Abstract
We describe here a two step procedure which allows the easy isolation of somatic embryos from Sunflower (Helianthus annuus L.) hypocotyl tissues. Thin cell layers composed of the epidermis plus 3 to 6 parenchyma cell layers were incubated for 5 days in a basal Murashige and Skoog medium using an auxin to cytokinin weight ratio of 1/1. The epidermis layers were then transferred to a Gamborg medium containing a high level of sucrose. After one week of incubation in this medium, many somatic embryos started to be released from the parental epidermal tissue. Even though the germination of these embryos is difficult, we have been able to induce secondary embryos and regenerate fertile plants.
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Abbreviations
- NAA:
-
1-naphthalene acetic acid
- IAA:
-
indole-3-acetic acid
- BAP:
-
6-benzylamino-purine
- MS:
-
Murashige and Skoog medium
- B5:
-
Gamborg medium
References
Ammirato P.V. (1983) In handbook of plant cell culture. Techniques for propagation and breeding, edited by Evans, Sharp, Ammirato,Yamada Macmillan editions, volume 1:82–123
Bigot C. (1976) Can. J. Bot. 54: 852–867
Chandler J.M. and Beard B.H. (1983) Crop Sci. 23: 1004–1007
Charest P.J., Holbrook L.A., Gabard J., Iyer V.N. and Miki B.L. (1988) Theor. Appl.Genet. 75: 438–445
Corbineau F., Rudnicki R.M. and Côme D. (1988) Plant Growth Regulation 7: 157–169
Dupuis J.M., Gomiero M., Plantevin C. and Chagvardieff P. (1988) C.R. Acad.Sci.Paris 307: 465–468
Finer J.J. (1987) Plant Cell Reports 6: 372–374
Freyssinet M. and Freyssinet G. (1988) Plant Science 56: 177–181
Gamborg O.L., Miller R.A. and Ojimak (1968) Exptl. Cell Research 50: 151–158
Greco B., Tanzarella O.A., Carrozzo G. and Blanco A. (1984) Plant Science Letters 36: 73–77
Klimaszewska K. and Keller W.A. Plant Cell Tissue Organ Culture (1985) 4: 183–197
Lupi M.C., Bennici A., Locci F. and Gennai D. (1987) Plant Cell, Tissue and Organ Culture 11: 47–55
Moyne A.L., Thor V., Pélissier B., Bergounioux C., Freyssinet G and Gadal P. (1988) Plant Cell Reports 7: 437–440
Murashige T. and Skoog F. (1962) Physiol. Pant. 15: 473–497
Paterson K.E. and Everett N.P. (1985) Plant Science 42: 125–132
Pépin R. and Boumendil J. (1982) Cytologia 47: 359–377
Power C.J. (1987) Amer. J. Bot. 74: 497–503
Spurr A.R. (1969) J. Ultrastruct. Res. 26: 31–43
Tran Thanh Van M., Dien N.T. and Chlyah A. (1974) Planta 119: 149–159
Trinh T.H., Mante S., Pua E-C. and Chua N-H. (1987) Bio/Technology 5: 1081–1084
Wilcox McCann A., Cooley G. and Van Dreser J (1988) Plant Cell, Tissue and Organ Culture 14: 103–110
Williams E.G. and Maheswaran G. (1986) Annals of Botany 57: 443–462
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Communicated by I.K. Vasil
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Pélissier, B., Bouchefra, O., Pépin, R. et al. Production of isolated somatic embryos from sunflower thin cell layers. Plant Cell Reports 9, 47–50 (1990). https://doi.org/10.1007/BF00232134
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DOI: https://doi.org/10.1007/BF00232134