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Immunomodulatory Effects of Granulocyte and Monocyte Adsorption Apheresis as a Treatment for Patients with Ulcerative Colitis

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Abstract

Our aim was to understand the mechanism of immunological changes associated with the use of an adsorptive-type extracorporeal device (Adacolumn) that has been developed for selective adsorption of granulocytes and monocytes/macrophages from peripheral blood of patients with active ulcerative colitis. The column is filled with carriers (G-1 beads) that have a diameter of 2 mm and are made of cellulose diacetate. In peripheral blood treated with the G-1 beads or peripheral blood from patients with active ulcerative colitis following granulocyte and monocyte adsorption apheresis, a significant suppression of proinflammatory cytokines (tissue necrosis factor-α, interleukin-1β, interleukin-6, and interleukin-8) production by leukocytes, neutrophil chemotaxis, down-regulation of leukocyte adhesion molecule (L-selectin) and neutrophil adhesion to interleukin-1β-activated endothelial cells were observed. Furthermore, after granulocyte adsorption therapy, the number of CD10-negative premature granulocytes increased, indicating increased turnover of these cells in the circulation. Our observations suggest that selective granulocyte and monocyte adsorption is associated with modified peripheral blood leukocyte function favorable to patients with ulcerative colitis and possibly other autoimmune disorders which reflect leukocyte hyperactivity.

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REFERENCES

  1. Shimoyama T, Sawada K, Hiwatashi N, Sawada T, Matsueda K, Munakata A, Asakura H, Tanaka T, Kasukawa R, Kimura K, Suzuki Y, Nagamachi Y, Muto T, Nagawa H, Iizuka B, Baba S, Nasu M, Kataoka T, Kashiwagi N, Saniabadi AR: Safety and efficacy of granulocyte and monocyte apheresis in patients with active ulcerative colitis: An open-label pilot multicenter study. J Clin Apher 16:1–9, 2001

    Google Scholar 

  2. D'Arrigo C, Candal-Couto JJ, Greer M, Veale DJ, Woof JM: Human neutrophil Fc receptor mediated adhesion under flow: A hallow fiber model of intravascular arrest. Clin Exp Immunol 100:173–179, 1994

    Google Scholar 

  3. van Dullemen HM, van Deventer SJ, Hommes DW, Bijil HA, Woody J: Treatment of Crohn's disease with anti-tumour necrosis factor chimeric monoclonal antibody (cA2). Gastroenterology 109:129–135, 1995

    PubMed  Google Scholar 

  4. Weissmann G: Lysosomal mechanism of tissue injury in arthritis. N Engl J Med 286:141–147, 1972

    PubMed  Google Scholar 

  5. Grisham MB, Granger N: mechanisms of neutrophil-mediated tissue injury. In Inflammatory Bowel Disease. RP MacDermott, WF Stenson (eds). New York, Elsevier, 1992, pp 225–239

    Google Scholar 

  6. Yamada T, Volkmer C, Grisham MB: Antioxidant properties of 5-aminosalicylic acid: Potential mechanism for its antiinflammatory activity. In Trends in Inflammatory Bowel Disease Therapy. CN Williams (ed). Boston, Kluwer Academic Publishers, 1990, pp 73–84

    Google Scholar 

  7. Yamada T, Grisham MB: Role of neutrophil-derived oxidants in the pathogenesis of intestinal inflammation. Klin Wochenschr 69:988–994, 1991

    PubMed  Google Scholar 

  8. Grisham MB, Yamada T: Neutrophils, nitrogen oxides, and inflammatory bowel disease. Neuro-immuno-physiology of the gastrointestinal mucosa. Ann NY Acad Sci 664:103–105, 1992

    PubMed  Google Scholar 

  9. Kurtel H, Granger DN, Tso P, Grisham MB: Vulnerability of intestinal interstitial fluid to oxidant stress. Am J Physiol (Gastrointest Liver Physiol 26) 263:G573–G578, 1992

