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Antiviral activity of green tea catechins against feline calicivirus as a surrogate for norovirus

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Abstract

In this study, several medicinal plant extracts including spices, herb teas, and medical herbs were screened for antiviral activity against feline calicivirus (FCV), a surrogate of norovirus. Among them, a methanolic extract of green tea, Camellia sinensis, only exhibited a significant antiviral activity against FCV. The methanolic extract was further fractionated with several solvents such as methanol (MeOH), n-hexane, chloroform (CHCl3), ethyl acetate (EtOAc), n-butanol (n-BuOH), and water. EtOAc-soluble fraction exhibited the highest antiviral activity against FCV. Moreover, the analysis of the most active fraction using a HPLC led to the identification of 4 known catechins: (-)-epigallocatechin (EGC), (-)-epicatechin (EC), (-)-epigallocatechin gallate (EGCG), and (-)-epicatechin gallate (ECG). Among the catechins tested in this study, EGCG exhibited the most effective antiviral activity (EC50, 12 mg/mL) with relatively low cytotoxicity (CC50, 320 mg/ mL), resulting relatively high selectivity index value 26.67. To our knowledge, this is the first experimental verification showing antiviral activity of catechins from green tea against FCV.

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Authors and Affiliations

  1. Food Safety Research Division, National Fisheries Research and Development Institute, Busan, 619-705, Korea

    Eun-Gyoung Oh, Soon Bum Shin, Kwang-Tae Son, Hee-Jung Lee & Ji Hoe Kim

  2. Department of Microbiology, Pukyong National University, Busan, 608-737, Korea

    Kyoung-Lan Kim & Myung-Suk Lee

  3. Department of Herbal Medicinal Pharmacology, Daegu Haany University, Gyeongsan, Gyeongbuk, 712-715, Korea

    Tae Hoon Kim

  4. Department of Food Science and Technology, Pukyong National University, Busan, 608-737, Korea

    Young-Mog Kim

  5. Department of Life Science and Biotechnology, Dongeui University, Busan, 614-714, Korea

    Eun-Ji Cho, Do-Kyun Kim & Eun-Woo Lee

  6. Blue-Bio Industry RIC, Dongeui University, Busan, 614-714, Korea

    Do-Kyun Kim & Eun-Woo Lee

  7. Faculty of Marine Bioscience & Technology, Gangneung-Wonju National University, Gangwon, 210-702, Korea

    Il-Shik Shin

Authors
  1. Eun-Gyoung Oh
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  2. Kyoung-Lan Kim
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  3. Soon Bum Shin
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  9. Do-Kyun Kim
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  10. Eun-Woo Lee
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  11. Myung-Suk Lee
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  12. Il-Shik Shin
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  13. Ji Hoe Kim
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Correspondence to Ji Hoe Kim.

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Oh, EG., Kim, KL., Shin, S.B. et al. Antiviral activity of green tea catechins against feline calicivirus as a surrogate for norovirus. Food Sci Biotechnol 22, 593–598 (2013). https://doi.org/10.1007/s10068-013-0119-4

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  • DOI: https://doi.org/10.1007/s10068-013-0119-4

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