Summary
In the American cockroach, the distribution and connections of neuronal elements of the terminal ganglion-proctodeal nerve-hindgut system were investigated by means of immunohistochemical methods and axonal CoCl2 iontophoresis. Proctolinlike immunoreactivity was localized within neurons of the terminal ganglion projecting into the proctodeal nerve on the one hand, and in nerve cells without a direct connection to this system on the other. Immunohistochemically, in whole mount preparations fibres of the proctodeal nerve and terminal structures in the hindgut musculature exhibit strong proctolinlike immunoreactivity. At the light- and electron-microscopic levels the pathways of about 30 somata of the proctodeal neural system were characterized by cobalt chloride iontophoresis. The relationships of cobalt filled and immunoreactive neuronal structures are discussed.
For the preparation of tritiated proctolin we thank Dr. S. Reißmann, WB Biochemie, Sektion Biologie, FSU Jena
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Reference
Agricola H, Eckert M, Ude J (in press) Course and connections of the proctolin-bearing proctodeal nerve in 6th abdominal ganglion of the cockroach, as revealed by electron microscopic, iontophoretic and immunohistochemical methods. In: IBRO monograph series
Brown BE (1967) Neuromuscular transmitter substance in insect visceral muscle. Science, Wash 155:595–597
Brown BE (1975) Proctolin: A peptide transmitter candidate in insects. Life Sci 17:1241–1252
Brown BE (1977) Occurrence of proctolin in six orders of insects. J Insect Physiol 23:861–864
Brown BE, Nagai T (1969) Insect visceral muscle: Neural relations of the proctodeal muscle of the cockroach. J Insect Physiol 15:1767–1783
Brown BE, Starrat AN (1975) Isolation of proctolin, a myotropic peptide, from Periplaneta americana. J Insect Physiol 21:1879–1881
Costa M, Buffa R, Furness JB, Solcia E (1980) Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparations. Histochemistry 65:157–165
Dubois M-P (1976) Mise en évidence par immunofluorescence d'un décapeptide, le LH-RF, dans l'éminence médiane. Ann Histochim 21:269–278
Farley RD, Evans SJ (1972) Neurosecretion in the terminal ganglion of the cockroach, Periplaneta americana. J Insect Physiol 18:289–303
Folkersen J, Teisner B, Ahrons S, Svehag S-E (1978) Affinity chromatographic purification of the pregnancy zone protein. J Immunol Meth 23:117–125
Holman GM, Cook BJ (1979) Evidence for proctolin and a second myotropic peptide in the cockroach, Leucophaea maderae, determined by bioassay and HPLC analysis. Insect Biochem 9:149–154
Mason CA (1973) New features of the brain-retrocerebral neuroendocrine complex of the locust Schistocerca vaga (Scudder). Z Zellforsch 141:19–32
Mason CA, Nishioka RS (1974) The use of the cobalt chloride-ammonium sulfide precipitation technique for the delineation of invertebrate and vertebrate neurosecretory systems. In: Knowles F, Vollrath L (eds) Neurosecretion — the final neuroendocrine pathway. Springer, Berlin Heidelberg New York, p 48–58
Rademakers LHPM (1977) Identification of a secretomotor centre in the brain of Locusta migratoria, controlling the secretory activity of the adipokinetic hormone producing cells in the corpus cardiacum. Cell Tissue Res 184:381–395
Starrat AN, Brown BE (1975) Structure of the pentapeptide proctolin, a proposed neurotransmitter in insects. Life Sci. 17:1253–1256
Sullivan RE (1979) A proctolin-like peptide in crab pericardial organs. J Exp Zool 210:543–552
Timm F (1958) Zur Histochemie der Schwermetalle. — Das Sulfid-Silber-Verfahren. Dtsch Z Gerichtl Med 46:701–711
Ude J, Agricola H (1979) Synaptic connections of the Nervus connectivus in the frontal ganglion of Periplaneta americana L. (Insecta). An electron microscopic and iontophoretic study. Cell Tissue Res 204:155–159
Walker RJ, James VA, Roberts CJ, Kerkut GA (1980) Neurotransmitter receptors in invertebrates. In: Satelle DB et al. (ed) Receptors for neurotransmitters, hormones and pheromones in insects. Elsevier/North-Holland Biomedical Press, Amsterdam, p 41–57
Author information
Authors and Affiliations
Additional information
The authors wish to thank G. Schörlitz, Film- und Bildstelle, FSU Jena, for photographs of whole mount preparations and Ms. A. Zinßer and Mrs. B. Cosack for excellent technical assistance.
Rights and permissions
About this article
Cite this article
Eckert, M., Agricola, H. & Penzlin, H. Immunocytochemical identification of proctolinlike immunoreactivity in the terminal ganglion and hindgut of the cockroach Periplaneta americana (L.). Cell Tissue Res. 217, 633–645 (1981). https://doi.org/10.1007/BF00219370
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00219370