Skip to main content
SpringerLink
Log in

Oxidative stress in the mollusk Echinolittorina peruviana (Gasteropoda: Littorinidae, Lamarck, 1822) and trace metals in coastal sectors with mining activity

  • Published:
Ecotoxicology Aims and scope Submit manuscript

Abstract

The aim of the study was to evaluate the effect of coastal waters of sites with mining activity in Echinolittorina peruviana, through oxidative stress biomarkers and heavy metals determination both in water and in tissue. Organisms were collected in the intertidal zone in areas with and without mining activity. Metal concentrations in the water and tissues, and also, the following biomarkers of oxidative stress: antioxidant enzyme activity, superoxide dismutase and catalase, non-enzymatic oxidative capacity (TRAP), oxidative damage to proteins (carbonyls) and TBARS, were measured The concentrations of accumulated metals had the following order Fe > Cu > Cd > Zn > Cr > Mo > As; the highest concentrations of metals in water and tissues were found in Caleta Palito and Chañaral. Results suggest that the coastal waters with mining activity and greatest concentrations of copper and iron induced the greater antioxidant response and oxidative damage to lipids in E. peruviana.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Aebi H (1984) Catalase in vitro. Method Enzymol 105:121–126

    Article  CAS  Google Scholar 

  • Almeida E, Bainy A, Medeiros M, Mascio P (2003) Effects of trace metal and exposure to air on serotonin and dopamine levels in tissues of the mussel Perna perna. Mar Pollut Bull 46:1485–1490

    Article  CAS  Google Scholar 

  • Baqueiro-Cárdenas E, Borabe L, Goldaracena-Islas C, Rodríguez-Navarro J (2007) Mollusks and pollution. A review. Rev Mex Biodivers 78:1S–7S

    Google Scholar 

  • Baumann H, Morrison L, Stengel D (2009) Metal accumulation and toxicity measured by PAM-chlorophyll fluorescence in seven species of marine macroalgae. Ecotoxicol Environ Saf 72:1063–1075

    Article  CAS  Google Scholar 

  • Boese B, Lee H (1992) Synthesis of methods to predict bioaccumulation of sediment pollutants. Environmental Protection Agency. Environmental Research Laboratory. Narragansett RI. ERL-N No. N232. U.S

  • Box A, Sureda A, Galgani F, Pons A, Deudero S (2007) Assessment of environmental pollution at Balearic Islands applying oxidative stress biomarkers in the mussel Mytilus galloprovincialis. Comp Biochem Physiol 146(C):531–539

    CAS  Google Scholar 

  • Carvalho-Neta R, Abreu-Silva A (2010) Sciades herzbergii oxidative stress biomarkers: an in situ study of an estuarine ecosystem (São Marcos’ Bay, Maranhão, Brazil). Braz J Oceanogr 58:11–17

    Article  Google Scholar 

  • Castillo V, Brown D (2008) Microscopic anatomy of the male reproductive system in Echinolittorina peruviana (Mollusca: Caenogastropoda). Int J Morphol 26(2):423–432

    Article  Google Scholar 

  • Cataldo J, Hidalgo M, Neaman A, Gaete H (2011) Use of molecular biomarkers in Eisenia foetida to assess copper toxicity in agricultural soils affected by mining activities. J Soil Sci Plant Nutr 11(3):57–70

    Google Scholar 

  • Comín F, Menéndez M, Romero J, Hernández O, Martínez M, Chacón A (1999) Indicadores ecológicos y herramientas para la gestión de ecosistemas acuáticos en la zona costera. Limnetica 16:61–68

    Google Scholar 

  • Conti M, Stripeikis J, Iacobucci M, Cucina D, Cecchetti G, Tudino M (2006) Trace metals in molluscs from the Beagle Channel (Argentina): a preliminary study. WIT Trans Ecol Environ 99:473–483

    Article  Google Scholar 

  • Conti M, Stripeikis J, Finoia M, Tudino M (2012) Baseline trace metals in gastropod mollusks from the Beagle Channel, Tierra del Fuego (Patagonia, Argentina). Ecotoxicology 21:1112–1125

    Article  CAS  Google Scholar 

  • Contreras L, Dennett G, González A, Vergara E, Medina C, Correa J, Moenne A (2011) Identification of copper-induced genes in the marine alga Ulva compressa (Chlorophyta). Mar Biotechnol 13:544–556

    Article  Google Scholar 

  • Dahms H, Dobretsov S, Lee J (2011) Effects of UV radiation on marine ectotherms in polar regions. Comp Biochem Physiol 153(C):363–371

    Google Scholar 

  • Damásio J, Navarro-Ortega A, Tauler R, Lacorte S, Barceló D, Soares A, López M, Riva M, Barata C (2010) Identifying major pesticides affecting bivalve species exposed to agricultural pollution using multi-biomarker and multivariate methods. Ecotoxicology 19:1084–1094

