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Mechano growth factor E peptide promotes osteoblasts proliferation and bone-defect healing in rabbits

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Abstract

To assess the potential efficacy of mechano growth factor (MGF) for bone injury, we firstly investigated the effects of growth factors, including MGF, its E peptide (a short 24-amino acid C-terminal peptide, MGF-Ct24E), and insulin-like growth factor 1(IGF-1) on MC3T3-E1 osteoblast-like cell proliferation. MGF-Ct24E had the highest pro-proliferation activity among three growth factors, which was 1.4 times greater than that of IGF-1. Moreover, MGF-Ct24E promoted cell proliferation by inducing cell cycle arrest in the S and G2/M phase of the cell cycle, but also mainly by the activation of the MAPK-Erk1/2 pathway. In vivo, a 5-mm segmental bone defect in the radius of 27 rabbits was treated with MGF-Ct24E by two doses (28.5 and 57 μg /kg body weight) vs. non-growth factor injection for five consecutive days postoperatively. The cumulative rate of radiographically healed defects and histological scores of bone defect-healing revealed a statistical difference between high-dose treatment and non treatment (p < 0.01), which showed the treatment promoted defect healing. This report is the first to demonstrate that MGF-Ct24E possesses positive effects on osteoblast proliferation and bone-defect healing, suggesting a new strategy in fracture healing.

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Acknowledgements

This work was supported by the National Nature Science Foundation of China (1032012, 30870609); Natural Science Foundation of Chongqing (CSTC2009BB4382CSTC2010BB5225); Foundation for Science & Technology Research Project of Chongqing (CSTC 2009AB517) and Project Foundation of Chongqing Municipal Education Committee (KJ091415).

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Authors and Affiliations

  1. Ministry of Education, Key Laboratory of Biorheological Science and Technology, Research Center of Bioinspired Materials, Science and Engineering of National “985 Project program” of China, Chongqing University, Chongqing, China

    Moyuan Deng, Bingbing Zhang, Yuxiao Li, Fuchun Lin & Yuanliang Wang

  2. Department of Orthopaedics, Daping Hospital and Research Institute of Surgery, Chongqing, China

    Ke Wang, Feng Liu, Jianhua Zhao & Peng Liu

  3. Department of Pathology, Daping Hospital, Third Military Medical University, Chongqing, China

    Hualiang Xiao

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  1. Moyuan Deng
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  2. Bingbing Zhang
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  3. Ke Wang
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  4. Feng Liu
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  5. Hualiang Xiao
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  6. Jianhua Zhao
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  7. Peng Liu
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  8. Yuxiao Li
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  9. Fuchun Lin
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  10. Yuanliang Wang
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Correspondence to Yuanliang Wang.

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Deng, M., Zhang, B., Wang, K. et al. Mechano growth factor E peptide promotes osteoblasts proliferation and bone-defect healing in rabbits. International Orthopaedics (SICOT) 35, 1099–1106 (2011). https://doi.org/10.1007/s00264-010-1141-2

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  • DOI: https://doi.org/10.1007/s00264-010-1141-2

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