Abstract
A sensitive modification of the ninhydrin-CO2 method involving the gas chromatographic determination of the total protein and amino acid content of sediment is described. The method gives a linear response over the amino acid concentration range 10−5 M to 4 × 10−2 M. It can be used for whole sediment, hydrolysates and interstitial water. The performance of the method is compared with the fluorescamine method for primary amines.
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Simon, B.M., Jones, J.G. Gas chromatographic determination of total amino acids and protein in sediments using the ninhydrin-CO2 reaction. Hydrobiologia 97, 81–86 (1982). https://doi.org/10.1007/BF00014961
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DOI: https://doi.org/10.1007/BF00014961