Summary
The inactivation of λ repressor was followed by the specific DNA binding assay during the course of lysogenic induction provoked by incubation at 42°C of an E. coli tif-1 lysogenic strain. The presence of up to 400 μg/ml chloramphenicol during the inducing treatment did not impair the loss of repressor binding activity, whilst concentrations of 200 μg/ml neomycin and 100 μg/ml rifampicin effectively inhibited the inactivation of λ repressor.
Residual protein synthesis in the presence of chloramphenicol, neomycin and rifampicin was 5%, 5% and 27% respectively of that observed in the drug-free control. This residual synthesis did not appear to involve amplification of the X-protein. These results suggest that tif-mediated inactivation of the λ repressor requires the activation of some specific gene(s), the translation of which appears to be resistant to chloramphenicol.
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Bailone, A., Blanco, M., Devoret, R.: E. coli K12 inf: a mutant deficient in prophage λ induction and cell filamentation. Molec. gen. Genet. 136, 291 (1975)
Bollum, F. J.: Filter paper disk techniques for assaying radioactive macromolecules. In: Procedures in nucleic acid research (Cantoni, G. L. and Davies, D.R., eds.), Vol. I, pp. 296–300 New York: Harper and Row 1967
Castellazzi, M., George, J., Buttin, G.: Prophage induction and cell division in E. coli. I. Further characterization of a thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation. Molec. gen. Genet. 119, 139 (1972)
Castellazzi, M., Morand, Ph., George, J., Buttin, G.: Prophage induction and cell division in E. coli. V. Dominance and complementation analysis in partial diploids with pleiotropic mutations (tif, recA, zab and lexB) at the recA locus. Molec. gen. Genet. 153, 297 (1977)
Defais, M., Caillet-Fauquet, P., Fox, M., Radman, M.: Induction kinetics of mutagenic DNA repair activity in E. coli following ultraviolet irradiation. Molec. gen. Genet. 148, 125 (1976)
Defais, M., Fauquet, P., Radman, M., Errera, M.: Ultraviolet mutagenesis of λ in different genetic systems. Virology 43, 495 (1971)
Donch, J., Greenberg, J., Green, M.: Repression of induction by UV of λ phage by extrA mutations in E. coli. Genet. Res. Camb. 15, 87 (1970)
Emmerson, P. T., West, S. C.: Identification of protein X of E. coli as the recA +/tif+ gene product. Molec. gen. Genet. 155, 77, (1977)
Goldthwait, D., Jacob, F.: Sur le mécanisme de l'induction du développment du prophage chez les bactéries lysogènes. C.R. Acad. Sci. (Paris) 259, 661 (1964)
Gudas, L.J. Mount, D.W.: Identification of the recA (tif) gene product of E. coli. Proc. nat. Acad. Sci. (Wash.) 74, 5280 (1977)
Hayes, W.: Phenotypic instability in a tif-1 mutant of E. coli. II. Recessiveness of the tif-1 mutation. Molec. gen. Genet. 147, 67 (1976)
Hertman, I., Luria, S.E.: Transduction studies on the role of a rec + gene in the ultraviolet induction of prophage lambda. J. molec. Biol. 23, 117 (1967)
Kirby E.P., Jacob, F., Goldthwait, D.A.: Prophage induction and filament formation in a mutant strain of E. coli Proc. nat. Acad. Sci. (Wash.) 58, 1903 (1967)
Laemli, U.K., Favre, M.: Muturation of the head of bacteriophage T4. J. molec. Biol. 80, 575 (1973)
Lowry, O.H., Rosenbrough, N.J., Fan, A.I., Randall, R.J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265 (1951)
Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. molec. Biol. 3, 208 (1961)
McEntee, K.: Protein X is the product of the recA gene of E. coli. Proc. nat. Acad. Sci. (Wash.) 74, 5275 (1977)
Mindlin, S.Z., Ilyina, T.S., Vocykova, T.A., Velkov, U. V.: Genetic analysis of rifampicin resistant mutants of E. coli K12. Molec. gen. Genet. 115, 115 (1972)
