Skip to main content
SpringerLink
Log in

Genomic structure of the human CD53 gene

  • Original Articles
  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

The genomic structure of the gene encoding human pan-leukocyte surface glycoprotein CD53 (a member of the “tetraspan family” of membrane proteins) was determined. The gene consists of eight exons encoding all sequences found in cDNA and is spread over more than 26 kilobases of genomic DNA. The exon-intron organization of the CD53 gene is strikingly similar to the CD63 and TAPA-1 genes, which suggests a close evolutionary relationship between these genes. The 5′ end of the gene upstream of the first exon contains at least three close transcription start points (approximately 20 base pairs 5′ of the 5′ end of the published cDNA). The region upstream of the transcription initiation sites is not G+C rich; it contains potential binding sites for several transcriptional factors but no TATA or CCAAT boxes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Agura, E. D., Howard, M., and Collins, S. J. Identification and sequence analysis of the promoter for the leukocyte integrin β-subunit (CD18). A retinoic acid-inducible gene. Blood 79: 602–609, 1992

    Google Scholar 

  • Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. Basic local alignment search tool. J Mol Biol 215: 403–410, 1990

    Google Scholar 

  • Amiot, M. Identification and analysis of cDNA clones encoding CD53: a pan-leukocyte antigen related to membrane transport proteins. J Immunol 145: 4322–4325, 1990

    Google Scholar 

  • Andria, M. L., Hsieh, C.-L., Oren, R., Francke, U., and Levy, S. Genomic organization and chromosomal localization of the TAPA-1 gene. J Immunol 147: 1030–1036, 1991

    Google Scholar 

  • Angelisová, P., Vlček, C., Štefanová, I., Lipoldová, M., and Hořejší, V. The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics 32: 281–285, 1990

    Google Scholar 

  • Ausubel, F. M., Brent, R., Kingsten, R. E., Moore, D. D., Smith, J. A., Seidman, J. G., and Struhl, K. (eds.): Current Protocols in Molecular Biology, pp. 4.6.1–4.6.8, John Wiley, New York, 1987

    Google Scholar 

  • Azorsa, D. O., Hyman, J. A., and Hildreth, J. E. K. CD63/Pltgp 40 — a platelet activation antigen identical to the stage-specific, melanoma-associated antigen ME491. Blood 78: 280–284, 1991

    Google Scholar 

  • Bell, G. M., Seaman, W. E., Niemi, E. C., and Imboden, J. B. The OX-44 molecule couples to signaling pathways and is associated with CD2 on rat T lymphocytes and a natural killer cell line. J Exp Med 175: 527–536, 1992

    Google Scholar 

  • Bellacosa, A., Lazo, P. A., Bear, S. E., and Tsichlis, P. N. The rat leukocyte antigen MRC OX-44 is a member of a new family of cell surface proteins which appear to be involved in growth regulation. Mol Cell Biol 11: 2864–2872, 1991

    Google Scholar 

  • Boucheix, C., Benoit, P., Frachet, P., Billard, M., Worthington, R. E., Gagnon, J., and Uzan, G. Molecular cloning of the CD9 antigen. A new family of cell surface proteins. J Biol Chem 266: 117–122, 1991

    Google Scholar 

  • Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159, 1987

    Google Scholar 

  • Classon, B. J., Williams, A. F., Willis, A. C., Seed, B., and Stamenkovic, I. The primary structure of the human leukocyte antigen CD37, a species homologue of the rat MRC OX-44 antigen. J Exp Med 169: 1497–1502, 1989

    Google Scholar 

  • DiMartino, J. F. Novel sequence elements required for transcriptional regulation of CD45. J Cell Biochem (Suppl 16 A), p. 101, 1992

  • Ehrich, E., Craig, A., Poustka, A., Frischauf, A.-M., and Lehrach, H. A family of cosmid vectors with the multi-copy R6K replication origin. Gene 57: 229–237, 1987

    Google Scholar 

  • Fisher, R. C. and Thorley-Lawson, D. A. Characterization of the Epstein-Barr virus-inducible gene encoding the human leucocyte adhesion and activation antigen Blast-1 (CD48). Mol Cell Biol 11: 1614–1623, 1991

    Google Scholar 

  • Fors, L., Saavedra, R. A., and Hood, L. Cloning of the shark Po promoter using a genomic walking technique based on the polymerase chain reaction. Nucleic Acids Res 18: 2793–2799, 1990

