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Hydrogen peroxide detoxifying enzymes show different activity patterns in host and non-host plant interactions with Magnaporthe oryzae Triticum pathotype

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Abstract

Wheat blast caused by the hemibiotroph fungal pathogen Magnaporthe oryzae Triticum (MoT) pathotype is a destructive disease of wheat in South America, Bangladesh and Zambia. This study aimed to determine and compare the activities of antioxidant enzymes in susceptible (wheat, maize, barley and swamp rice grass) and resistant (rice) plants when interacting with MoT. The activities of reactive oxygen species-detoxifying enzymes; catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX), glutathione S-transferase (GST), peroxidase (POX) were increased in all plants in response to MoT inoculation with a few exceptions. Interestingly, an early and very high activity of CAT was observed within 24 h after inoculation in wheat, barley, maize and swamp rice grass with lower H2O2 concentration. In contrast, an early and high accumulation of H2O2 was observed in rice at 48 hai with little CAT activity only at a later stage of MoT inoculation. The activities of APX, GST and POD were also high at an early stage of infection in rice. However, these enzymes activities were very high at a later stage in wheat, barley, maize and swamp rice grass. The activity of GPX gradually decreased with the increase of time in rice. Taken together, our results suggest that late and early inductions of most of the antioxidant enzyme activities occurs in susceptible and resistant plants, respectively. This study demonstrates some insights into physiological responses of host and non-host plants when interacting with the devastating wheat blast fungus MoT, which could be useful for developing blast resistant wheat.

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Acknowledgements

This work was supported by Grants from the Krishi Gobeshona Foundation (KGF), Bangladesh Project No. TF50-C/17 and the IAEA/FAO CRP code: D23032.

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  1. Dipali Rani Gupta and Sanjida Khanom have contributed equally to this work.

Authors and Affiliations

  1. Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh

    Dipali Rani Gupta, Sanjida Khanom, Musrat Zahan Surovy, Nur Uddin Mahmud, Md. Robyul Islam, Ashifur Rahman Shawon & Tofazzal Islam

  2. Plant Breeding Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

    Md. Motiar Rohman

  3. Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

    Mirza Hasanuzzaman

  4. Agriculture and Natural Resources, Extension Service, West Virginia University, Morgantown, WV, 26506, USA

    Mahfuzur Rahman

  5. Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza, 12619, Egypt

    Kamel A. Abd-Elsalam

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  1. Dipali Rani Gupta
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Contributions

TI Conceptualized and supervised. DRG, SK, MZS, MRI and MMR conducted the experiments. DRG, NUM, ARS and MH analyzed the data. DRG prepared the original draft. TI, MH, KAA, MR and DRG edited the manuscript. All authors have read and approved the manuscript.

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Correspondence to Tofazzal Islam.

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Gupta, D.R., Khanom, S., Rohman, M.M. et al. Hydrogen peroxide detoxifying enzymes show different activity patterns in host and non-host plant interactions with Magnaporthe oryzae Triticum pathotype. Physiol Mol Biol Plants 27, 2127–2139 (2021). https://doi.org/10.1007/s12298-021-01057-4

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