Skip to main content
SpringerLink
Log in

A tetrazolium method for non-specific alkaline phosphatase

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

A technique for the histochemical demonstration of non-specific alkaline phosphatase was developed using a medium containing indoxyl phosphate and a tetrazolium salt, Nitro B.T. The tetrazolium salt was reduced to diformazan by the hydrogen ions released by the formation of either indigo or indigo white by reaction of the enzyme on the indoxyl phosphate.

The localization in the organs investigated was similar to that obtained by the standard azo dye and lead techniques.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barrnett, R. J., Seligman, A. M.: Histochemical demonstrations of esterases by production of indigo. Science 114, 579–582 (1951).

    Google Scholar 

  • Gomori, G.: Distribution of acid phosphatase in tissues under normal and pathogenic conditions. Arch. Path. 32, 189–199 (1941).

    Google Scholar 

  • Holt, S. J.: A new approach to the cytochemical localization of enzymes. Proc. roy. Soc. B 142, 160–169 (1954).

    Google Scholar 

  • —: The value of fundamental studies of staining reactions to indoxyl methods for esterases. J. Histochem. Cytochem. 4, 541–554 (1956).

    Google Scholar 

  • MacGadey, J.: Modified indoxyl acetate technique for the histochemical demonstration of non-specific esterases in mouse testis. J. med. Lab. Technol. 24, 126–128 (1967).

    Google Scholar 

  • Seligman, A. M., Heymann, H., Barrnett, R. J.: The histochemical demonstration of alkaline phosphatase activity with indoxyl phosphate. J. Histochem. Cytochem. 2, 441–442 (1954).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, The University, Glasgow, Scotland

    James McGadey

Authors
  1. James McGadey
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

McGadey, J. A tetrazolium method for non-specific alkaline phosphatase. Histochemie 23, 180–184 (1970). https://doi.org/10.1007/BF00305851

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00305851

Keywords

Search

Navigation