    Google Scholar 

  • Gaugitsch, H. W., Hofer, E., Huber, N. E., Schnabl, E., and Baumruker, T. A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schisostoma mansoni antigen Sm23. Eur J Immunol 21: 377–384, 1991

    Google Scholar 

  • Gil, M. L., Vita, N., Lebel-Binay, S., Miloux, B., Chalon, P., Kaghad, M., Marchiol-Fournigault, C., Conjeaud, H., Caput, D., Ferrara, P., Fradelizi, D., and Quillet-Mary, A. A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. J Immunol 148: 2826–2833, 1992

    Google Scholar 

  • Hadam, M. R. Cluster report: CD53. In W. Knapp, B. Dörken, W. R. Gilks, E. P. Rieber, R. E. Schmidt, H. Stein, and A. E. G. Kr. Von dem Borne (eds.): Leucocyte Typing IV. White Cell Differentation Antigens, pp. 674–678, Oxford University Press, Oxford, 1989

    Google Scholar 

  • Hořejší, V. and Vlček, Č. Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. FEBS Lett 288: 1–4, 1991

    Google Scholar 

  • Hotta, H., Miyamoto, H., Hara, I., Takahashi, N., and Homma, M. Genomic structure of the ME491/CD63 antigen gene and functional analysis of the 5′-flanking regulatory sequences. Biochem Biophys Res Commun 185: 436–442, 1992

    Google Scholar 

  • Imai, T., Fukudome, K, Takagi, S., Nagira, M., Furuse, M., Fukuhara, N., Nishimura, M., Hinuma, Y, and Yoshie, O. C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced syncytium formation is a member of a new family of transmembrane proteins including CD9, CD37, CD53, and CD63. J Immunol 149: 2879–2886, 1992

    Google Scholar 

  • Kallin, B., de Martin, R., Etzold, T., Sorrentino, V., and Philipson, L. Cloning of a growth arrest-specific and transforming growth factor β-regulated gene, T11, from a epithelial cell line. Mol Cell Biol 10: 5338–5345, 1991

    Google Scholar 

  • Kitani, S., Berenstein, E., Mergenhagen, S., Tempst, P., and Siraganian, R. P. A cell surface glycoprotein of rat basophilic leukemia cells close to the high-affinity IgE receptor (FcɛRI). Similarity to human melanoma differentiation antigen ME491. J Biol Chem 266: 1903–1909, 1991

    Google Scholar 

  • Klemsz, M. J., McKercher, S. R., Celada, A., Van Beveren, Ch., and Maki, R. A. The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. Cell 61: 113–124, 1990

    Google Scholar 

  • Kozmík, Z., Wang, S., Dörfler, P., Adams, B., and Busslinger, M. The promoter of the CD19 gene is a target for the B-cell specific factor BSAP. Mol Cell Biol 12: 2662–2672, 1992

    Google Scholar 

  • Maniatis, T. E., Fritsch, F., and Sambrook, J. Molecular Cloning: A Laboratory Manual, pp. 7.79–7.83, Cold Spring Harbor Laboratory, Cold Spring Harbor, 1989

    Google Scholar 

  • Marken, J. S., Schieven, G. L., Hellström, I., Hellström, K. E., and Aruffo, A. Cloning and expression of the tumor-associated antigen L6 (clone/carcinoma). Proc Natl Acad Sci USA 89: 3503–3507, 1992

    Google Scholar 

  • Meeker, T. C., Loeb, J., Ayres, M., and Sellers, W. The human Pim-1 gene is selectively transcribed in different hematolymphoid cell lines in G+C rich housekeeping promoter. Mol Cell Biol 10: 1680–1688, 1990

    Google Scholar 

  • Metzelaar, M. J., Wijngaard, P. L. J., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., and Clevers, H. C. CD63 antigen — a novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. J Biol Chem 266: 3239–3245, 1991

    Google Scholar 

  • Mitchell, P. J. and Tjian, R. Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science 245: 371–378, 1989

    Google Scholar 

  • Miyake, M., Koyama, M., Seno, M., and Ikeyama, S. Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31–15, which inhibits cell motility. J Exp Med 174: 1347–1354, 1991

