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Separation and Purification of Baishouwubenzophenone, 4-Hydroxyacetophenone and 2,4-Dihydroxyacetophenone from Cynanchum auriculatum Royle ex Wight by HSCCC

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Abstract

A preparative high-speed counter-current chromatography method for isolation and purification of three acetophenones, baishouwubenzophenone, 4-hydroxyacetophenone and 2,4-dihydroxyacetophenone from the Chinese medicinal plant Cynanchum auriculatum Royle ex Wight was successfully established. With a two-phase solvent system composed of light petroleum (b.p. 60–90 °C)–ethyl acetate–methanol–water (4:9:6:6, v/v), about 20.2 mg compound 1, 35.0 mg compound 2 and 8.3 mg compound 3, each at over 95% purity as determined by LC, were obtained in one-step elution from 400 mg of the ethanol extract. The structures of these compounds were identified by UV, IR, ESI-MS, 1H NMR and 13C NMR spectroscopy. Among them, compounds 2,4-dihydroxyacetophenone and 4-hydroxyacetophenone were obtained from C. auriculatum for the first time.

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Acknowledgments

Financial support from the Ministry of Science & Technology of China (No. 2005DKA21000) and Shandong Agricultural University (No. 23489) are gratefully acknowledged.

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Authors and Affiliations

  1. College of Agronomy, Shandong Agricultural University, 271018, Taian, China

    Yinshi Sun, Zhengbo Liu, Jianhua Wang & Lixiang Zhu

  2. State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Taian, China

    Yinshi Sun & Jianhua Wang

  3. School of Pharmacy, Shandong University, 250012, Jinan, China

    Lan Xiang

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  1. Yinshi Sun
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  2. Zhengbo Liu
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  3. Jianhua Wang
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  4. Lan Xiang
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  5. Lixiang Zhu
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Corresponding author

Correspondence to Jianhua Wang.

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Sun, Y., Liu, Z., Wang, J. et al. Separation and Purification of Baishouwubenzophenone, 4-Hydroxyacetophenone and 2,4-Dihydroxyacetophenone from Cynanchum auriculatum Royle ex Wight by HSCCC. Chroma 70, 1–6 (2009). https://doi.org/10.1365/s10337-009-1107-y

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