Skip to main content
SpringerLink
Log in

The number of tartrate-resistant acid phosphatase-positive osteoclasts on neonatal mouse parietal bones is decreased when prostaglandin synthesis is inhibited and increased in response to prostaglandin E2, parathyroid hormone, and 1,25 dihydroxyvitamin D3

  • Laboratory Investigations
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Abstract

The culture of parietal bones from 4-day old mice in indomethacin (Ind) for 1 day caused a large reduction in the number of tartrate-resistant acid phosphatase positive osteoclasts (TRAP+OC) relative to both control bones and to freshly isolated bones. This reduction did not occur if prostaglandin E2 (PGE2) was present. When 5-bromo-2′-deoxyuridine (BDU) was injected into 4-day old mice, newly formed TRAP+OC nuclei became labeled 1 day later; these bones were then cultured with Ind for 1 day. TRAP+OC and newly labeled TRAP+OC nuclei were commensurately decreased in number. This suggests an active down-regulation rather than merely the inhibition of new TRAP+OC formation. Incubation of bones with Ind and either PGE2, parathyroid hormone, or 1,25 dihydroxyvitamin D3 for 6 hours following a 1-day preincubation in Ind, resulted in an increase in TRAP+OC compared with Ind alone. Using BDU labeling in vitro and in vivo, we show that this increase in number of TRAP+OC is not the result of cell proliferation, but rather differentiation of postmitotic precursors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Chambers TJ, Magnus CJ (1982) Calcitonin alters the behavior of isolated osteoclasts. J Pathol 136:27–33

    Google Scholar 

  2. Chambers TJ, Ali NN (1983) Inhibition of osteoclastic motility by prostaglandins I2E1,E2 and 6-oxoE1. J Pathol 139:383–397

    Google Scholar 

  3. Rodan GA, Martin TJ (1981) Role of osteoblasts in control of bone resorption—a hypothesis. Calcif Tissue Int 33:349–351

    Google Scholar 

  4. Chambers TJ, McSheehy PMJ, Thomson BM, Fuller K (1985) The effect of calcium-regulating hormones and prostaglandins on bone resorption by osteoclasts disaggregated from neonatal rabbit bones. Endocrinology 116:234–239

    Google Scholar 

  5. Thomson BM, Saklatvala J, Chambers TJ (1986) Osteoblasts mediate interleukin 1 stimulation of bone resorption by rat osteoclasts. J Exp Med 164:104–112

    Google Scholar 

  6. McSheehy PMJ, Chambers TJ (1986) Osteoblast-like cells in the presence of parathyroid hormone release soluble factor that stimulates osteoclastic bone resorption. Endocrinology 119: 1654–1659

    Google Scholar 

  7. Minkin C (1982) Bone acid phosphatase as a marker of osteoclast function. Calcif Tissue Int 34:285–290

    Google Scholar 

  8. Helfrich MH, Mieremet RHP, Thesingh CW (1989) Osteoclast formation in vitro from progenitor cells present in the adult mouse circulation. J Bone Miner Res 4:325–331

    Google Scholar 

  9. Hattersley G, Chambers TJ (1989) Generation of osteoclastic function in mouse bone marrow cultures: multinuclearity and tartrate-resistant acid phosphatase are unreliable markers for osteoclastic differentiation. Endocrinology 124:1689–1696

    Google Scholar 

  10. Modderman WE, Tuinenburg-Bol Raap AC, Nijweide PJ (1991) Tartrate-resistant acid phosphatase is not an exclusive marker for mouse osteoclasts in cell culture. Bone 12:81–87

    Google Scholar 

  11. Chambers TJ, Fuller K, Darby JA (1987) Hormonal regulation of acid phosphatase release by osteoclasts disaggregated from neonatal rat bone. J Cell Physiol 132:90–96

    Google Scholar 

  12. Zaidi M, Moonga B, Moss DW, MacIntyre I (1989) Inhibition of osteoclastic acid phosphatase abolishes bone resorption. Biochem Biophys Res Comm 159:68–71

    Google Scholar 

  13. Lerner UH (1987) Modification of the mouse calvarial technique improve responsiveness to stimulators of bone resorption. J Bone Miner Res 2:375–383

    Google Scholar 

  14. Shacter E, Lopez RL, Pati S (1991) The myeloperoxidase-H2O2-Cl system of neutrophils is inhibited by indomethacin and other non-steroidal anti-inflammatory drugs. Biochem Pharmacol 41:975–984

    Google Scholar 

  15. Klein DC, Raisz LG (1970) Prostaglandins: stimulation of bone resorption in tissue culture. Endocrinology 86:1436–1440

    Google Scholar 

  16. Thompson DD, Rodan GA (1988) Indomethacin inhibition of tenotomy-induced bone resorption in rats. J Bone Miner Res 3:409–414