    Article  Google Scholar 

  • Duffus J (2002) Heavy metals—a meaningless term. Pure Appl Chem 74:763

    Article  Google Scholar 

  • El-Gendy K, Radwan M, Gad A (2009) In vivo evaluation of oxidative stress biomarkers in the land snail, Theba pisana exposed to copper-based pesticides. Chemosphere 77:339–344

    Article  CAS  Google Scholar 

  • Esterbauer K, Cheeseman H, Dianzani M, Poli G, Slater T (1982) Separation and characterization of the aldehydic products of lipid peroxidation stimulated by ADP-Fe2+ in rat liver microsomes. Biochem J 208(1):129–140

    CAS  Google Scholar 

  • Fang T, Hwang J, Hsiao S (2006) Trace metals in seawater and copepods in the ocean outfall area off northern Taiwan coast. Mar Environ Res 61:224

    Article  CAS  Google Scholar 

  • Fridovich I (1995) Superoxide radical and superoxide dismutases. Annu Rev Biochem 64:97–112

    Article  CAS  Google Scholar 

  • Funes V, Alhama J, Navas J, López-Barea J, Peinado J (2006) Ecotoxicological effects of metal pollution in two mollusc species from the Spanish South Atlantic littoral. Environ Pollut 139:214–223

    Article  CAS  Google Scholar 

  • Gaete H, Hidalgo M, Neaman A, Ávila G (2010) Evaluación de la toxicidad de cobre en suelos a través de biomarcadores de estrés oxidativo en Eisenia foetida. Quim Nova 33(3):566–570

    Article  CAS  Google Scholar 

  • Guzmán N, Saá S, Ortlieb L (1998) Catálogo descriptivo de los moluscos litorales (gastropoda y pelecypoda) de la zona de Antofagasta, 23°s (Chile). Estud Oceanol 17:17–86

    Google Scholar 

  • Halliwell B (2006) Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol 141:312–322

    Article  CAS  Google Scholar 

  • Halliwell B, Gutteridge J (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219:1–14

    CAS  Google Scholar 

  • Halliwell B, Gutteridge J (1989) Free radical in biology and medicine. Clarendon, Oxford, pp 1–142

    Google Scholar 

  • Halliwell B, Gutteridge J (1990) The antioxidants of human extracellular fluids. Arch Biochem Biophys 280:1–8

    Article  CAS  Google Scholar 

  • Halliwell B, Gutteridge J (2006) Free radicals in biology and medicine, 4th edn. Clarendon Press, Oxford

    Google Scholar 

  • Hermes-Lima M (2004) Oxygen in biology and biochemistry: role of free radicals. In: Storey KB (ed) Functional metabolism: regulation and adaptation. Wiley, New York, pp 319–368

  • Hidalgo M, Fernández E, Cabello A, Rivas C, Fontecilla F, Morales L, Aguirre A, Cabrera E (2006) Evaluation of the antioxidant response of Chiton granosus Frembly, 1928 (Mollusca: Polyplacophora) to oxidative pollutants. Rev Biol Mar Oceanogr 41(2):155–165

    Article  Google Scholar 

  • Jakimska A, Konieczka P, Skóra K, Namieśnik J (2011) Bioaccumulation of metals in tissues of marine animals, Part II: metal concentrations in animal tissues. Pol J Environ Stud 20(5):1127–1146

    CAS  Google Scholar 

  • Je J, Belan T, Levings C, Koo B (2004) Changes in benthic communities along a presumed pollution gradient in vancouver harbour. Mar Environ Res 57:121–135.

  • Knigge T, Mann N, Parveen Z, Perry C, Gernhofer M, Triebskorn R, Kohler H, Connock M (2002) Mannosomes: a molluscan intracellular tubular membrane system related to heavy metal stress? Comp Biochem Physiol 131(1):259–269

    Google Scholar 

  • Lane N (2002) Oxygen, the molecule that made the world. Oxford University Press, Oxford

    Google Scholar 

  • Lee M, Correa J (2005) Effects of copper mine tailings disposal on littoral meiofaunal assemblages in the Atacama region of northern Chile. Mar Environ Res 59:1–18

    Article  CAS  Google Scholar 

  • Lee M, Correa J, Zhang H (2002) Effective metal concentrations in porewater and seawater labile metal concentrations associated with copper mine tailings disposal into the coastal waters of the Atacama region of northern Chile. Mar Pollut Bull 44:956–976

    Article  CAS  Google Scholar 

  • Lewis A (1990) The biological importance of copper. A literature review. Final report INC Aproject No

  • Lowry O, Rosebrough N, Farr A, Randall R (1951) Protein measurement with the Folin-Phenol reagents. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Lushchak V, Bagnyukova T (2006) Temperature increase results in oxidative stress in gold fish tissues. 2. Antioxidant and associated enzymes. Comp Biochem Physiol 143(c):36–41

