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Conversion of 12-hydroxyoctadecanoic acid to 12,15-, 12,16- and 12,17-dihydroxyoctadecanoic acids with Bacillus sp. U88

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Summary

A new microbial isolate, Bacillus sp. U88, transformed 12-hydroxyoctadecanoic acid to 12,15-, 12,16- and 12,17-dihydroxyoctadecanoic acids when grown aerobically in 1 % yeast extract medium at 30°C and shaken at 250 rpm for 5 days. These three dihydroxyfatty acids were derivatized with Trimethylsilyl ether and identified by GC-MS analysis. The yield of 12,15-, 12,16- and 12,17-dihydroxyoctadecanoic acids after 5 day incubation were 2.8 %, 7.1 % and 1.1 %, respectively.

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References

  • Durst, F., Benveniste, I., Salaun, J-P. and Werck-Reichhart, D. (1992). Biochem. Soc. Transactions. 20:353–355.

    Google Scholar 

  • Gibson, T. and Gordon, R.E. (1974). In: Bergey's Mannual of Determinative Bacteriology, 8th eds. R.E.Buchanan and N.E. Gibbons, eds. PP. 529–550, Baltimore: Williams and Wilkins Co.

    Google Scholar 

  • Huang, J.-K., Keudell, K.C., Zhao, J., Klopfenstein, W.E., Wen, L., Bagby, M.O., Lanser, A.C., Plattner, R.D., Peterson, R.E., Abbott, T.P. and Weisleder, D. (1995). J, Am. Oil Chem. Soc. 72,323–326.

    Google Scholar 

  • Kleiman, R. and Spencer, G.F. (1973). J. Am. Oil Chem. Soc. 50,31–38.

    Google Scholar 

  • Knothe, G., Bagby, M.O., Peterson, R.E. and Hou, C.T. (1992). J. Am. Oil Chem. Soc. 69,367–371.

    Google Scholar 

  • Kyaw, H. “Numerical taxonomy of bacterial isolates from fish and freshwater,” M.S. Thesis (1991), Western Illinois University, Macomb, IL.

    Google Scholar 

  • Lanser, A.C., Plattner, R.D. and Bagby, M.O. (1992). J. Am. Oil Chem. Soc. 69, 363–366.

    Google Scholar 

  • Miura, Y and Fulco, A.J. (1974). J. Biol. Chem. 249,1881–1888.

    Google Scholar 

  • Soda, K. (1987). J. Am. Oil Chem. Soc. 64, 1254A.

  • Soda, K. and Kido, T. (1989). Jpn. Kokai Tokkyo Koho JP 01 51: 092.

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Names are necessary to report factually on available data; however the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of other that may also be suitable.

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Huang, J.K., Keudell, K.C., Seong, SJ. et al. Conversion of 12-hydroxyoctadecanoic acid to 12,15-, 12,16- and 12,17-dihydroxyoctadecanoic acids with Bacillus sp. U88. Biotechnol Lett 18, 193–198 (1996). https://doi.org/10.1007/BF00128678

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