Summary
The distribution of serotonin-immunoreactive cells in the lung of 4 species of reptiles was investigated. Serotonin-containing cells were found forming groups in the interconnecting septa in all 4 species studied, and also as solitary cells in Testudo graeca and Mauremys caspica. Serotonin-containing cells were also localized in the intramural ganglia of Pseudemys scripta elegans and Testudo graeca. The present study confirms that serotonin is widely distributed in the lung of vertebrates.
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Becker K, Kathleen G, Monaghan BS, Silva OL (1980) Immunocytochemical localization of calcitonin in Kulchitsky cells of human lung. Arch Pathol Lab Med 104:196–198
Cutz E, Chan W, Track NS (1981) Bombesin, calcitonin and leuenkephalin immunoreactivity in endocrine cells of human lung. Experientia 37:765–767
Cutz E, Goniakowska-Witalinska L, Chan W (1986) An immunohistochemical study of regulatory peptides in lungs of amphibians. Cell Tissue Res 244:277–233
Dayer AM, De Mey J, Will JA (1985) Localization of somatostatin-, bombesin-, and serotonin-like immunoreactivity in the lung of the fetal Rhesus monkey. Cell Tissue Res 239:621–625
Diaugustine RP, Sonstegard KS (1984) Neuroendocrine-like (small granule) epithelial cells of the lung. Environ Health Perspect 55:271–295
Ghatei MA, Sheppard MN, O'Shaughnessy DJ, Adrian TE, McGregor GP, Polak JM, Bloom SR (1982) Regulatory peptides in the mammalian respiratory tract. Endocrinology 111:1248–1254
Goniakowska-Witalinska L (1980) Endocrine-like cells in the lungs of the newt, Triturus alpestris Laur. Cell Tissue Res 210:521–524
Goniakowska-Witalinska L (1981) Neuroepithelial bodies in the lung of the tree frog Hyla arborea L. A scanning and transmision electron microscopic study. Cell Tissue Res 217:435–441
Gosney JR, Sissons MCJ (1985) Widespread distribution of bronchopulmonary endocrine cells immunoreactive for calcitonin in the lung of the normal adult rat. Thorax 40:194–198
Grimelius L (1968) A silver nitrate stain for α2 cells in human pancreatic islets. Acta Soc Med Upsala 73:243–270
Lauweryns JM, Cokelaere M (1973) Hypoxia sensitive neuroepithelial bodies. Intrapulmonary secretory neuroreceptors, modulated by the CNS. Z Zellforsch 145:521–540
Lauweryns JM, Cokelaere M, Theunynck P (1973) Serotonin producing neuroepithelial bodies in rabbit respiratory mucosa. Science 180:410–413
Lauweryns JM, Bock V, Verhofstad AAJ, Steinbusch HWM (1982) Immunohistochemical localization of serotonin in intrapulmonary neuro-epithelial bodies. Cell Tissue Res 226:215–223
Lauweryns JM, Ranst LV, Verhofstad AAJ (1986) Ultrastructural localization of serotonin in the intrapulmonary neuroepithelial bodies of neonatal rabbits by use of immuno-electron microscopy. Cell Tissue Res 243:455–459
Linnoila RI, Becker KL, Silva OM, Snider RH, Moore CH F (1984) Calcitonin as a marker for diethylnitrosamine-induced pulmonary endocrine cell hyperplasia in hamsters. Lab Invest 51:39–45
Marchewsky AM, Kleinerman J (1982) Immunocytochemical studies of APUD cells in airways. Arch Pathol Lab Med 106:400–403
McLelland J, Mcfarlane CJ (1986) Solitary granular endocrine cells and neuroepithelial bodies in the lungs of the ringed turtle dove (Streptopelia risoria). J Anat 147:83–93
Pastor LM, Ballesta J, Hernández F, Pérez Tomás R, Zuasti A, Ferrer C (1987) A microscopic study of the tracheal epithelium of Testudo graeca and Pseudemys scripta elegans. J Anat (In press)
Polak JM, Bloom SR (1982) Regulatory peptides and neuron-specific enolase in the respiratory tract of man and other mammals. Exp Lung Res 3:313–328
Ravazzola M, Orci L, Girgis SI, Galan FG, McIntyre I (1981) The lung is the major organ source of calcitonin in the lizard. Cell Biol Int Rep 5:937–944
Rogers DC, Haller CJ (1978) Innervation and cytochemistry of the neuroepithelial bodies in the ciliated epithelium of the toad lung (Bufo marinus). Cell Tissue Res 195:395–410
Scheuermann dW, De Groodt-Lasseel MHA, Stilman C (1984) A light and fluorescence cytochemical and electron microscopic study of granule-containing cells in the intrapulmonary ganglia of Pseudemys scripta elegans. Am J Anat 171:377–394
Scheuermann DW, De Groodt-Lasseel MHA, Stilman C, Meisters ML (1983) A correlative light-, fluorescence and electron microscopic study of neuroepithelial bodies in the lung of the red-eared turtle Pseudemys scripta elegans. Cell Tissue Res 234:249–269
Sheppard MN, Marangos PJ, Bloom SR, Polak JM (1984) Neuron specific enolase: A marker for the early development of nerves and endocrine cells in the human lung. Life Sci 34:265–271
Sorokin SP, Hoyt RF, Grant MM (1983) Comparative biology of small granule cells and neuroepithelial bodies in the respiratory system: short review. Am Rev Respir Dis [Suppl] 128:s26-s31
Stahlman MJT, Kasselberg AG, Orth DN, Gray ME (1985) Ontogeny of neuroendocrine cells in human fetal lung. II An immunohistochemical study. Lab Invest 52:52–60
Sternberger LA (1979) The unlabeled antibody peroxidase-antiperoxidase (PAP) method. In: Sternberger (ed) Immunocytochemistry. John Wiley & Sons, New York, pp 104–169
Tsutsumi Y, Osamura Y, Watanabe K, Yasnaihara N (1983) Immunohistochemical studies on gastrin-releasing peptide and adrenocorticotropic hormone-containing cells in the human lung. Lab Invest 48:623–631
Verhofstad AAJ, Steinbusch HWM, Penke B, Varga J, Joosten HWJ (1981) Serotonin-immunoreactive cells in the superior cervical ganglion of the rat. Evidence for the existence of separate serotonin- and catecholamine-containing small ganglionic cells. Brain Res 156:75–82
Wasano K, Yamamoto T (1978) Monoamine-containing granulated cells in the frog lung. Cell Tissue Res 193:201–209
Wasano K, Yamamoto T (1979) APUD-type receptor-secretory cells in the chicken lung. Cell Tissue Res 201:197–205
Wharton J, Polak JM, Bloom SR, Ghatei MA, Solcia E, Brown ME, Pearse AGE (1978) Bombesin-like immunoreactivity in the lung. Nature 273:769–770
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Pastor, L.M., Ballesta, J., Perez-Tomas, R. et al. Immunocytochemical localization of serotonin in the reptilian lung. Cell Tissue Res. 248, 713–715 (1987). https://doi.org/10.1007/BF00216504
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DOI: https://doi.org/10.1007/BF00216504