Miura, A., Tomizawa, J.: Studies on radiation sensitive mutants of E. coli. III. Participation of the rec system in induction of mutation by ultraviolet irradiation. Molec gen. Genet. 103, 1 (1968)
Monk, M., Kinross, J.: The kinetics of derepression of prophage λ following ultraviolet irradiation of lysogenic cells. Molec. gen. Genet. 137, 263 (1975)
Pestka, S.: Inhibitors of protein synthesis. In: Molecular mechanism of protein biosynthesis (Weissbach, H., Pestka, S., eds.), p 467. New York: Academic Press 1977
Ptashne, M.: Specific binding of λ repressor to DNA. Nature (Lond.) 214, 232 (1967)
Radman, M.: Phenomenology of an inducible mutagenic DNA repair pathway in E. coli: SOS repair hypothesis. In: Molecular and environmental aspects of mutagenesis (Prakash, L., Sherman, F., Miller, M. W., Lawrence, C. W., Taber, H. W., eds.), p. 128. Springfield Illinois: C. C. Thomas Publishing Co 1974
Riva, S., Silvestri, L. G. Rifampicin: a general view. Ann. Rev. Microbiol. 26, 199 (1972)
Roberts, J. W., Roberts, C. W.: Proteolytic cleavage of bacteriophage lambda repressor in induction. Proc. nat. Acad. Sci. (Wash.) 72, 147 (1975)
Roberts, J. W., Roberts, C. W., Mount, D.: Inactivation of λ repressor in vitro. Proc. nat. Acad. Sci. (Wash._ 74, 2283 (1977)
Ruff, W., Kirby, P., Goldthwait, D. A.: Cell division and prophage induction in E. coli. Studies of nucleotide levels. J. Bact. 106, 994 (1971)
Schekman, R. W., Iwaya, M., Bromstrup, K., Denhardt, D. T.: The mechanism of replication of ΦX174 single-stranded DNA. III. An enzymic study of the structure of the replicative from II DNA. J. molec Biol 57, 177 (1971)
Shinagawa H., Itoh, T.: Inactivation of DNA binding activity of repressor in extracts of λ lysogens treated with mitomycin C. Molec. gen. Genet. 126, 103 (1973)
Shinagawa, H., Mizuuchi, K., Emmerson, P. T.: Induction of prophage lambda by λ-rays, mitomycin C and tif; repressor cleavage studied by immunoprecipitation. Molec. gen. Genet. 155, 87 (1977)
Susskind, M., Botstein, D.: Mechanism of action of P22 antirepressor. J. molec. Biol. 98, 413 (1975)
Tomizawa, J., Ogawa, T.: Effect of UV irradiation on bacteriophage λ immunity. J. molec. Biol. 23, 247 (1967)
West, St., Powell, K., Emmerson, P.: RecA + dependent inactivation of the λ repressor in E. coli lysogens by radiation and by tif expression. Molec. gen. Genet. 141, 1 (1975)
Witkin, E.: Thermal enhancement of ultraviolet mutability in a tif-1 uvrA derivative of E. coli B/r: evidence that ultraviolet mutagenesis depends upon an inducible function. Proc. nat. Acad. Sci. (Wash.) 71, 1930 (1974)
Witkin, E.: Persistence and decay of thermoinducible error-prone repair activity in nonfilamentous derivatives of tif-1 E. coli B/r: the timing of some critical events in ultraviolet mutagenesis. Molec. gen. Genet. 142, 87 (1975)
Witkin, E.: Ultraviolet mutagenesis and inducible DNA repair in E. coli. Bact. Rev. 40, 869 (1976)
Zillig, W., Zechel, K., Rabussay, D., Schachna, M., Sethi, V.S., Palm, M. P., Hal, A., Seifert, W.: On the role of different subunits of DNA-dependent RNA polymerase from E. coli in the transcription process. Cold Spr. Harb. Symp. quant. Biol. 35. 47 (1970)
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Communicated by B.A.Bridges
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Maenhaut-Michel, G., Brandenburger, A. & Boiteux, S. Requirement of protein and RNA synthesis for λ repressor inactivation by tif-1: Effects of chloramphenicol, neomycin and rifampicin. Molec. Gen. Genet. 163, 293–299 (1978). https://doi.org/10.1007/BF00271958
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DOI: https://doi.org/10.1007/BF00271958