Summary
Cultivar Peking has been extensively used as a source of resistance to Race 3 and Race 5 of soybean cyst nematode, Heterodera glycines I., and Peking genes for resistance are present in a wide range of resistant soybean cultivars. Peking is also used as a host differential in the soybean cyst nematode race classification system. Thirteen Peking lines maintained in the USDA Soybean Germplasm Collection and in several breeding programs were surveyed using RFLP and RAPD markers for genetic characterization. Based on the molecular diversity combined with reaction to soybean cyst nematode, Peking genotypes from a common original source were identified. Peking lines PI 297543 (introduction from Hungary), and PI 438496A, PI 438496B and PI 438496C (introductions from Russia) represented unrelated germplasms. Identified molecular polymorphism can be used to validate the genetic purity of Peking lines used as host differentials for soybean cyst nematode classification system as well as utilization of an individual germplasm line in genetic-breeding programs.
Similar content being viewed by others
References
Anand, S.C., K.M. Gallo, I.A. Baker & E.E. Hartwig, 1988. Soybean plant introductions with resistance to race 4 and 5 of soybean cyst nematode. Crop Sci. 28: 563–564.
Anand, S.C. & G.O. Meyers, 1992. Genetic relationship between PI 437654 and other sources of resistance to soybean cyst nematode races 3, 4, and 14. Soy. Genet. Newsl. 119: 49–55.
Anand, S.C. & A.P. Rao-Arelli, 1989. Genetic analyses of soybean genotypes resistant to soybean cyst nematode Race 5. Crop Sci. 29: 1181–1184.
Anand, S.C., A.J. Wrather & C.R. Shumway, 1985. Soybean genotypes with resistance to races of soybean cyst nematode. Crop Sci. 25: 1073–1075.
Bernard, R.L., G.A. Juvik & J.L. Nelson, 1987. USDA Soybean Germplasm Collection Inventory. Int. Soy. Series. Number 30. 1: 1–73.
Bernard, R.L., G.A. Juvik & R.L. Nelson, 1989. USDA Soybean Germplasm Collection Inventory. Int. Soy. Series. Number 31. 2: 1–203.
Caldwell, B.E., C.A. Brim & J.P. Ross, 1960. Inheritance of resistance of soybeans to cyst nematode, Heterodera glycines. Agron. J. 52: 635–636.
Epps, J.M. & E.E. Hartwig, 1972. Reaction of soybean varieties and strains to race 4 of the soybean cyst nematode. J. of Nematol. 4: 222.
Feinberg, A.P. & B. Vogelstein, 1983. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137: 266–267.
Fisher, R.A., 1950. Statistical methods for research workers. In: F.E. Crew (Ed.). Hafner Publishing Company Inc. NY.
Hancock, J.A., F.G. Hancock, C.E. Caviness & R.D. Riggs, 1987. Genetics of resistance to ‘Race X’ of soybean cyst nematode. Crop Sci. 27: 704–707.
Hartwig, E.E., 1985. Breeding productive soybeans with resistance to the soybean cyst nematode. In: R. Shibles (Ed.). Proc. World Soybean Res. Conf. III, Ames, IA, USA. 12–17 Aug. 1984. pp. 394–399. Westview Press, Inc., Boulder, Co.
Johnson, R.A. & D.W. Wichern, 1982. Analysis of covariance structure. In: K.M. Lafferty (Ed.). Applied multivariate statistical analysis. Prentice-Hall, Inc. Englewood Cliffs, NJ.
Keim, P., B.W. Diers, T.C. Olson & R.C. Shoemaker, 1990a. RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics 126: 735–742.
Keim, P., B.W. Diers & R.C. Shoemaker, 1990b. Genetic analysis of soybean hard seededness with molecular markers. Theor. Appl. Genet. 79: 465–469.
Keim, P., T. Olson & R.C. Shoemaker, 1988. A rapid protocol for isolating soybean DNA. Soy. Genet. Newsl. 15: 151–152.
Keim, P. & R.C. Shoemaker, 1988. Construction of a random recombinant DNA library that is primarily single copy sequence. Soy. Genet. Newsl. 15: 147–148.
