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Increased accumulation of indole alkaloids by some cell lines of Catharanthus roseus in response to addition of vanadyl sulphate

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Abstract

Vanadyl sulphate (10–500 mg/l), when added to cell suspension cultures of Catharanthus roseus stimulated increased intracellular accumulation of catharanthine and ajmalicine. This response was demonstrated in both flask and fermenter (30 litre) systems. The response varied, and depended upon cell line, concentration of vanadyl sulphate and the stage of the growth phase at which the cells were treated. This process has the potential to increase the yield and reduce the production time for commercially useful secondary plant metabolites.

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Abbreviations

Ajm:

ajmalicine

Cath:

catharanthine

CAS:

ceric ammonium sulphate

VOSO4 :

vanadyl sulphate

FW:

fresh weight

n.d.:

not detected

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Authors and Affiliations

  1. Allelix Inc., 6850 Goreway Drive, L4V 1P1, Mississauga, Ontario, Canada

    J. I. Smith, N. J. Smart & M. Misawa

  2. Plant Biotechnology Institute, National Research Council of Canada, S7N OW9, Saskatoon, Saskatchewan, Canada

    W. G. W. Kurz

  3. Centre for Plant Biotechnology, Department of Botany, University of Toronto, M5S 1A1, Ontario, Canada

    S. G. Tallevi & F. DiCosmo

  4. Institute for Biomedical Engineering, University of Toronto, M5S 1A1, Toronto, Ontario, Canada

    F. DiCosmo

Authors
  1. J. I. Smith
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  2. N. J. Smart
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  3. M. Misawa
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  4. W. G. W. Kurz
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  5. S. G. Tallevi
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  6. F. DiCosmo
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Communicated by F. Constabel

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Smith, J.I., Smart, N.J., Misawa, M. et al. Increased accumulation of indole alkaloids by some cell lines of Catharanthus roseus in response to addition of vanadyl sulphate. Plant Cell Reports 6, 142–145 (1987). https://doi.org/10.1007/BF00276673

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  • DOI: https://doi.org/10.1007/BF00276673

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