    Google Scholar 

  • Mueller, P. R. and Wold, B. In vivo footprinting of a muscle specific enhancer by ligation mediated PCR. Science 246: 780–786, 1989

    Google Scholar 

  • Oren, R., Takahashi, S., Doss, C., Levy, R., and Levy, S. TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Mol Cell Biol 10: 4007–4015, 1990

    Google Scholar 

  • Pahl, H. L., Rosmarin, A. G., and Tenen, D. G. Characterization of the myeloid-specific CD11b promoter. Blood 79: 865–870, 1992

    Google Scholar 

  • Postel, E. H., Mango, S. E., and Flint, S. J. A nuclease-hypersensitive element of the human c-myc promoter interacts with a transcription initiation factor. Mol Cell Biol 9: 5123–5133, 1989

    Google Scholar 

  • Prestidge, D. S. SIGNAL SCAN: A computer program that scans DNA sequences for eukaryotic transcriptional elements. CABIOS 7: 203–206, 1991

    Google Scholar 

  • Sanger, F., Nicklen, S., and Coulson, A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977

    Google Scholar 

  • Schanberg, L. E., Fleenor, D. E., Kutzberg, J., Haynes, B. F., and Kaufman, R. E. Isolation and characterization of the genomic human CD7 gene: structural similarity with the murine Thy-1 gene. Proc Natl Acad Sci USA 88: 603–607, 1991

    Google Scholar 

  • Schuler, G. D., Altschul, S. F., Lipman, D. J. A workbench for multiple alignment construction analysis. Proteins Struct Funct Genet 9: 180–190, 1991

    Google Scholar 

  • Shapiro, M. B. and Senapathy, P. RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. Nucleic Acids Res 15: 7155–7174, 1987

    Google Scholar 

  • Silver, J. and Keerikatte, V. Novel use of polymerase chain reaction to amplify cellular DNA adjacent to an integrated provirus. J Virol 63: 1924–1928, 1989

    Google Scholar 

  • Slupsky, J. R., Seehafer, J. G., Tang, S.-C., Masellis-Smith, A., and Shaw, A. R. E. Evidence that monoclonal antibodies against CD9 antigen induce specific association between CD9 and the platelet glycoprotein IIb-IIIa complex. J Biol Chem 264: 12289–12293, 1989

    Google Scholar 

  • Smale, S. T. and Baltimore, D. The “initiator” as a transcription control element. Cell 57: 103–113, 1989

    Google Scholar 

  • Szala, S., Kasai, Y., Steplewski, Z., Rodeck, U., Koprowski, H., and Linnenbach, A. J. Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens. Proc Natl Acad Sci USA 87: 6833–6837, 1990

    Google Scholar 

  • Taguchi, T., Bellacosa, A., Zhou, J., Lazo, P. A., Tsichlis, P. N., and Testa, J. R. Mapping of the OX-44 human leukocyte antigen gene to 1p12-p13 by flourescence in situ hybridization. Cytogenet Cell Genet 58: 1863, 1991

    Google Scholar 

  • Takahashi, S., Doss, C., Levy, S., and Levy, R. TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen. J Immunol 145: 2207–2213, 1990

    Google Scholar 

  • Vaickus, L. and Levy R. Antiproliferative monoclonal antibodies: detection and initial characterization. J Immunol 135: 1987–1997, 1985

    Google Scholar 

  • Virtaneva, K., Angelisová, P., Baumruker, T., Hořejší, V., Nevanlinna, H., and Schröder, J. The genes for CD37, CD53, and R2, all members of a novel gene family, are located on different chromosomes. Immunogenetics 37: 461–465, 1993

    Google Scholar 

  • Wright, M. D., Henkle, K. J., and Mitchell, G. F. An immunogenic Mr 23 000 integral membrane protein of Schisostoma mansoni worms that closely resembles a human tumor-associated antigen. J Immunol 144: 3195–3200, 1990

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 14220 Praha 4, 1083, Vídeňská, Czech Republic

    Vladimír Kořínek & Václav Hořejší

Authors
  1. Vladimír Kořínek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Václav Hořejší
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers L11670-71.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kořínek, V., Hořejší, V. Genomic structure of the human CD53 gene. Immunogenetics 38, 272–279 (1993). https://doi.org/10.1007/BF00188803

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00188803

Keywords

Search

Navigation