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Identification of an immediate-early salicylic acid-inducible tobacco gene and characterization of induction by other compounds

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Abstract

Tobacco genes that are induced in response to salicylic acid (SA) treatment with immediate-early kinetics were identified by differential mRNA display. Detailed analysis of IS10a, one cDNA clone identified by this method, revealed induction within 30 min of treatment, with a peak of expression at 3 h, that decayed rapidly thereafter. Treatment with the protein synthesis inhibitor, cycloheximide (CHX), also caused induction of IS10a mRNA to comparable levels, but the IS10a mRNA continued to accumulate after 3 h of induction. In combination, CHX and SA led to a superinduction of IS10a mRNA levels that was also sustained. Half-maximal induction was evident at ca. 100–150 μM SA. In addition to SA, induction of IS10a occurred to varying degrees upon treatment with acetylsalicylic acid, benzoic acid, 2,4-dichlorophenoxyacetic acid, methyl jasmonate, and hydrogen peroxide, whereas treatment with other compounds had no effect. The proteins encoded by IS10a and a second highly homologous cDNA show sequence similarity to UDP-glucose: flavonoid glucosyltransferases.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

4HBA:

4-hydroxybenzoic acid

aa:

amino acid

bp:

base pair

nt:

nucleotide

ASA:

acetyl salicylic acid

BA:

benzoic acid

CHX:

Cycloheximide

MJ:

methyl jasmonate

PCR:

polymerase chain reaction

SA:

salicylic acid

TMV:

tobacco mosaic virus

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  1. Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, 10021, New York, NY, USA

    Diana M. Horvath & Nam-Hai Chua

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  1. Diana M. Horvath
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Horvath, D.M., Chua, NH. Identification of an immediate-early salicylic acid-inducible tobacco gene and characterization of induction by other compounds. Plant Mol Biol 31, 1061–1072 (1996). https://doi.org/10.1007/BF00040724

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