Abstract
A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein could be synthesized in E. coli. The properties of this PEPC protein clearly resembled those of typical C3 plant enzymes.
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References
Andreo, CS, Gonzales, DH, Iglesias, AA: Higher plant phosphoenolpyruvate carboxylase. FEBS Lett 213: 1–8 (1987).
Anraku, Y: The reduction and restoration of galactose transport in osmotically shocked cells of Escherichia coli. J Biol Chem 242: 793–800 (1967).
Bakrim, N, Echevarria, C, Cretin, C, Arrio-Dupont, M, Pierre, JN, Vidal, J, Chollet, R, Gadal, P: Regulatory phosphorylation of Sorghum leaf phosphoenolpyruvate carboxylase: identification of the protein-serine kinase and some elements of the signal transduction cascade. Eur J Biochem 204: 821–830 (1992).
Bradford, M: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–254 (1976).
Cretin, C, Bakrim, N, Keryer, E, Santi, S, Lepiniec, L, Vidal, J, Gadal, P: Production in Escherichia coli of active Sorghum phosphoenolpyruvate carboxylase which can be phosphorylated. Plant Mol Biol 17: 83–88 (1991).
Cushman, JC, Bohnert, HJ: Nucleotide sequence of the Ppc2 gene encoding a housekeeping isoform of phosphoenolpyruvate carboxylase from Mesembryanthemum crystallinum. Nucl Acids Res 17: 6743–6744 (1989).
Denecke M: Reinigung und Charakterisierung der stomatären Phosphoenolpyruvat-Carboxylase aus Vicia faba L. Entwicklung eines geeigneten Verfahrens zur Herstellung monoklonaler Antikörper. Dissertation, University of Bonn, FRG (1991).
Eikmanns, BJ, Follettie, MT, Griot, MU, Sinskey, AJ: The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucloetide sequence, and expression. Mol Gen Genet 218: 330–339 (1989).
Hermans, J, Westhoff, P: Homologous genes for the C4 isoforms of phosphoenolpyruvate carboxylase in a C3 and a C4 Flaveria species. Mol Gen Genet 234: 275–284 (1992).
Izui, K, Ishijima, S, Yamaguchi, Y, Katagiri, F, Murata, T, Shigesada, K, Sugiyama, T, Katsuki, F: Cloning and sequence analysis of cDNA encoding active phosphoenolpyruvate carboxylase of the C4-pathway from maize. Nucl Acids Res 14: 1615–1628 (1986).
Jiao, JA, Vidal, J, Echevarria, C, Chollet, R: In vivo regulatory phosphorylation site in C4 leaf phosphoenolpyruvate carboxylase from maize and sorghum. Plant Physiol 96: 297–301 (1991).
Koizumi, N, Sato, F, Terano, Y, Yamada, Y: Sequence analysis of cDNA encoding phosphoenolpyruvate carboxylase from cultured tobacco cells. Plant Mol Biol 17: 535–539 (1991).
Latzko, E, Kelly, GJ: The many-faceted function of phosphoenolpyruvate carboxylase in C3 plants. Physiol Vég 21: 805–815 (1983).
Lepiniec, L, Keryer, E, Philippe, H, Gadal, P, Cretin, C: Sorghum phosphoenolpyruvate carboxylase gene family: structure, function and molecular evolution. Plant Mol Biol 21: 487–502 (1993).
O'Leary, MH: Phosphoenolpyruvate carboxylase: an enzymologist's view. Annu Rev Plant Physiol 33: 297–315 (1982).
Poetsch, W, Hermans, J, Westhoff, P: Multiple cDNAs of phosphoenolpyruvate carboxylase in the C4 dicot Flaveria trinervia. FEBS Lett 292: 133–136 (1991).
Rickers, J, Cushman, JC, Michalowski, CB, Schmitt, JM, Bohnert, HJ: Expression of the CAM form of phosphoenolpyruvate carboxylase and nucleotide sequence of a full length cDNA from Mesembryanthemum crystallinum Mol Gen Genet 215: 447–454 (1989).
Sabe, H, Miwa, T, Kodaki, T, Izui, K, Hiraga, S, Katsuki, H: Molecular cloning of the phosphoenolpyruvate carboxylase gene, ppc, of Escherichia coli. Gene 31: 279–283 (1984).
Schnabl, H, Denecke, M, Schulz, M: In vitro and in vivo phosphorylation of stomatal phosphoenolpyruvate carboxylase from Vicia faba L. Bot Acta 105: 367–369 (1992).
Sugimoto, T, Kawasaki, T, Kato, T, Whittier, RF, Shibata, D, Kawamura, Y: cDNA sequence and expression of a phosphoenolpyruvate carboxylase gene from soybean. Plant Mol Biol 20: 743–747 (1992).
Ting, IP, Osmond, CB: Photosynthetic phosphoenolpyruvate carboxylases. Characteristics of alloenzymes from leaves of C3 and C4 plants. Plant Physiol 51: 439–447 (1973).
Yanagisawa, S, Izui, K, Yamaguchi, Y, Shigesada, K, Katsuki, H: Further analysis of cDNA clones for maize phosphoenolpyruvate carboxylase involved in C4 photosynthesis. FEBS Lett 229: 107–110 (1988).
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Merkelbach, S., Gehlen, J., Denecke, M. et al. Cloning, sequence analysis and expression of a cDNA encoding active phosphoenolpyruvate carboxylase of the C3 plant Solanum tuberosum . Plant Mol Biol 23, 881–888 (1993). https://doi.org/10.1007/BF00021542
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DOI: https://doi.org/10.1007/BF00021542