Summary
The production of 5,8,11,14,17-cis-eicosapentaenoic acid (EPA) by Mortierella fungi was hgghly stimulated when they were grown in a medium containing linseed oil at low temperature. This phenomenon was suggested to be mainly due to low temperature-dependent production of EPA from arachidonic acid formed through the n-6 route, although conversion of α-linolenic acid in the added linseed oil to EPA through the n-3 route took place at the same time. The amount of EPA accumulated reached 1.88 mg/ml of culture broth (66.6 mg/g dry mycelia) on cultivation of M. alpina 1S-4 with linseed oil at 12° C.
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Dosanjhi BS, Higgs DA, Plotnikoff MD, Markert JR, Buckley JT (1988) Preliminary evaluation of canola oil, pork lard and marine lipid singly and in combination as supplemental dietary lipid source for juvenile fall chinook salmon. Aquaculture 68:325–343
Dyerberg J (1986) Linolenate-derived polyunsaturated fatty acids and prevention of atherosclerosis. Nutr Rev 44:125–134
Shimizu S, Shinmen Y, Kawashima H, Akimoto K, Yamaa H (1988a) Fungal mycelia as a novel source of eicosapentaenoic acid: activation of enzyme(s) involved in eicosapentaenoic acid production at low temperature. Biochem Biophys Res Commun 150:335–341
Shimizu S, Kawashima H, Shinmen Y, Akimoto K, Yamada H (1988b) Production of eicosapentaenoic acid by Mortierella fungi. J Am Oil Chem Soc 65:1455–1459
Shimizu S, Kawashima H, Akimoto K, Shinmen Y, Yamada H (1988c) Conversion of an oil containing α-linolenic acid to an oil containing eicosapentaenoic acid by Mortierella alpina 1S-4 at low temperature. In: The Society of Fermentation Technology Japan (ed) Abstracts of Annual Meeting of the Society of Fermentation Technology, Japan, Osaka, November 9–11, p 149
Shimizu S, Kawashima H, Shinmen Y, Akimoto K, Yamada H (1989a) Microbial conversion of an oil containing α-linolenic acid to an oil containing eicosapentaenoic acid. J Am Oil Chem Soc 66:342–347
Shimizu S, Akimoto K, Kawashima H, Shinmen Y, Yamada H (1989b) Production of dihomo-γ-linolenic acid by Mortierella alpina 1S-4. J Am Oil Chem Soc 66:237–241
Shinmen Y, Shimizu S, Akimoto K, Kawashima H, Yamada H (1989) Production of arachidonic acid by Mortierella fungi: selection of a potent producer and optimization of culture conditions for large-scale production. Appl Microbiol Biotechnol 31:11–16
Watanabe T, Oowa F, Kitajima C, Fujita S (1980) Relationship between dietary value of brine shrimp Artemia salina and their content of ω3 highly unsaturated fatty acids. Bull Jpn Soc Sci Fish 46:35–41
Yamada H, Shimizu S, Shinmen Y (1987) Production of arachidonic acid by Mortierella elongata 1S-5. Agric Biol Chem 51:785–790
Yamada H, Shimizu S, Shinmen Y, Kawashima H, Akimoto K (1988) Production of arachidonic acid and eicosapentaenoic acid by microorganisms. In: Applewite TH (ed) Proceedings of the world conference on biotechnology for the fats and oils industry, Hamburg, 1987, American Oil Chemists' Society, Champaign, III, USA. pp 173–177
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On leave from Suntory Ltd., Osaka 618, Japan
Offprint requests to: Sakayu Shimizu
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Shimizu, S., Kawashima, H., Akimoto, K. et al. Conversion of linseed oil to an eicosapentaenoic acid-containing oil by Mortierella alpina 1S-4 at low temperature. Appl Microbiol Biotechnol 32, 1–4 (1989). https://doi.org/10.1007/BF00164813
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DOI: https://doi.org/10.1007/BF00164813