Abstract
The multiple insemination of Drosophila subobscura females in nature was studied by the analysis of single female offspring using two highly polymorphic enzyme systems. On qualitative grounds it was found that 23% of these females were inseminated at least twice. The ‘power of detection’ of one enzyme system was estimated using the already known multi-inseminated females detected by the other system. This permitted the true frequency estimation of multi-inseminated females, in nature, which is 0.276 (for 95% confidence limits it ranges from 0.186 to 0.417). In a laboratory experiment it was found that sperm from the second inseminating male displaces the sperm from the first male.
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Loukas, M., Vergini, V. & Krimbas, C.B. The genetics of Drosophila subobscura populations XVIII. Multiple insemination and sperm displacement in Drosophila subobscura. Genetica 57, 29–37 (1981). https://doi.org/10.1007/BF00057540
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DOI: https://doi.org/10.1007/BF00057540