Abstract
Coleus forskohlii hairy root cultures were found to produce forskolin and rosmarinic acid (RA) as the main metabolites. The growth and RA production by C. forskohlii hairy root cultures in various liquid media were examined. The hairy root cultures showed good growth in hormone-free Murashige and Skoog medium containing 3% (w/v) sucrose (MS medium), and Gamborg B5 medium containing 2% (w/v) sucrose (B5 medium). RA yield reached 4.0 mg (MS medium) and 4.4 mg (B5 medium) after 5 weeks of culture in a 100 ml flask containing 20 ml of each medium. Hairy root growth and RA were also investigated after treatment with various concentrations of yeast extract (YE), salicylic acid (SA) and methyl jasmonic acid (MJA). RA production in a 100 ml flask containing 20 ml B5 medium reached 5.4 mg (1.9 times more than control) with treatment of 0.01 or 1% (w/v) YE, 5.5 mg (2.0 times more than control) with treatment of 0.1 mM SA, and the maximum RA content with 9.5 mg per flask (3.4 times more than control) was obtained in the hairy roots treated with 0.1 mM MJA. These results suggest that MJA is an effective elicitor for production of RA in C. forskohlii hairy root cultures.
Similar content being viewed by others
Abbreviations
- B5 medium:
-
Gamborg B5 medium containing 2% (w/v) sucrose
- MJA:
-
methyl jasmonate
- MS medium:
-
Murashige and Skoog medium containing 3% (w/v) sucrose
- NN medium:
-
Nitsch and Nitsch’s medium containing 2% (w/v) sucrose
- RA:
-
rosmarinic acid
- SA:
-
salicylic acid
- WP medium:
-
Woody plant medium containing 2% (w/v) sucrose
- YE:
-
yeast extract
References
N Andarwulan K Shetty (1999) ArticleTitleInfluence of acetyl salicylicacid in combination with fish protein hydrolyzates on hyperhydricity reduction and phenolic synthesis in Origanum vulgare tissue cultures J. Food Biochem. 23 619–635
H Chen F Chen (2000a) ArticleTitleEffect of yeast elicitor on the secondary metabolism of Ti-transformed Salvia miltiorrhiza cell suspension cultures Plant Cell Rep. 19 710–717
H Chen F Chen (2000b) ArticleTitleEffect of yeast elicitor on the growth and secondary metabolism of a high-tanshinone producing line of the Ti-transformed Salvia miltiorrhiza cells in suspension cultures Process Biochem. 35 837–840
H Chen F Chena FCK Chiu CMY Lo (2001) ArticleTitleThe effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza Enzyme Microbial Technol. 28 100–105
OL Gamborg RA Miller K Ojima (1968) ArticleTitleNutrient requirement of suspension cultures of soybean root cultures Exp. Cell Res. 50 151–158
I Hippolyte B Marin JC Baccou R Jonard (1992) ArticleTitleGrowth and rosmarinic acid production in cell suspension cultures of Salvia officinalis L. Plant Cell Rep. 11 109–112
HK Kim SR Oh HK Lee H Huh (2001) ArticleTitleBenzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O Kuntze. Biotechnol. Lett. 23 55–60
G Lloyd B McCown (1980) ArticleTitleCommercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture Int. Plant Prop. Soc. 30 421–427
H Mizukami T Ogawa H Ohashi BE Ellis (1992) ArticleTitleInduction of rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures by yeast extract Plant Cell Rep. 11 480–483
H Mizukami Y Tabira BE Ellis (1993) ArticleTitleMethyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures Plant Cell Rep. 12 706–709
E Motoyama H Tada K Shimomura K Yoshihira K Ishi-maru (1996) ArticleTitleCaffeic acid esters in tissue cultures of Heliotropium pervianum Plant Tiss. Cult. Lett. 13 73–74
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassay with tobacco tissue cultures Physiol. Plant 15 473–497
JP Nitsch C Nitsch (1969) ArticleTitleHaploid plants from pollen grains Science 163 85–87
A Razzaque BE Ellis (1977) ArticleTitleRosmarinic acid production in Coleus cell cultures Planta 137 287–291
K Sasaki A Udagawa H Ishimaru T Hayashi AW Alfermann F Nakanishi K Shimomura (1998) ArticleTitleHigh forskolin production in hairy roots of Coleus forskohlii Plant Cell Rep. 17 457–459
W Sumaryono P Proksch T Hartmann M Nimtz V Wray (1991) ArticleTitleInduction of rosmarinic acid accumulation in cell suspension cultures of Orthosiphon aristatus after treatment with yeast extract Phytochemistry 30 3267–3271
E Szabo A Thelen M Petersen (1999) ArticleTitleFungal elicitor preparations and methyljasmonate enhance rosmarinic acid accumulation in suspension cultures of Coleus blumei Plant Cell Rep. 18 485–489
H Tada Y Murakami T Omoto K Shimomura K Ishimaru (1996) ArticleTitleRosmarinic acid and related phenolics in transformed root cultures of Ocimum basilicum Phytochemistry 42 431–434
MH Zenk H El-Shagi B Ulbrich (1977) ArticleTitleProduction of rosmarinic acid by cell-suspension cultures of Coleus blumei Naturwissen-schaften 64 585–586
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, W., Koike, K., Asada, Y. et al. Rosmarinic acid production by Coleus forskohliihairy root cultures. Plant Cell Tiss Organ Cult 80, 151–155 (2005). https://doi.org/10.1007/s11240-004-9541-x
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11240-004-9541-x