Abstract
The replication origin of the 9-kb rDNA repeat size class of pea (Pisum sativum cv. Alaska) was identified by benzoylated naphthoylated DEAE-cellulose column chromatography and Southern blotting procedures. The origin is located at or near a 0.19-kb EcoR I fragment in the non-transcribed spacer region between the 25S and 18S rRNA genes. Identification of the origin was based on three criteria: (i) an enrichment of the 0.19-kb fragment in replicating rDNA from asynchronously dividing root meristematic cells, (ii) the scarcity of the 0.19-kb fragment in rDNA from non-dividing carbohydrate starved cells, and (iii) a 60-min periodic enrichment of the 0.19-kb fragment in replicating rDNA that temporally coincides with the sequential initiation of replication of replicon families in synchronized pea root cells.
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Van't Hof, J., Hernández, P., Bjerknes, C.A. et al. Location of the replication origin in the 9-kb repeat size class of rDNA in pea (Pisum sativum). Plant Mol Biol 9, 87–95 (1987). https://doi.org/10.1007/BF00015641
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DOI: https://doi.org/10.1007/BF00015641