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Thyroid Hormone-Induced Alterations in Membrane Structure-Function Relationships: Studies on Kinetic Properties of Rat Kidney Microsomal Na+,K+-ATPase and Lipid/Phospholipid Profiles

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Abstract

The effects of thyroidectomy (Tx) and subsequent treatment with 3,5,3′-triiodothyronine (T3) or combined replacement therapy (TR) with T3 and thyroxine (T4) on the substrate and temperature kinetics properties of Na+,K+-ATPase and lipid/phospholipid makeup of rat kidney microsomes were examined. Enzyme activity was somewhat high in the hypothyroid (Tx) animals and increased significantly following T3 treatment, while TR treatment caused a decrease. In the Tx and T3 groups enzyme activity resolved in two kinetic components, while in the TR group the enzyme showed allosteric behavior up to 0.5 mm ATP concentration. The K m and V max values of both the components decreased in Tx animals without affecting the catalytic efficiency. T3 treatment caused a significant increase in the V max of both the components, with a significant increase in the catalytic efficiency, while the K m values were not upregulated. The TR regimen lowered the K m and V max of component II but improved the catalytic efficiency. Thyroid status-dependent changes were also noted in the temperature kinetics of the enzyme. Regression analysis revealed that changes in the substrate and temperature kinetics parameters correlated with specific phospholipid components.

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References

  • Arystarkhova E, Sweadner KJ (1997) Tissue-specific expression of the Na,K-ATPase beta3 subunit. The presence of beta3 in lung and liver addresses the problem of the missing subunit. J Biol Chem 272:22405–22408

    Article  PubMed  CAS  Google Scholar 

  • Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 234:466–468

    PubMed  CAS  Google Scholar 

  • Blanco G, Mercer RW (1998) Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function. Am J Physiol 275:F633–F650

    PubMed  CAS  Google Scholar 

  • Chaudhury S, Ismail-Beigi F, Gick GG, Levenson R, Edelman IS (1987) Effect of thyroid hormone on the abundance of Na,K-adenosine triphosphatase alpha-subunit messenger ribonucleic acid. Mol Endocrinol 1:83–89

    PubMed  CAS  Google Scholar 

  • Dixon M, Webb EC (1979) Enzyme kinetics. In: Dixon M, Webb EC, Thorne C Jr, Tipton KF (eds), Enzymes, 3rd ed. London: Longman, pp 47–206

  • Escobar-Morreale HF, del Rey FE, Obregon MJ, de Escobar GM (1996) Only the combined treatment with thyroxine and triiodothyronine ensures euthyroidism in all tissues of the thyroidectomized rat. Endocrinology 137:2490–2502

    Article  PubMed  CAS  Google Scholar 

  • Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

    PubMed  CAS  Google Scholar 

  • Garg LC, Tisher CC (1985) Effects of thyroid hormone on Na-K-adenosine triphosphatase activity along the rat nephron. J Lab Clin Med 106:568–572

    PubMed  CAS  Google Scholar 

  • Gick GG, Ismail-Beigi F, Edelman IS (1988) Thyroidal regulation of rat renal and hepatic Na,K-ATPase gene expression. J Biol Chem 263:16610–16618

    PubMed  CAS  Google Scholar 

  • Hoch FL (1988) Lipids and thyroid hormones. Prog Lipid Res 27:199–270

    Article  PubMed  CAS  Google Scholar 

  • Holmes EW Jr, Di Scala VA (1970) Studies on the exaggerated natriuretic response to a saline infusion in the hypothyroid rat. J Clin Invest 49:1224–1236

    PubMed  CAS  Google Scholar 

  • Katewa SD, Katyare SS (2003a) Kinetic attributes of rat liver microsomal adenosine 5′ triphosphate phophohydrolase (ATPase). Indian J Biochem Biophys 40:252–259

    CAS  Google Scholar 

  • Katewa SD, Katyare SS (2003b) A simplified method for inorganic phosphate determination and its application for phosphate analysis in enzyme assays. Anal Biochem 323:180–187

    Article  PubMed  CAS  Google Scholar 

  • Katyare SS, Rajan RR (2005) Influence of thyroid hormone treatment on respiratory activity of cerebral mitochondria from hypothyroid rats. A critical re-assessment. Exp Neurol 195:416–422

    Article  PubMed  CAS  Google Scholar 

  • Katyare SS, Joshi MV, Fatterpaker P, Sreenivasan A (1977) Effect of thyroid deficiency on oxidative phosphorylation in rat liver, kidney and brain mitochondria. Arch Biochem Biophys 182:155–163

    Article  PubMed  CAS  Google Scholar 

  • Katz AI, Lindheimer MD (1973) Renal sodium- and potassium-activated adenosine triphosphatase and sodium reabsorption in the hypothyroid rat. J Clin Invest 52:796–804

