Abstract
Degradation of alginate and its constituents, polymannuronate (polyM) and polyguluronate (polyG), by gut bacteria isolated from sea urchins and abalones in the northern part of Japan, were investigated. Bacterial counts in the guts of sea urchin S. intermedius, were 105 to 108 CFU/g, and in abalone H. discus hannai, counts ranged from 106 to 109 CFU/g. More than 80% of total 600 isolates were found to have alginolytic activity. The alginolytic bacteria were predominantly fermentative, but some differences were observed in their substrate specificity as well as between the flora in the gut of sea urchins and the abalones. Seventy percent of the alginolytic bacteria from the sea urchins showed no degrading preference for polyM or polyG blocks, and were able to degrade both the substrates simultaneously. Most of the alginolytic bacteria (96.6%) from sea urchins belonged to the genus Vibrio. The majority of alginolytic bacteria (68.0% on average) from abalones only degraded polyG and they were predominantly non-motile fermenters. From these results, it appeared that a different type of association exists between alginolytic gut microflora and the marine algal feeders with respect to the level of contribution by bacteria to the host's digestion of alginate.
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References
Baumann P, Schubert RHW (1984) Vbrionaceae, In: Krieg NR, Holt JG (eds) Bergey's manual of systematic bacteriology, vol. 1. Williams & Wilkins, Baltimore, pp 516–550.
Boyen C, Kloareg B, Polne-Fuller M, Gibor A (1990) Preparation of alginate lyase from marine molluscs for protoplast isolation in brown algae. Phycologia 29:173–181.
Breznak JA (1982) Intestinal microbiota of termites and other xylophagous insects. Annu Rev Microbiol 36:323–343
Eppley RW Lasker R (1959) Alginase in the sea urchin Strongylocentrotus purpuratus. Science 129:214–215.
Felbeck H, Childress JJ, Somero GN (1983) Biochemical interactions between molluscs and their algal and bacterial symbionts. In: Wilier KM, Hochachka PW (eds) Mollusca, Vol. 2. Academic Press, New York, pp 331–358.
Gacesa P, Wusteman FS (1990) Plate assay for simultaneous detection of alginate lyase and determination of substrate specificity. Appl Environ Microbiol 56:2265–2267
Grasdalen H, Larsen B, Smidsrød O (1979) A P.M.R. study of the composition and sequence of uronate residues in alginates. Carbohyd Res 68:23–31
Harris JM (1993) The presence, nature, and role of gut microflora in aquatic invertebrates: a synthesis. Microb Ecol 25:195–231
Harris JM, Seiderer LJ, Lucas MI (1991) Gut microflora of two saltmarsh detritivore Thalassinid prawns, Upogebia africana and Callianassa Kraussi. Microb Ecol 21:277–296
Haug A, Larsen B, Smidsrød O (1967) Studies on the sequence of uronic acid residues in alginic acid. Acta Chem Scand 21:691–704
Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey's manual of determinative bacteriology, 9th ed. Williams & Wilkins, Baltimore
Hungate RE (1966) The rumen and its microbes. Academic Press, New York
Kimura T (1961) A method for rapid detection of alginic acid digesting bacteria (in Japanese). Bull Fac Fish Hokkaido Univ 12:41–48
Kloareg B, Quatrano RS (1988) Structure of the cell walls of marine algae and ecophysiological functions of the matrix polysaccharides. Oceanogr Mar Biol Ann Rev 26:259–315
Kovacs N (1956) Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 29:703
Leifson E (1963) Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
Nakada HI, Sweeny PC (1967) Alginic acid degradation by eliminases from abalone hepatopancreas. J Biol Chem 242:845–851
Ohnishi T, Suzuki M, Kikuchi R (1985) The distribution of polysaccharide hydrolase activity in gastropods and bivalves. Bull Jpn Soc Sci Fish 51:301–308
Penman A, Sanderson GR (1972) A method for the determination of uronic acid sequence in alginates. Carbohyd Res 25:273–282
Prim P, Lawrence JM (1975) Utilization of marine plants and their constituents by bacteria isolated from the gut of echinoids (Echinodermata). Mar Biol 33:167–173
Stewart CS, Bryant MP (1988) The rumen bacteria. In: Hobson PN (ed) The rumen microbial ecosystem. Elsevier Applied Science, London, pp 21–75
Suzuki M, Kikuchi R, Ohnishi T (1984) The polysaccharide degradation activity in digestive tract of sea urchin Strongylocentrotus nudus. Bull Jpn Soc Sci Fish 50:1255–1260
Tsujino I, Saito T (1962) Studies on alginase. Part II. A new unsaturated uronide isolated from alginase hydrolysate. Agr Biol Chem 26:115–118
Uki N, Watanabe T (1992) Review of the nutritional requirement of abalone (Haliotis spp.) and development of more efficient artificial diets. In Shepherd SA, Tegner MJ, Guzmán del Próo SA (eds) Abalone of the world. Fishing News Books, Cambridge, England, pp 504–517
Unkles SE (1977) Bacterial flora of the sea urchin Echinus esculentus. Appl Environ Microbiol 34:347–350
Yano Y, Machiguchi Y, Sakai Y (1993) Digestive ability of Strongylocentrotus intermedius. Bull Jpn Soc Sci Fish 59:733
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Sawabe, T., Oda, Y., Shiomi, Y. et al. Alginate degradation by bacteria isolated from the gut of sea urchins and abalones. Microb Ecol 30, 193–202 (1995). https://doi.org/10.1007/BF00172574
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DOI: https://doi.org/10.1007/BF00172574