Abstract
A new cold-regulated (COR) gene, msa CIC, was isolated by differential screening of a cDNA library from cold-acclimated crowns of alfalfa (Medicago sativa L. cv. Apica). Transcripts of msa CIC were not detectable in unacclimated alfalfa and accumulated to higher levels in cold-acclimated plants of the cold-tolerant cv. Apica than in those of the cold-sensitive cv. CUF-101. The DNA sequence analysis of a full-length cDNA clone revealed that msa CIC encodes for a putative protein (MSACIC) of 166 amino acids with distinct proline-rich and hydrophobic domains. Protein sequence comparisons indicated that MSACIC is similar to a group of bimodular proteins that are developmentally regulated in other plant species.
This is a preview of subscription content, log in via an institution to check access.
References
Aleith F, Richter G: Gene expression during induction of somatic embryogenesis in carrot cell suspensions. Planta 183: 17–24 (1990).
Castonguay Y, Nadeau P, Laberge S: Freezing tolerance and alteration of translatable mRNAs in alfalfa (Medicago sativa L.) hardened at subzero temperatures. Plant Cell Physiol 34: 31–38 (1993).
Fourney RM, Miyakoshi J, Day RS, Paterson MC: Northern blotting: efficient RNA staining and transfer. Focus 10: 5–7 (1988).
Guo W, Ward RW, Thomashow MF: Characterization of a cold-regulated wheat gene related to Arabidopsis cor47. Plant Physiol 100: 915–922 (1992).
Houde M, Danyluk J, Laliberté J-F, Rassart E, Dhindsa RS, Sarhan F: Cloning, characterization, and expression of a cDNA encoding a 50-kilodalton protein specifically induced by cold acclimation in wheat. Plant Physiol 99: 1381–1387 (1992).
John I, Wang H, Held BM, Wurtele ES, Colbert JT: An mRNA that specifically accumulates in maize roots delineates a novel subset of developing cortical cells. Plant Mol Biol 20: 821–831 (1992).
Johnson-Flanagan AM, Singh J: Alteration of gene expression during the induction of freezing tolerance of Brassica napus suspension cultures. Plant Physiol 85: 699–705 (1987).
Josè-Estanyol M, Ruiz-Avila L, Puigdomènech P: A maize embryo-specific gene encodes a proline-rich and hydrophobic protein. Plant Cell 4: 413–423 (1992).
Krul WR: Enhancement and repression of somatic embryogenesis in cell cultures of carrot by cold pretreatment of stock plants. Plant Cell Tiss Organ Cult 32: 271–276 (1993).
Kyte J, Doolittle RF: A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105–132 (1982).
Laberge S, Castonguay Y, Vézina L-P: New cold- and drought-regulated gene from Medicago sativa. Plant Physiol 101: 1411–1412 (1993).
Lee SP, Chen THH: Molecular biology of plant cold hardiness development. In: PH Li, L Christersson (eds) Advances in Plant Cold Hardiness, pp. 1–29. CRC Press, Roca Baton, FL (1993).
Luo M, Lin L, Hill RD, Mohapatra SS: Primary structure of environmental stress and abscisic acid-inducible alfalfa protein. Plant Mol Biol 17: 1267–1269 (1991).
Luo M, Liu J-H, Mohapatra S, Hill RD, Mohapatra SS: Characterization of a gene family encoding abscisic acid- and environmental stress-inducible proteins of alfalfa. J Biol Chem 267: 15367–15374 (1992).
Mohapatra SS, Poole RJ, Dhindsa RS: Changes in protein patterns and translatable messenger RNA populations during cold-acclimation of alfalfa. Plant Physiol 84: 1172–1176 (1987).
Mohapatra SS, Poole RJ, Dhindsa RS: Alterations in membrane protein-profile during cold treatment of alfalfa. Plant Physiol 86: 1005–1007 (1988).
Mohapatra SS, Wolfraim L, Poole RJ, Dhindsa RS: Molecular cloning and relationship to freezing tolerance of cold-acclimation-specific genes of alfalfa. Plant Physiol 89: 375–380 (1989).
Monroy AF, Castonguay Y, Laberge S, Sarhan F, Vézina L-P, Dhindsa RS: A new cold-induced alfalfa gene is associated with enhanced hardening at subzero temperature. Plant Physiol 102: 873–879 (1993).
Nowak J, Matheson SL, McLean NL, Harvard P: Regenerative trait and cold hardiness in highly productive cultivars of alfalfa and red clover. Euphytica 59: 189–196 (1992).
Salts Y, Wachs R, Gruissen W, Barg R: Sequence coding for a novel proline-rich protein preferentially expressed in young tomato fruit. Plant Mol Biol 17: 149–150 (1991).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).
Sanger F, Nicklen S, Coulsen AR: DNA sequencing with chain termination inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Showalter AM: Structure and function of plant cell wall proteins. Plant Cell 5: 9–23 (1993).
Wolfraim LA, Langis R, Tyson H, Dhindsa RS: cDNA sequence, expression, and transcript stability of a cold acclimation-specific gene, cas 18, of alfalfa (Medicago falcata) cells. Plant Physiol 101: 1275–1282 (1993).
Yoshida S, Uemura M: Protein and lipid compositions of isolated plasma membranes from orchard grass (Dactylis glomerata L.) and changes during cold acclimation. Plant Physiol 75: 31–37 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Castonguay, Y., Laberge, S., Nadeau, P. et al. A cold-induced gene from Medicago sativa encodes a bimodular protein similar to developmentally regulated proteins. Plant Mol Biol 24, 799–804 (1994). https://doi.org/10.1007/BF00029861
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00029861