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Effect of Si-status on diel variation of intracellular free amino acids in Thalassiosira weissflogii under low-light intensity

  • Part Three: Cell Cycle and Productivity
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Abstract

Twenty-one intracellular free amino acids were analysed during a 12-12 h light-dark cycle, on duplicate axenic cultures of Thalassiosira weissflogii (clone Actin, Provasoli-Guillard CCMP) under either Si-sufficient or Si-starved conditions. Total concentrations ranged between 40 and 165 fmol/cell. Total level as well as individual levels of amino acids decreased during the dark period, and GLN/GLU ratio was lower during the dark period. All these results were correlated with the light-dark carbon metabolism of the algae and related to the protein synthesis at night. The Si-starved cultures showed a lower total level of FAA compare to the Si-sufficient cultures, especially in the light period. Silica status of the cells affected more the metabolites of the dark respiration than the photorespiratory metabolites SER and GLY. Si deprivation induced higher range of ALA and VAL, and a decrease of the TCA metabolites GLU & ASP. Additionally, the relative percentage of ASP increased under Si starvation, at the expense of GLU, and this shift was emphasized in the dark period.

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  1. CNRS, CEOBM, Station Biologique, place Teissier, BP 74, 29682, Roscoff Cédex, France

    Véronique Martin-Jézéquel

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Martin-Jézéquel, V. Effect of Si-status on diel variation of intracellular free amino acids in Thalassiosira weissflogii under low-light intensity. Hydrobiologia 238, 159–167 (1992). https://doi.org/10.1007/BF00048784

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