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Determination of saikosaponins a, c, and d in Bupleurum Chinese DC from different areas by capillary zone electrophoresis

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Abstract

A fast capillary zone electrophoresis (CZE) method was developed for the determination and separation of saikosaponins a, c, and d in Chinese herbal extracts of Bupleurum Chinese DC from different areas. Detection at 214 nm with a system containing sodium borate buffer and mono-3-phenylcarbamoyl-β-CD was found to be the most suitable approach for this analysis. Saikosaponins a, c, and d could be easily determined within 8 min. The effect of the concentration of mono-3-phenylcarbamoyl-β-CD, the concentration of the running buffer and buffer pH value on the migration behavior of the saikosaponins is discussed.

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Acknowledgements

This work was supported by the Doctoral Program Foundation of Shandong Natural Science (No. 03BS046).

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Authors and Affiliations

  1. School of Pharmacy, Shandong University, Wen Hua Xi Road 44, Jinan 250012, P. R. China

    Xiuli Lin

  2. School of Chinese Pharmacy, Shandong University of Traditional Chinese Medicine, Jing Shi Road 53, Jinan 250014, P. R. China

    Ling Xue & Huiyun Zhang

  3. School of Chemistry and Chemical Engineering, Shandong University, Shan Da Nan Road 27, Jinan 250100, P. R. China

    Chenfu Zhu

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  1. Xiuli Lin
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  2. Ling Xue
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  3. Huiyun Zhang
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  4. Chenfu Zhu
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Correspondence to Chenfu Zhu.

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Lin, X., Xue, L., Zhang, H. et al. Determination of saikosaponins a, c, and d in Bupleurum Chinese DC from different areas by capillary zone electrophoresis. Anal Bioanal Chem 382, 1610–1615 (2005). https://doi.org/10.1007/s00216-005-3371-5

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  • DOI: https://doi.org/10.1007/s00216-005-3371-5

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