    Article  PubMed  CAS  Google Scholar 

  • Katz AI, Emmanouel DS, Lindheimer MD (1975) Thyroid hormone and the kidney. Nephron 15:223–249

    Article  PubMed  CAS  Google Scholar 

  • Kaushal R, Dave KR, Katyare SS (1999) Paracetamol hepatotoxicity and microsomal function. Environ Toxicol Pharmacol 1:67–74

    Article  Google Scholar 

  • Kinsella J, Sacktor B (1985) Thyroid hormones increase Na+-H+ exchange activity in renal brush border membranes. Proc Natl Acad Sci USA 82:3606–3610

    Article  PubMed  CAS  Google Scholar 

  • Lo S-C, August TR, Liberman UA, Edelman IS (1976) Dependence of renal (Na+,K+)-adenosine triphosphatase activity on thyroid status. J Biol Chem 251:7826–7833

    PubMed  CAS  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  • McDonough AA, Brown TA, Horowitz B, Chiu R, Schlotterbeck J, Bowen J, Schmitt CA (1988) Thyroid hormone coordinately regulates Na+-K+-ATPase alpha- and beta-subunit mRNA levels in kidney. Am J Physiol 254:C323–C329

    PubMed  CAS  Google Scholar 

  • Michael UF, Barenberg RL, Chavez R, Vaamonde CA, Papper S (1972) Renal handling of sodium and water in the hypothyroid rat. Clearance and micropuncture studies. J Clin Invest 51:1405–1412

    PubMed  CAS  Google Scholar 

  • Modi HR, Patel SP, Katyare SS, Patel MA (2007) Thyroid hormone treatments differentially affect kinetic properties of FoF1 ATPase and succinate oxidase and lipid/phospholipid composition of rat kidney mitochondria. A correlative study. J Membr Biol (in press)

  • Pandya JD, Dave KR, Katyare SS (2004) Effect of long-term aluminum feeding on lipid/phospholipid profiles of rat brain myelin. Lipids Health Dis 3:13–18

    Article  PubMed  CAS  Google Scholar 

  • Patel HG, Aras RV, Dave KR, Katyare SS (2000) Kinetic attributes of Na+,K+-ATPase and lipid/phospholipid profiles of rat and human erythrocyte membrane. Z Naturforsch [C] 55:770–777

    CAS  Google Scholar 

  • Patel SP, Katyare SS (2006a) Insulin-status-dependant alterations in lipid/phospholipid composition of rat kidney microsomes and mitochondria. Lipids 41:819–825

    Article  PubMed  CAS  Google Scholar 

  • Patel SP, Katyare SS (2006b) Insulin-status-dependant modulation of FoF1 ATPase activity in rat kidney mitochondria. Arch Physiol Biochem 112:150–157

    Article  PubMed  CAS  Google Scholar 

  • Robinson JD, Flashner MS (1979) The (Na+,K+)-activated ATPase. Enzymatic and transport properties. Biochim Biophys Acta 549:145–176

    PubMed  CAS  Google Scholar 

  • Satav JG, Katyare SS (1991) Effect of thyroidectomy and subsequent treatment with triiodothyronine on kidney mitochondrial oxidative phosphorylation in the rat. J Biosci 16:81–89

    Article  CAS  Google Scholar 

  • Skipski VP, Peterson RF, Barclay M (1964) Lipid composition of human serum lipoprotein. Biochem J 90:374–378

    PubMed  CAS  Google Scholar 

  • Tata JR (1964) Biological action of thyroid hormones at the cellular and molecular levels. In: Litwack G, Kritchevsky D (eds), Action of Hormones on Molecular Processes. New York: Wiley, pp 58–131

    Google Scholar 

  • Wetzel RK, Arystarkhova E, Sweadner KJ (1999) Cellular and subcellular specification of Na,K-ATPase alpha and beta isoforms in the postnatal development of mouse retina. J Neurosci 19:9878–9889

    PubMed  CAS  Google Scholar 

  • Zlatkis A, Zak B, Boyle AJ (1953) A new method for the direct determination of serum cholesterol. J Lab Clin Med 41:486–492

    PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390 002, India

    Surendra S. Katyare, Hiren R. Modi, Samir P. Patel & Minal A. Patel

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  1. Surendra S. Katyare
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  2. Hiren R. Modi
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  3. Samir P. Patel
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  4. Minal A. Patel
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Correspondence to Hiren R. Modi.

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Katyare, S.S., Modi, H.R., Patel, S.P. et al. Thyroid Hormone-Induced Alterations in Membrane Structure-Function Relationships: Studies on Kinetic Properties of Rat Kidney Microsomal Na+,K+-ATPase and Lipid/Phospholipid Profiles. J Membrane Biol 219, 71–81 (2007). https://doi.org/10.1007/s00232-007-9063-7

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  • DOI: https://doi.org/10.1007/s00232-007-9063-7

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