Abstract
Lipopolysaccharides were isolated from the moderate halophilic Ectothiorhodospira shaposhnikovii slight to and Ectothiorhodospira mobilis and from the extremely halophilic Ectothiorhodospira halophila by the hot phenol-water and purified by the phenol-chloroform-petroleum ether methods. The isolated lipopolysaccharides of all three species contained 3-deoxy-d-manno-octulosonic acid and d-glycero-d-mannoheptose indicating the existence of a core. They contained additionally glucose and uronic acids (E. shaposhnikovii and E. mobilis) or glucose, uronic acids and threonine (E. halophila). Sodium deoxycholate gel-electrophoresis of the three lipopolysaccharides, each showing only one major band, indicated R-type character of the lipopolysaccharides of the three Ectothiorhodospira species.
The lipid A fractions of the lipopolysaccharides from E. shaposhnikovii and E. mobilis represented phosphorylated “mixed” lipid A types with both 2,3-diamino-2,3-dideoxy-d-glucose and d-glucosamine. The lipid A from E. halophila contained also phosphate and 2,3-diamino-2,3-dideoxy-d-glucose but only traces of d-glucosamine, which would indicated lipid ADAG. The fatty acid spectra were characterized by amide-bound 3-OH-10:0 and 3-OH-12:0 (E. shaposhnikovii), 3-OH-10:0 (E. mobilis), or 3-OH-10:0,3-OH-14:0, and 3-oxo-14-0 (E. halophila). The predominant ester-bound fatty acids were 14:0 and 16:0 (E. shaposhnikovii and E. mobilis), or 12:0 and 14:1 (E. halophila).
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Abbreviations
- DAG:
-
2,3-diamino-2,3-dideoxy-d-glucose
- Kdo:
-
3-deoxy-d-manno-octulosonic acid
- GlcA:
-
glucuronic acid
- GalA:
-
galacturonic acid
- GC-MS:
-
combined gas liquid chromatographymass spectrometry
- GlcN:
-
Glucosamine
- DOC:
-
sodium deoxycholate
- LPS:
-
lipopolysaccharide
- PAGE:
-
polyacrylamide gel electrophoresis
- PCP:
-
phenol-chloroform-petroleum ether
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Zahr, M., Fobel, B., Mayer, H. et al. Chemical composition of the lipopolysaccharides of Ectothiorhodospira shaposhnikovii, Ectothiorhodospira mobilis, and Ectothiorhodospira halophila . Arch. Microbiol. 157, 499–504 (1992). https://doi.org/10.1007/BF00276769
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DOI: https://doi.org/10.1007/BF00276769