    Google Scholar 

  17. Rifkin BR, Baker RL, Coleman SJ (1980) Effects of prostaglandin E2 on macrophages and osteoclasts in cultured fetal long bones. Cell Tissue Res 207:341–346

    Google Scholar 

  18. Akatsu T, Takahashi N, Debai K, Morita I, Murota S, Nagata N, Takatani O, Suda T (1989) Prostaglandins promote osteoclastlike cell formation by a mechanism involving cyclic adenosine 3′,5′-monophosphate in mouse bone marrow cell cultures. J Bone Miner Res 4:29–35

    Google Scholar 

  19. Collins DA, Chambers TJ (1991) Effect of prostaglandins E1, E2 and F2alpha on osteoclast formation in mouse bone marrow cultures. J Bone Miner Res 6:157–165

    Google Scholar 

  20. Collins DA, Chambers TJ (1992) Prostaglandin E2 promotes osteoclast formation in murine hematopoietic culture through an action on hematopoietic cells. J Bone Miner Res 7:555–561

    Google Scholar 

  21. Marshall MJ, Davie MWJ (1991) An immunocytochemical method for studying the kinetics of osteoclast nucleic on intact mouse parietal bone. Histochem J 23:402–408

    Google Scholar 

  22. Holt I, Davie MWJ, Braidman IP, Marshall MJ (1994) TRAP-positive osteoclasts in mouse parietal bones are acutely regulated by endogenous and exogenous prostaglandins. Bone (abstract) 15:238

    Google Scholar 

  23. Holt I, Davie MWJ, Braidman IP, Marshall MJ (1994) IL-6 is produced by PGE2-stimulated neonatal mouse parietal bone cultures but it does not cause the resulting osteoclast differentiation or bone resorption. Bone (abstract) 15:455

    Google Scholar 

  24. Holt I, Davie MWJ, Braidman IP, Marshall MJ (1994) Interleukin-6 does not mediate the stimulation by prostaglandin E2, parathyroid hormone or 1,25 dihydroxyvitamin D3 of osteoclast differentiation and bone resorption in neonatal mouse parietal bones. Calcif Tissue Int 55:114–119

    Google Scholar 

  25. Marshall MJ, Holt I, Davie MWJ (1993) Osteoclast recruitment in mice is stimulated by (3-amino-1-hydroxypropylidene)-1,1-bisphosphonate. Calcif Tissue Int 52:21–25

    Google Scholar 

  26. Lorenzo JA, Quinton J, Sousa S, Raisz LG (1986) Effects of DNA and prostaglandin synthesis inhibitors on the stimulation of bone resorption by epidermal growth factor in fetal rat long-bone cultures. J Clin Invest 77:1897–1902

    Google Scholar 

  27. Abe K, Ohno K, Hasegawa K (1990) Morphological relationships between osteoclasts and bone resorption surfaces on mouse parietal bones. Arch Histol Cytol 53:95–102

    Google Scholar 

  28. Young RW (1962) Cell proliferation and specialization during endochondral osteogenesis in young rats. J Cell Biol 14:357–370

    Google Scholar 

  29. Rasmussen H, Bordier P (1974) The physiological and cellular basis of metabolic bone disease. Williams and Wilkins Co., Baltimore, pp 35–70

    Google Scholar 

  30. Zambonin Zallone A, Teti A (1981) The osteoclasts of hen medullary bone under hypocalcaemic conditions. Anat Embryol 162:379–392

    Google Scholar 

  31. Adachi K, Chole RA, Yee J (1991) Indomethacin inhibition of middle ear bone resorption. Arch Otolaryngol Head Neck Surg 117:267–269

    Google Scholar 

  32. Arita K, Kato K, Tojo T, Nishino M (1989) Studies on factors causing tooth resorption. 1. Role of prostaglandins as a chemical mediator on resorption of deciduous teeth in rabbits. Jpn J Pedodont 27:621–628

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Charles Salt Research Centre, The Robert Jones and Agnes Hunt Orthopaedic Hospital, SY10 7AG, Oswestry, Shropshire, United Kingdom

    M. J. Marshall, I. Holt & M. W. J. Davie

Authors
  1. M. J. Marshall
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Holt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. W. J. Davie
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work has been presented in part as an abstract to the Bone and Tooth Society Summer meeting, July 1993

Rights and permissions

Reprints and permissions

About this article

Cite this article

Marshall, M.J., Holt, I. & Davie, M.W.J. The number of tartrate-resistant acid phosphatase-positive osteoclasts on neonatal mouse parietal bones is decreased when prostaglandin synthesis is inhibited and increased in response to prostaglandin E2, parathyroid hormone, and 1,25 dihydroxyvitamin D3 . Calcif Tissue Int 56, 240–245 (1995). https://doi.org/10.1007/BF00298618

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00298618

Key words

Search

Navigation