Summary
The Sau3A family is a human, clustered, highly repetitive, GC-rich DNA family. In situ hybridization studies with a plasmid carrying a Sau3A monomer as a probe have shown that Sau3A sequences are preferentially concentrated in the heterochromatic regions of human acrocentric chromosomes (D and G groups, both in pericentromeric regions and in cytological satellites) and in pericentromeric heterochromatin of chromosome 1. The same chromosomal locations were observed by using as probes two recombinant phages which carry Sau3A-positive genomic sectors. The two sectors differfor the relative proportions of monomer and multiples of Sau3A repeats, which show different extents of homology to the cloned monomer, and for the presence, in one of the two, of a samll amount of an unrelated repeat (alphoid DNA). The similarity of the results obtained with the three probes suggests that heterogeneous Sau3A repeats share the same chromosomal localizations and that the two analyzed genomic sectors may not contain significant amounts of repetitive DNAs other than the Sau3A family. A comparison between the chromosomal locations of Sau3A and EcoRI families of repeats has confirmed that each family is characterized by specific chromosomal locations and that single heterochromatic regions may contain both.
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References
Beauchamp RS, Mitchell AR, Buckland RA, Bostock CJ (1979) Specific arrangements of human satellite III DNA sequences in human chromosomes. Chromosoma 71:153–166
Burk RD, Szabo P, O'Brien S, Nash WG, Yu L, Smith KD (1985) Organization and chromosomal specificity of autosomal homologs of human Y chromosome repeated DNA. Chromosoma 92:225–233
Cooke HJ (1976) Repeated sequence specific to human males. Nature 262:182–186
Cooke HJ, Hindley J (1979) Cloning of human satellite III DNA: different components are on different chromosomes. Nucleic Acids Res 6:3177–3197
Corneo G, Ginelli E, Polli E (1967) A satellite DNA isolated from human tissues. J Mol Biol 23:619–622
Corneo G, Ginelli E, Polli E (1970) Repeated sequences in human DNA. J Mol Biol 48:319–327
Corneo G, Ginelli E, Polli E (1971) Renaturation properties and location in heterochromatin of human satellite DNAs. Biochim Biophys Acta 247:528–534
Devine EA, Nolin SL, Houck GE, Jenkins EC, Brown TW (1985) Chromosomal location of several families of repetitive sequences by in situ hybridization. Am J Med Genet 37:114–123
Godsen IR, Lawrie SS, Godsen CM (1981) Satellite DNA sequences in the human acrocentric chromosomes: information from translocations and heteromorphisms. Am J Hum Genet 33:243–251
Harper ME, Saunders GF (1982) Localization of single copy sequences on G-banded chromosomes by in situ hybridization. Chromosoma 83:431–439
Jeanpierre M, Weil D, Gallano P, Creau-Goldberg N, Junien C (1985) The organization of two related subfamilies of a human tandemly repeated DNA is chromosome specific. Hum Genet 70: 302–310
Jones K, Corneo G (1971) Location of satellite and homogeneous DNA sequences on human chromosomes. Nature 233:268–271
Jones K, Prosser J, Corneo G, Ginelli E (1973) The chromosomal location of human satellite DNA III. Chromosoma 42:445–451
Jones K, Purdom IF, Prosser J, Corneo G (1974) The chromosomal location of human satellite DNA I. Chromosoma 49:161–171
Kunkel LM, Smith KD, Boyer SH (1976) Human Y-chromosome specific reiterated DNA. Science 191:1189–1190
Kunkel LM, Smith KD, Boyer SH (1979) Organization and heterogeneity of sequences within a repeating unit of human Y chromosome DNA. Biochemistry 28:3343–3353
Maniatis T, Fritsch EF, Sambrook J (eds) (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Manuelidis L (1978a) Complex and simple sequences in human repeated DNAs. Chromosoma 66:1–21
Manuelidis L (1978b) Chromosomal location of complex and simple repeated human DNAs. Chromosoma 66:23–32
Meneveri R, Agresti A, Ginelli E (1984) Distribution of repeated DNA families in the human genome. Biochem Biophys Res Commun 124:400–406
Meneveri R, Agresti A, Della Valle G, Talarico D, Siccardi AG, Ginelli E (1985) Identification of a human clustered GC-rich DNA family of repeats (Sau3A family). J Mol Biol 186:483–490
Mitchell AR, Beauchamp RS, Bostock CJ (1979) A study of sequence homologies in four satellite DNAs of man. J Mol Biol 135:127–149
Rackwitz HR, Zehetner G, Murialdo H, Delius H, Chai JH, Poustka A, Frischauf A, Lehrach H (1985) Analysis of cosmids using linearization by phage lamda terminase. Gene 40:259–266
Rigby PWJ, Dieckmann H, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251
Schmid M, Vogel W, Krone W (1975) Attraction between centric heterochromatin of human chromosomes. Cytogenet Cell Genet 15:66–80
Singer MF (1982) Highly repeated sequences in mammalian genomes. Int Rev Cytol 76:67–112
Southern EM (1975) Detection of specific DNA sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–527
Tuck-Muller CM, Bordson BL, Varela M, Bennett JW (1984) NOR associations with heterochromatin. Cytogenet Cell Genet 38:165–170
Waye JS, Willard HF (1985) Chromosome-specific alpha satellite DNA: nucleotide sequence analysis of the 2.0 kilobasepair repeat from the human X-chromosome. Nucleic Acids Res 13:2731–2743
Willard HF (1984) Chromosome-specific organization of human alphoid repeated DNA. Cytogenet Cell Genet 37:608–609
Willard HF, Smith KD, Sutherland J (1983) Isolation and characterization of a major tandem repeat family from the human X chromosome. Nucleic Acids Res 11:2017–2033
Wu JC, Manuelidis L (1980) Sequence definition and organization of a human repeated DNA. J Mol Biol 42:363–386
Yang TP, Hansen SK, Oishi KK, Ryder OA, Hamkalo BA (1982) Characterization of a cloned repetitive DNA sequence concentrated on the human X chromosome. Proc Natl Acad Sci USA 79: 6593–6597
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Agresti, A., Rainaldi, G., Lobbiani, A. et al. Chromosomal location by in situ hybridization of the human Sau3A family of DNA repeats. Hum Genet 75, 326–332 (1987). https://doi.org/10.1007/BF00284102
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DOI: https://doi.org/10.1007/BF00284102