Lohnes, D.G. & R.L. Bernard, 1991. Ancestry of US/Canadian commercial cultivars developed by public institutions. Soy. Genet. Newsl. 18: 243–255.
Luedders, V.D., 1987. A recessive gene for a cyst phenotype in soybean. Crop Sci. 27: 604–606.
Matson, A.L. & L.F. Williams, 1965. Evidence of a fourth gene for resistance to the soybean cyst nematode. Crop Sci. 5: 477.
Nickell, A., T. Olson, L. Lorenzen, S. Lonning, K. Polzin & R. Shoemaker, 1992. Saturating the soybean molecular map. Proc. of the 4th Biennial Conf. on Molecular and Cellular Biology of the Soybean. pp. 33. Ames, IA.
Noel, G.R., 1992. History, distribution, and economics. In: R.D. Riggs & J.A. Wrather (Eds.). Biology and managements of the soybean cyst nematode. APS Press, St Paul, MN.
Rao-Arelli, A.P. & S.C. Anand, 1988. Genetic relationship among soybean plant introductions for resistance to race 3 of soybean cyst nematode. Crop Sci. 28: 650–652.
Rao-Arelli, A.P., S.C. Anand & G.O. Myers, 1989. Partial dominance of susceptibility in soybean to soybean cyst nematode races 3, 4, and 5. Crop Sci. 29: 1562–1564.
Rao-Arelli, A.P., S.C. Anand & J.A. Wrather, 1991a. Genetic relationship of resistance to soybean cyst nematode races 3 and 5 in soybean accessions. pp. 113. Agron. Abstr. Amer. Soc. of Agron., Madison, WI.
Rao-Arelli, A.P., S.C. Anand & J.A. Wrather, 1992. Soybean resistance to soybean cyst nematode race 3 is conditioned by an additional dominant gene. Crop Sci. 32: 862–864.
Rao-Arelli, A.P., K.W. Matson & S.C. Anand, 1991b. A rapid method for inoculating soybean seedlings with Heterodera glycines. Plant Dis. 75: 594–596.
Rao-Arelli, A.P., J.A. Wrather & S.C. Anand, 1992. Genetic diversity among isolates of Heterodera glycines and sources of resistance in soybeans. Plant Dis. 76: 894–896.
Riggs, R.D. & D.P. Schmitt, 1987. Nematodes. In: J.R. Wilcox (Ed.). Soybean: Improvement, Production, and Uses, pp. 757–778. Amer. Soc. of Agron., Madison, Wisconsin, USA.
Riggs, R.D. & D.P. Schmitt, 1988. Complete characterization of the race scheme for Heterodera glycines. J. of Nematol. 20: 392–395.
Rogers, S.O. & A.G. Bendich, 1985. Extraction of DNA from milligram amount of fresh, herbarium and mummified plant tissue. Plant Mol. Biol. 5: 69–76.
Ross, J.P. & C.A. Brim, 1957. Resistance of soybeans to the soybean cyst nematode as determined by a double-row method. Plant Dis. Rep. 41: 923–924.
SAS Institute, 1985. User's guide: Statistics 5th ed. SAS Inst., Cary, NC.
Southern, E., 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517.
Sugiyama, S. & H. Katsumi, 1966. A resistant gene of soybeans to the soybean cyst nematode observed from the cross between Peking and Japanese varieties. Japan J. Breed 16: 83–86.
Triantaphyllou, A.C., 1975. Genetic structure of races of Heterodera glycines and inheritance of ability to reproduce on resistance soybeans. J. of Nematol. 7: 356–364.
Winstead, W.W., C.B. Skotland & J.W. Sasser, 1955. Soybean cyst nematode in North Carolina. Plant Dis. Rep. 39: 9–11.
Young, L.D., 1990. Soybean germplasm evaluated for resistance to Races 3, 5, and 14 of soybean cyst nematode. Crop Sci. 30: 735–736.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Skorupska, H.T., Choi, I.S., Rao-Arelli, A.P. et al. Resistance to soybean cyst nematode and molecular polymorphism in various sources of Peking soybean. Euphytica 75, 63–70 (1994). https://doi.org/10.1007/BF00024532
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00024532