Summary
In the quantitative analysis of cystine and cysteine, before hydrolysis the compounds are often oxidized to cysteic acid with performic acid. The applicability of this process to the quantification of the enantiomers of cystine and cysteine has been examined. An RPHPLC analytical method was developed for determination of the amount of cysteic acid enantiomers and the rate of conversion during oxidation from cystine and cysteine into cysteic acid was determined. Racemization of L-cysteine was not significant during oxidation with performic acid and the process can, therefore, be applied before hydrolysis during quantification of enantiomers of these compounds.
This is a preview of subscription content, log in via an institution to check access.
References
A. J. P. Martin, R. M. L. Synge, Adv. Protein Chem.2, 1 (1945).
T. Yoritaka, T. Ono, Nagasaki Iggakai Zassi29, 400 (1954).
E. Schram, S. Moore, E. I. Bigwood, J. Biol. Chem.57, 33 (1954).
J. L. MacDonald, M. W. Krueger, J. H. Keller, J. Assoc. Off. Anal Chem.68, 826 (1985).
W. H. Hendriks, M. F. Tarttelin, P. J. Moughan, Anim. Sci.67, 165 (1998).
J. Csapó, Elelmiszervizsgálati Közlemények, (Food Investigations)28, 163 (1983).
C. H. W. Hirs, J. Biol. Chem.219, 611 (1956).
S. Einarsson, S. Folestad, B. Josefsson, J. Liquid Chromatogr.10, 1589 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Varga-Visi, E., Terlaky-Balla, E., Pohn, G. et al. RPHPLC determination of L- and D-cystine and cysteine as cysteic acid. Chromatographia 51, S325–S327 (2000). https://doi.org/10.1007/BF02492828
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02492828