Abstract
Buphenine (nylidrin), a β-adrenergic agonist, is used therapeutically for its vasodilating effect on the peripheral circulation and possibly on the cerebral circulation as well. In spite of its ophthalmic usage in degenerative retinal disease and glaucoma, buphenine's ocular effects and their mechanisms are not sufficiently established. Consquently, we studied the action of 4.5–120 μM buphenine in isolated, arterially perfused cat eyes, and then used light-evoked electrical signals, perfusion flow rates and the diameters of the retinal vessels as parameters for the drug's effect. Our findings showed that buphenine induced a marked, dose-related, reversible increase in the amplitude of the electroretinogram (ERG) b-wave, a decrease in the c-wave, but no significant changes in the standing potential and light peak of the DC-ERG. The compound action potential of the optic nerve revealed dose-dependent and reversible changes in configuration. However, the flow of perfusate was not affected by the drug, and the diameter of retinal vessels did not change significantly. Our studies suggest that the interaction of buphenine with retinal adrenergic receptors is not related to the vasculature present but to elements involved in information processing. It is likely that these receptors are linked to neurons, since the β-agonist affected the ERG b-waves as well as the compound action potential of the optic nerve.
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References
Bruinink A, Dawis S, Niemeyer G, Lichtensteiger W (1986) Catecholaminergic binding sites in cat retina, pigment epithelium, and choroid. Exp Eye Res 43: 147–151
Chader GJ (1984) Cyclic nucleotides of the retina and pigment epithelium. In: Morgan WW (ed) Retinal transmitters and modulators: models for the brain, vol 1. CRC Press, Boca Raton, Florida, pp 1–62
Cottier D, Niemeyer G (1985) Buphenine changes the electroretinogram but not the flow in perfused cat eyes. Experientia 41: 828
Cottier D, Niemeyer G, Resch H (1986) Einfluss von Buphenin auf die Retina des isolierten Säugetierauges. Klin Monatsbl Augenheilkd 188: 532–536
Dawis S, Hofmann H, Niemeyer G (1985) The electroretinogram, standing potential, and light peak of the perfused cat eye during acid-base changes. Vision Res 25: 1163–1177
Eisenberg S, Camp MF, Horn MR (1960) The effect of nylidrin hydrochloride (Arlidin) on the cerebral circulation. Am J Med Sci 240: 85–92
Freedman L (1955) Arlidin — a new vasodilative sympathomimetic drug. Angiology 6: 52–58
Gouras P, Hoff M (1970) Retinal function in an isolated, perfused mammalian eye. Invest Ophthalmol 9: 388–399
Hadjiconstantinou M, Cohen J, Neff NH (1983) Epinephrine: a potential neurotransmitter in retina. J Neurochem 41: 1440–1444
Jacobson JH, Basar D (1956) The effect of a new drug, Nylidrin, upon the electroretinogram. Arch Ophthalmol 56: 865–868
Laties A (1967) Central retinal artery innervation. Absence of adrenergic innervation to the intraocular branches. Arch Ophthalmol 77: 405–409
Laties AM, Jakobowitz D (1966) A comparative study of the autonomic innervation of the eye in monkey, cat and rabbit. Anat Res 156: 383–395
Nielsen KC, Owman C (1967) Adrenergic innervation of pial arteries related to the circle of Willis in the cat. Brain Res 6: 773–776
Niemeyer G (1973) ERG dependence of flow rate in the isolated and perfused mammalian eye. Brain Res 57: 203–207
Niemeyer G (1975) The function of the retina in the perfused eye. Doc Ophthalmol 39: 53–116
Niemeyer G (1981) Neurobiology of perfused mammalian eyes. J Neurosci Methods 3: 317–337
Niemeyer G (1983) Light modulation of the standing potential in the perfused mammalian eye: characteristics and responses to acidosis. Doc Ophthalmol Proc Ser 37: 41–49
Niemeyer G, Albani C, Schuurmans R (1981) Transmitter-related studies in the isolated, perfused eye of the cat. Vision Res 21: 1661–1663
Niemeyer G, Nagahara K, Demant E (1982) Effects of changes in arterial PO2 and PCO2 on the electroretinogram in the cat. Invest Ophthalmol Vis Sci 23: 678–683
Niemeyer G, Cottier D, Gerber U (1986) Effects of β-agonists on b- and c-waves suggest adrenergic mechanisms in cat retina. Doc Ophthalmol (ISCEV) (in press)
Onoe S, Yoshimura Y, Mori T, Tazawa Y (1985) The effect of beta blocker on rabbit in vivo ERG c-wave. 23rd Symposium ISCEV, Mie, Japan, May 22–25, p 44
Osborne NN (1981) Noradrenaline, a transmitter candidate in the retina. J Neurochem 36: 17–27
Papst N, Demant E, Niemeyer G (1982) Changes in PO2 induced retinal autoregulation in vitro. Graefe's Arch Clin Exp Ophthalmol 219: 6–10
Riegger C (1981) In vitro Versuche in Inkubationsbad zum Studium der Wirkung der vasoaktiven Substanz Serotonin auf die humane Basilararteria. Diploma thesis, Kantonsspital Basel, Departement Forschung, Abteilung Neurobiologie
Schlumpf M, Bruininck A, Lichtensteiger W, Cortes R, Palacios JM, Pazos A (1986) β-Adrenergic binding sites in fetal rat C.N.S. and pineal gland: their relation to other receptor sites. Dev Pharmacol Ther (in press)
Steinberg RH, Linsenmeier RA, Griff ER (1985) Retinal pigment epithelial cell contributions to the electroretinogram and electrooculogram. In: Osborne NN, Chader GJ (eds) Progress in retinal research, vol 4. Pergamon Press, New York, pp 33–66
Weigelin E, Niesel P, von dem Wolks D (1953) Über die Wirkung von Dilatol am intrakraniellen Kreislauf. Ärztl Wochenschr 8: 1171–1172
Winsor T, Hyman C, Knapp FM (1960) The cerebral peripheral and circulatory action of Nylidrin Hydrochloride. Am J Med Sci 239: 594–600
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Presented in part at the 78th Congress of the Swiss Ophthalmological Society, St. Moritz, September 1985
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Niemeyer, G., Cottier, D. & Resch, H. Effects of buphenine (nylidrin) on the perfused mammalian eye. Graefe's Arch Clin Exp Ophthalmol 225, 33–38 (1987). https://doi.org/10.1007/BF02155801
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DOI: https://doi.org/10.1007/BF02155801