    PubMed  Google Scholar 

  10. Lloyd LR, Oppenheim JJ: Poly's lament: The neglected role of the polymorphonuclear neutrophil in the afferent limb of the immune response. Immunol Today 13:169–172, 1992

    PubMed  Google Scholar 

  11. DeForge L, Kenney JS, Jones ML, Warren JS, Remick DG: Biphasic production of IL-8 in lipopolysaccharide (LPS)-stimulated human whole blood. J Immunol 148:2133–2141, 1992

    PubMed  Google Scholar 

  12. Sharon P, Stenson WF: enhanced synthesis of leukotriene B4 by colonic mucosa in inflammatory bowel disease. Gastroenterology 86:453–460, 1984

    PubMed  Google Scholar 

  13. Wengrower D, Liakim R, Karmeli F, Razin E, Rachmilewitz D: Pathogenesis of ulcerative colitis (UC): Enhanced formation of inositol phosphate (IP) and platelet activating factor (PAF). Gastroenterology 92:1691–1695, 1987

    Google Scholar 

  14. Paleolog EM, Hunt M, Elliott MJ, Feldmann M, Maini RN, and Woody JN: Deactivation of vascular endothelium by monoclonal anti-tumor necrosis factor ? antibody in rheumatoid arthritis. Arthritis Rheum 39:1082–1091, 1996

    PubMed  Google Scholar 

  15. Featherstone C: Anti-integrin drugs developed to treat inflammation. The Lancet 347:1106, 1996

    Google Scholar 

  16. Eigler A, Sinha B, Hartmann G, Endres S: Taming TNF: strategies to restrain this proinflammatory cytokine. Immunol Today 18:487–491, 1997

    PubMed  Google Scholar 

  17. Fellermann K, Ludwig D, Stahl M, David-Walek T, Stange EF: Steroid unresponsive attacks of inflammatory bowel disease: Immunomodullation by tacrolimus (FK 506). Am J Gastroenterol 93:1860–1866, 1998

    PubMed  Google Scholar 

  18. Diaz-Gonzalez F, Sanchez-Madrid F: Inhibition of leukocyte adhesion: An alternative mechanism of action for antiinflammatory drugs. Immunology today. 19:169–172, 1998

    PubMed  Google Scholar 

  19. Ohara M, Saniabadi AR, Kokuma S, Hirata I, Adachi M, Agishi T, Kasukawa R: Granulocytapheresis in the treatment of patients with rheumatoid arthritis. Artif Organs 21:989–994, 1997

    PubMed  Google Scholar 

  20. Kashiwagi N, Hirata I, Kasukawa R: A role for granulocyte and monocyte apheresis in the treatment of rheumatoid arthritis. Ther Apher 2:134–141, 1998

    PubMed  Google Scholar 

  21. Boyum A: Isolation of leucocytes from human blood. A two phase system for removal of red cells with methylcellulose as erythrocyte-aggregating agents. Scand J Clin Invest 21(suppl 97):9–29, 1968

    Google Scholar 

  22. Lawrence MB, Mclntire LV, Eskin SG: Effect of flow on polymorphonuclear leukocyte/endothelial cell adhesion. Blood 70:1284–1290, 1987

    PubMed  Google Scholar 

  23. Zigmond SH, Hirsch JG: Leukocyte locomotion and chemotaxis: New methods for evaluation and demonstration of a cell-derived chemotactic factor. J Exp Med 137:387–410, 1973

    PubMed  Google Scholar 

  24. Nicoletti I, Migliorati G, Pagliacci MC: A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Method 139:271–279, 1991

    Google Scholar 

  25. Chofflon M, Juillard C, Juillard P, Gauthier G, Grau GE: Tumor necrosis factor ? production as a possible predictor of relapse in patients with multiple sclerosis. Eur Cytokine Netw 3:523–531, 1992

