Skip to main content

Advertisement

SpringerLink
Log in

Effects of veraguensin and galgravin on osteoclast differentiation and function

  • JAACT Special Issue
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

The dried flower buds of Magnolia sp. are widely used as herbal medicines because of their anti-inflammatory, anti-malarial and anti-platelet activities. Here, we found that veraguensin and galgravin, lignan compounds derived from Magnolia sp., dose-dependently inhibited osteoclast formation in co-cultures of bone marrow cells and osteoblastic cells. These compounds also inhibited receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast differentiation in RAW264.7 cells and bone marrow macrophages. In the RANKL-induced signaling pathway, veraguensin and galgravin reduced p38 phosphorylation and suppressed the expression of c-Fos, a key transcription factor for osteoclastogenesis. Veraguensin and galgravin also inhibited osteoclastic pit formation, which was accompanied by decreased mature osteoclast viability. In conclusion, these results indicate that veraguensin and galgravin can inhibit bone resorption and may offer novel compounds for the development of drugs to treat bone-destructive diseases such as osteoporosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Boyle WJ, Simonet WS, Lacey DL (2003) Osteoclast differentiation and activation. Nature 423:337–342

    Article  CAS  Google Scholar 

  • Chen ZN, Yu PZ, Xu PJ (1993) Anti-platelet activating factor constituents, 2,5-diaryltetrahydrofuran type lignans, from Piper futokadsura Sied. et Zucc. Zhongguo Zhong Yao Za Zhi 18:292–294, 318

    Google Scholar 

  • da Silva Filho AA, Andrade e Silva ML, Carvalho JC, Bastos JK (2004) Evaluation of analgesic and anti-inflammatory activities of Nectandra megapotamica (Lauraceae) in mice and rats. J Pharm Pharmacol 56:1179–1184

    Article  Google Scholar 

  • da Silva Filho AA, Costa ES, Cunha WR, e Silva ML, Nanayakkara NP, Bastos JK (2008) In vitro antileishmanial and antimalarial activities of tetrahydrofuran lignans isolated from Nectandra megapotamica (Lauraceae). Phytother Res 22:1307–1310

  • Feng X (2005) Regulatory roles and molecular signaling of TNF family members in osteoclasts. Gene 25:1–13

    Article  Google Scholar 

  • Harvey AL (2008) Natural products in drug discovery. Drug Discov Today 13:894–901

    Article  CAS  Google Scholar 

  • Hasegawa S, Yonezawa T, Ahn JY, Cha BY, Teruya T, Takami M, Yagasaki K, Nagai K, Woo JT (2010) Honokiol inhibits osteoclast differentiation and function in vitro. Biol Pharm Bull 33:487–492

    Google Scholar 

  • Kim SN, Kim MH, Kim YS, Ryu SY, Min YK, Kim SH (2010) Inhibitory effect of (-)-saucerneol on osteoclast differentiation and bone pit formation. Phytother Res 23:185–191

    Google Scholar 

  • Lacey DL, Timms E, Tan HL, Kelley MJ, Dunstan CR, Burgess T, Elliott R, Colombero A, Elliott G, Scully S, HSU H, Sullivan J, Hawkins N, Davy E, Capparelli C, Eli A, Qian YX, Kaufman S, Sarosi I, Shalhoub V, Senaldi G, Guo J, Delaney J, Boyle WJ (1998) Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell 93:165–176

    Google Scholar 

  • Lam KS (2007) New aspects of natural products in drug discovery. Trends Microbiol 15:279–289

    Article  CAS  Google Scholar 

  • Li X, Udagawa N, Itoh K, Suda K, Murase Y, Nishihara T, Suda T, Takahashi N (2002) p38 MAPK-mediated signals are required for inducing osteoclast differentiation but not for osteoclast function. Endocrinology 143:3105–3113

    Google Scholar 

  • Lim H, Son KH, Bae KH, Hung TM, Kim YS, Kim HP (2009) 5-lipoxygenase-inhibitory constituents from Schizandra fructus and Magnolia flos. Phytother Res 23:1489–1492

  • Rodan GA, Martin TJ (2000) Therapeutic approaches to bone diseases. Science 289:1508–1514

    Article  CAS  Google Scholar 

  • Suda T, Nakamura I, Jimi E, Takahashi N (1997) Regulation of osteoclast function. J Bone Miner Res 12:869–879

    Google Scholar 

  • Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, Martin TJ (1999) Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr Rev 20:345–357

    Google Scholar 

  • Takahashi N, Udagawa N, Suda T (1999) A new member of tumor necrosis factor ligand family, ODF/OPGL/TRANCE/RANKL, regulates osteoclast differentiation and function. Biochem Biophys Res Commun 24:449–455

    Article  Google Scholar 

Download references

Acknowledgments

This study was performed at a laboratory supported by an endowment from ERINA Co., Inc.

Author information

Authors and Affiliations

  1. Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai, Aichi, 487-8501, Japan

    Midori Asai, Yasunori Itakura, Bong-Keun Choi, Motoko Ohnishi & Je-Tae Woo

  2. Research Institute for Biological Functions, Chubu University, 1200 Matsumoto, Kasugai, Aichi, 487-8501, Japan

    Takayuki Yonezawa, Toshiaki Teruya, Byung-Yoon Cha, Motoko Ohnishi & Je-Tae Woo

  3. Section of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Ji-Won Lee & Akira Yamaguchi

Authors
  1. Midori Asai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ji-Won Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yasunori Itakura
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bong-Keun Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takayuki Yonezawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Toshiaki Teruya
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Byung-Yoon Cha
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Motoko Ohnishi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Akira Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Je-Tae Woo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Je-Tae Woo.

Additional information

Midori Asai and Ji-Won Lee contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Asai, M., Lee, JW., Itakura, Y. et al. Effects of veraguensin and galgravin on osteoclast differentiation and function. Cytotechnology 64, 315–322 (2012). https://doi.org/10.1007/s10616-011-9416-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-011-9416-z

Keywords

Search

Navigation