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Establishing micropropagation conditions for five Cotoneaster genotypes

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Abstract

Micropropagation from shoot tips of five Cotoneaster genotypes C. franchetii, C dammeri var. Radicans, C. dammeri ‘Skogholm’, C. dammeri ‘Eichholz N° 1′ and C. dammeri ‘Streib's Findling’) is described. For culture initiation and multiplication, a medium based on Murashige and Skoog basal salts was suitable for all genotypes. For multiplication, 4.4 μM 6-benzyladenine provided the optimum results, while auxins proved to be essential for rooting in vitro. However, direct acclimatization of unrooted microcuttings was more efficient than in vitro rooting and acclimatization of rooted plants.

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Abbreviations

BA:

6-benzyladenine

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MS:

Murashige & Skoog (1962) basal medium

NAA:

α-naphthaleneacetic acid

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Authors and Affiliations

  1. Station d'Amélioration des Espèces Fruitières et Ornementales, BP 57, 49071, Beaucouzé Cedex, France

    Carine Monier & Sergio J. Ochatt

Authors
  1. Carine Monier
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  2. Sergio J. Ochatt
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Monier, C., Ochatt, S.J. Establishing micropropagation conditions for five Cotoneaster genotypes. Plant Cell Tiss Organ Cult 42, 275–281 (1995). https://doi.org/10.1007/BF00029999

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  • DOI: https://doi.org/10.1007/BF00029999

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