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Antiviral activity of GuiQi polysaccharides against enterovirus 71 in vitro

  • Research Article
  • Published:
Virologica Sinica

Abstract

In this study, we have investigated the antiviral activity of GuiQi polysaccharides (GQP) upon enterovirus 71 (EV71) in vitro. An assay using methyl thiazolyl tetrazolium (MTT), and analyses of cytopathic effects (CPE) were used to examine the antiviral activity of GQP upon Vero cells infected with EV71. The results revealed that GQP at concentrations below 31.2 μg/mL exhibited significant antiviral effects upon EV71 when applied under three different experimental protocols. GQP was most strongly active in preventing the adsorption of EV71 to target cells and in this respect it was significantly more effective than ribavirin. In addition, it was clear that GQP could inhibit viral replication when added to cells 2 h after infection, but if added at the point of infection its effect was weak. GQP is considered to be less toxic than ribavirin, and may warrant further evaluation as a possible agent in the treatment of hand, foot and mouth disease (HFMD).

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Authors and Affiliations

  1. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China

    Xiuying Pu, Hengrui Wang, Wenbo Fan & Shuang Yu

  2. Shanghai Hengrui Pharmaceutical Limited Company, Shanghai, 200245, China

    Yan Li

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  1. Xiuying Pu
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  2. Hengrui Wang
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  3. Yan Li
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  4. Wenbo Fan
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  5. Shuang Yu
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Correspondence to Xiuying Pu.

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Pu, X., Wang, H., Li, Y. et al. Antiviral activity of GuiQi polysaccharides against enterovirus 71 in vitro . Virol. Sin. 28, 352–359 (2013). https://doi.org/10.1007/s12250-013-3376-8

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  • DOI: https://doi.org/10.1007/s12250-013-3376-8

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