    PubMed  Google Scholar 

  26. Colotta F, Re F, Polentarutti N, Sozzani S, Mantovani A: Modulation of granulocyte survival and programmed cell death by cytokines and bacterial products. Blood 80:2012–2020, 1992

    PubMed  Google Scholar 

  27. Dransfield I, Stocks SG, Haslett C: Regulation of cell adhesion molecule expression and function associated with neutrophil apoptosis. Blood 85:3246–3253, 1995

    Google Scholar 

  28. Ginis I, Faller DV: Protection from apoptosis in human neutrophils is determined by the surface of adhesion. Am J Physiol 272(Cell Physiol 41):C295–C309, 1997

    PubMed  Google Scholar 

  29. McCormack RT, Nelson RD, Solem LD, LeBien TW: Decreased expression of the common acute lymphoblastic leukaemia antigen (CALLA/CD10) on neutrophils from patients with thermal injury. Br J Haematol 69:189–195, 1988

    PubMed  Google Scholar 

  30. Edwards SW, Hallett MB: Seeing the wood for the tree: the forgotten role of neutrophils in rheumatoid arthritis. Immunol Today 18:320–324, 1997

    PubMed  Google Scholar 

  31. Allison MC, Dhillon AP, Lewis WG, Pounder RE: Pathogenesis of inflammatory bowel disease. In Inflammatory Bowel Disease. MC Allison, AP Dhillon, WG Lewis, RE Pounder (eds). London, Mosby, 1998, pp. 15–22.

    Google Scholar 

  32. Wright V, Amos R: Do drugs change the course of rheumatoid arthritis?. Br Med L 28:964–966, 1980

    Google Scholar 

  33. Springer TA: Traffic signals on endothelium for lymphocyte recirculation and leucocyte emigration. Annu Rev Physiol 57:827–832, 1995

    Article  PubMed  Google Scholar 

  34. Hogg N, Berlin C: Structure and function of adhesion receptors in leucocyte trafficking. Immunol Today 16:327–330, 1995

    PubMed  Google Scholar 

  35. Kato K, Fridman WH, Arata Y, Fridman CS: Conformational changes in the Fc precludes the binding of two Fc?receptors to one IgG. Immunol Today 21:310–312, 2000.

    PubMed  Google Scholar 

  36. Tibble JA, Sigthorsson G, Bridger S, Fagerhol MK, Bjarnason I: Surrogate markers of intestinal inflammation are predictive of relapse in patients with inflammatory bowel disease. Gastroenterology 119:15–22, 2000

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Japan Immunoresearch Laboratories, 351-1 Nishiyokote, Takasaki, Gunma, 370-0021, Japan

    Nobuhito Kashiwagi

  2. First Division of Internal Medicine, Kyoto Prefectural University of Medicine, Japan

    Nobuhito Kashiwagi & Kazuhito Sugimura

  3. Department of Gastroenterology, Jichi Medical School, Japan

    Hirobumi Koiwai & Hironori Yamamoto

  4. First Division of Internal Medicine, Kyoto Prefectural University of Medicine, Japan

    Toshikazu Yoshikawa

  5. Japan Immunoresearch Laboratories, 351-1 Nishiyokote, Takasaki, Gunma, 370-0021, Japan

    Abby R. Saniabadi & Masakazu Adachi

  6. Department of Internal Medicine IV, Hyogo College of Medicine, Japan

    Takashi Shimoyama

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  1. Nobuhito Kashiwagi
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  2. Kazuhito Sugimura
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  3. Hirobumi Koiwai
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  4. Hironori Yamamoto
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  6. Abby R. Saniabadi
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  7. Masakazu Adachi
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  8. Takashi Shimoyama
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Kashiwagi, N., Sugimura, K., Koiwai, H. et al. Immunomodulatory Effects of Granulocyte and Monocyte Adsorption Apheresis as a Treatment for Patients with Ulcerative Colitis. Dig Dis Sci 47, 1334–1341 (2002). https://doi.org/10.1023/A:1015330816364

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