2004). https://doi.org/10.1007/s00253-004-1623-5

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  • DOI: https://doi.org/10.1007/s00253-004-1623-5

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