  • Morriconi E (1999) Reproductive biology of the limpet Nacella (P.) deaurata (Gmelin, 1791) in Bahia Lapataia (Beagle Channel). Sci Mar 63:417–426

    Article  Google Scholar 

  • Nicotri M (1977) Grazing effects of four marine intertidal herbivores on the microflora. Ecology 58:1020–1032

    Article  Google Scholar 

  • Nowakowska A, Swiderska-Kolacz G, Rogalska J, Caputa M (2009) Antioxidants and oxidative stress in Helix pomatia snails during estivation. Comp Biochem Physiol 150(1):481–486

    Google Scholar 

  • Palamanda J, Kehrer J (1992) Inhibition of protein carbonyl formation and lipid peroxidation by glutathione in rat liver microsomes. Arch Biochem Biophys 293:103–109

    Article  CAS  Google Scholar 

  • Pan L, Ren J, Zheng D (2009) Effects of benzo(α)pyrene exposure on the antioxidant enzyme activity of scallop Chlamys farreri. Chin J Ooceanol Lim 27(1):43–53

    Article  CAS  Google Scholar 

  • Radwan M, El-Gendy K, Gad A (2010) Biomarkers of oxidative stress in the land snail, Theba pisana for assessing ecotoxicological effects of urban metal pollution. Chemosphere 79:40–46

    Article  CAS  Google Scholar 

  • Rodríguez J, Menéndez J, Trujillo Y (2001) Radicales libres en la biomedicina y estrés oxidativo. Rev Cuba Med Mil 30(1):36–44

    Google Scholar 

  • Rojas J, Fariña J, Soto R, Bozinovic F (2000) Geographic variability in thermal tolerance and water economy of the intertidal gastropod Nodilittorina peruviana. (Gastropoda: Littorinidae, Lamarck, 1822). Rev Chil Hist Nat 73:543–552

    Article  Google Scholar 

  • Romay C, Pascual C, Lissi E (1996) The reaction between ABTS radical cation and antioxidants and its use to evaluate the antioxidant status of serum samples. Braz J Med Biol 29:175–183

    CAS  Google Scholar 

  • Sáez C, Lobos G, Macaya E, Oliva D, Quiroz W, Brown MT (2012) Variation in patterns of metal accumulation in thallus parts of Lessonia trabeculata (Laminariales; Phaeophyceae): implications for biomonitoring. PLoS ONE 7(11):1–10

    Article  Google Scholar 

  • Verlecar X, Jena K, Chainy G (2008) Seasonal variation of oxidative biomarkers in gills and digestive gland of green-lipped mussel Perna viridis from Arabian Sea. Estuar Coast Shelf Sci 76:745–752

    Article  Google Scholar 

  • Viarengo A, Burlando B, Giordana A, Bolognesi C, Gabrielides GP (2000) Networking and expert system analysis: next frontier in biomonitoring. Mar Environ Res 49:483–486

    Article  CAS  Google Scholar 

  • Wang C, Zhao Y, Zheng R (2006) Effects of tributyltin, benzo[a]pyrene, and their mixture on antioxidant defense systems in Sebastiscus marmoratus. Ecotoxicol Environ Saf 65(3):381–387

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by the Project No. 28/2009 of the Dirección de Investigación of the Universidad de Valparaíso.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Laboratorio de Investigación—Estrés Oxidativo, Facultad de Medicina, Universidad de Valparaíso, Av. Brasil, 1560, Valparaíso, Chile

    C. Jara

  2. Facultad de Ciencias, Instituto de Ciencias Ambientales, Universidad de Valparaíso, Av. Gran Bretaña, 1111, Playa Ancha, Valparaíso, Chile

    H. Gaete

  3. Centro de Investigación y Gestión de Recursos Naturales (CIGREN), Universidad de Valparaíso, Av. Gran Bretaña, 1111, Playa Ancha, Valparaíso, Chile

    H. Gaete & G. Lobos

  4. Facultad de Ciencias, Instituto de Química y Bioquímica, Universidad de Valparaíso, Av. Gran Bretaña, 1111, Playa Ancha, Valparaíso, Chile

    G. Lobos & M. E. Hidalgo

Authors
  1. C. Jara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Gaete
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Lobos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. E. Hidalgo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. Gaete.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jara, C., Gaete, H., Lobos, G. et al. Oxidative stress in the mollusk Echinolittorina peruviana (Gasteropoda: Littorinidae, Lamarck, 1822) and trace metals in coastal sectors with mining activity. Ecotoxicology 23, 1099–1108 (2014). https://doi.org/10.1007/s10646-014-1253-3

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10646-014-1253-3

Keywords

Search

Navigation