Skip to main content

Advertisement

SpringerLink
Log in

Resveratrol prevents hepatic steatosis induced by hepatitis C virus core protein

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Hepatitis C virus (HCV) core protein plays an important role in the development of hepatic steatosis in patients with chronic HCV infection. Treatment of C57BL/6 mice infected with HCV core recombinant adenoviruses with resveratrol significantly decreased hepatic triacylglycerols (TAG) while the serum TAG level was unaffected. RT-PCR and Western blotting showed that HCV core protein attenuated the expression of Sirt1 and PPAR-α, which would be reversed by resveratrol. This was also confirmed in primary mouse hepatic cells infected with HCV core protein expressing adenovirus. Thus, resveratrol may prevent against hepatic steatosis by blocking the inhibited expression of Sirt1 and PPAR-α induced by HCV core protein.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Barba G, Harper F, Harada T et al (1997) Hepatitis C virus core protein shows a cytoplasmic localization and associates to cellular lipid storage droplets. Proc Natl Acad Sci USA 94:1200–1205

    Article  PubMed  CAS  Google Scholar 

  • Baur JA, Pearson KJ, Price NL et al (2006) Resveratrol improves health and survival of mice on a high-calorie diet. Nature 444:337–342

    Article  PubMed  CAS  Google Scholar 

  • Bordone L, Guarente L (2005) Calorie restriction, SIRT1 and metabolism: understanding longevity. Nat Rev Mol Cell Biol 6:298–305

    Article  PubMed  CAS  Google Scholar 

  • Borra MT, Smith BC, Denu JM (2005) Mechanism of human SIRT1 activation by resveratrol. J Biol Chem 280:17187–17195

    Article  PubMed  CAS  Google Scholar 

  • Bujanda L, Hijona E, Larzabal M et al (2008) Resveratrol inhibits nonalcoholic fatty liver disease in rats. BMC Gastroenterol 8:40

    Article  PubMed  Google Scholar 

  • Cohen HY, Miller C, Bitterman KJ et al (2004) Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Science 305:390–392

    Article  PubMed  CAS  Google Scholar 

  • Delladetsima JK, Rassidakis G, Tassopoulos NC, Papatheodoridis GV, Smyrnoff T, Vafiadis I (1996) Histopathology of chronic hepatitis C in relation to epidemiological factors. J Hepatol 24:27–32

    Article  PubMed  CAS  Google Scholar 

  • Kasdallah-Grissa A, Mornagui B, Aouani E et al (2006) Protective effect of resveratrol on ethanol-induced lipid peroxidation in rats. Alcohol Alcohol 41:236–239

    PubMed  CAS  Google Scholar 

  • Lefkowitch JH, Schiff ER, Davis GL et al (1993) Pathological diagnosis of chronic hepatitis C: a multicenter comparative study with chronic hepatitis B. The Hepatitis Interventional Therapy Group. Gastroenterology 104:595–603

    PubMed  CAS  Google Scholar 

  • Lonardo A, Adinolfi LE, Loria P, Carulli N, Ruggiero G, Day CP (2004) Steatosis and hepatitis C virus: mechanisms and significance for hepatic and extrahepatic disease. Gastroenterology 126:586–597

    Article  PubMed  CAS  Google Scholar 

  • Moriya K, Yotsuyanagi H, Shintani Y et al (1997) Hepatitis C virus core protein induces hepatic steatosis in transgenic mice. J Gen Virol 78(Pt 7):1527–1531

    PubMed  CAS  Google Scholar 

  • Nakamura M, Saito H, Ikeda M et al (2010) An antioxidant resveratrol significantly enhanced replication of hepatitis C virus. World J Gastroenterol 16:184–192

    Article  PubMed  CAS  Google Scholar 

  • Tanaka N, Moriya K, Kiyosawa K, Koike K, Gonzalez FJ, Aoyama T (2008) PPARα activation is essential for HCV core protein–induced hepatic steatosis and hepatocellular carcinoma in mice. J Clin Invest 118:683–694

    PubMed  Google Scholar 

  • Ungvari Z, Orosz Z, Rivera A et al (2007) Resveratrol increases vascular oxidative stress resistance. Am J Physiol Heart Circ Physiol 292:H2417–H2424

    Article  PubMed  CAS  Google Scholar 

  • Yamaguchi A, Tazuma S, Nishioka T et al (2005) Hepatitis C virus core protein modulates fatty acid metabolism and thereby causes lipid accumulation in the liver. Dig Dis Sci 50:1361–1371

    Article  PubMed  CAS  Google Scholar 

  • Yilmaz O, Keser S, Tuzcu M, Cetintas B (2007) Resveratrol (trans-3,4′,5-trihydroxystilbene) decreases lipid peroxidation level and protects antioxidant capacity in sera and erythrocytes of old female Wistar rats induced by the kidney carcinogen potassium bromate. Environ Toxicol Pharmacol 24:79–85

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by Grants from National Natural Science Foundation of China (81170798 and 81000171).

Conflicts of interests

No conflicts of interest are involved in this manuscript

Author information

Authors and Affiliations

  1. State Key Laboratory of Cancer Biology and Department of Pathology, Xijing Hospital, Fourth Military Medical University, No.17, Changle West Road, Xi’an, 710032, Shaanxi, China

    Lina Jiang, Yu Gu, Jing Ye, Fang Liu, Chao Wang, Yuqiao Xu, Xiangmei Cao, Liying Zhang, Jing Wang, Hui Wang & Qing Li

  2. Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi’an, 710038, China

    Yilin Zhao

  3. Department of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, China

    Wenpeng Dong

  4. Department of Oncology, Tangdu Hospital, Fourth Military Medical University, Xi’an, 710038, China

    Fanfan Li

Authors
  1. Lina Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jing Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yilin Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yuqiao Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xiangmei Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Liying Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Wenpeng Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Fanfan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Jing Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Hui Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Qing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qing Li.

Additional information

Lina Jiang and Yu Gu contributed equally to this study.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jiang, L., Gu, Y., Ye, J. et al. Resveratrol prevents hepatic steatosis induced by hepatitis C virus core protein. Biotechnol Lett 34, 2205–2212 (2012). https://doi.org/10.1007/s10529-012-1034-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-012-1034-0

Keywords

Search

Navigation