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Qualitative and Quantitative Studies on Impurities in Schizonepetin, a Novel Antiviral Agent, Using HPLC, NMR and MS

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Abstract

Schizonepetin is a new naturally occurring monoterpene with antiviral activity. Three unknown related impurities were observed in analysis of schizonepetin bulk drug. A semi-preparative liquid chromatography was used for isolation of the three impurities. Three impurities were characterized as (−)-mintlactone (Impurity I), (R)-(+)-pulegone (Impurity II) and (+)-menthofuran (Impurity III) by a variety of spectral data (IR, UV, MS, 1H-NMR, 13C-NMR, DEPT and 2D-NMR). The simultaneous quantitative determination of schizonepetin and its impurities (Imp-I, II and III) was performed by reversed-phase HPLC method with UV detection at 220 nm. The method shows good precision, sensitivity, linearity (between 0.025 and 1.0 mg mL−1) and robustness.

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Acknowledgments

This work was financially sponsored by National Nature Science and Technology Major Project of China (Project 2009ZX09103-339). We thank Dr. Yanqin Duan from Department of Medicinal Chemistry, China Pharmaceutical University, for assistance in preparation of the manuscript.

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Authors and Affiliations

  1. Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, 210046, Jiangsu, China

    Dongliang Liu, Mingqiu Shan, Peidong Chen, Li Zhang & Anwei Ding

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  1. Dongliang Liu
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  2. Mingqiu Shan
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  3. Peidong Chen
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  4. Li Zhang
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  5. Anwei Ding
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Correspondence to Anwei Ding.

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Liu, D., Shan, M., Chen, P. et al. Qualitative and Quantitative Studies on Impurities in Schizonepetin, a Novel Antiviral Agent, Using HPLC, NMR and MS. Chromatographia 76, 491–498 (2013). https://doi.org/10.1007/s10337-013-2424-8

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  • DOI: https://doi.org/10.1007/s10337-013-2424-8

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