Summary
Separate antisera were raised to the N- and C-terminal half of the diuretic hormone from Manduca sexta. Antisera against the two halves of this peptide recognized the same cells in M. sexta, and preabsorption of the antisera with the peptides used as antigens abolished the immunoreactivity, confirming their specificity. The antisera reacted with two median neurosecretory cells on each side of the protocerebral groove in larvae, and with a group of about 80 small median neurosecretory cells in the adult, as well as their axons to, and their axon terminals in, the corpora cardiaca. During the early pupal stages, small cells, which are possibly derived from a common neuroblast, differentiate into immunoreactive neurosecretory cells, which explains the large increase in cell numbers in the adult. In the sleepy sulphur butterfly, Eurema nicippe, homologous median neurosecretory cells in the adult were immunoreactive with both antisera.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agui N, Granger NA, Gilbert LI, Bollenbacher WE (1979) Cellular localization of the insect prothoracicotropic hormone: in vitro assay of a single neurosecretory cell. Proc Natl Acad Sci USA 76:5694–5698
Boer HH, Schot LPC, Roubos EW, Maat A ter, Lodder JC, Reichelt D, Swaab DF (1979) ACTH-like immunoreactivity in two electronically coupled giant neurons in the pond snail, Lymnaea stagnalis. Cell Tissue Res 202:231–240
Buys CM, Gibbs D (1981) The anatomy of neurons projecting to the corpus cardiacum from the larval brain of the tobacco hornworm, Manduca sexta. Cell Tissue Res 215:505–513
Copenhaver PF, Truman JW (1986) Metamorphosis of the cerebral neuroendocrine system in the moth, Manduca sexta. J Comp Neurol 249:186–209
El-Salhy M, Falkmer S, Kramer KJ, Speirs RD (1983) Immunohistochemical investigations of neuropeptides in the brain, corpora cardiaca, and corpora allata of an adult lepidopteran insect, Manduca sexta. Cell Tissue Res 232:295–317
Hinks CF (1971) A comparative study of the neurosecretory cells occurring in the adult brain of several species of Lepidoptera. J Entomol A 46:13–26
Homberg U, Davis NT, Hildebrand JG (1991) Peptide-immunocytochemistry of neurosecretory cells in the brain and retrocerebral complex of the sphinx moth, Manduca sexta. J Comp Neurol 303:35–52
Horodyski F, Riddiford LM, Truman JW (1989) Isolation and expression of the eclosion hormone gene from the tobacco hornworm, Manduca sexta. Proc Natl Acad Sci USA 86:8123–8127
Kataoka H, Troetschler RG, Kramer SJ, Cesarin BJ, Schooley DA (1987) Isolation and primary structure of the eclosion hormone of the tobacco hornworm, Manduca sexta. Biochem Biophys Res Commun 146:746–750
Kataoka H, Toschi A, Li JP, Carney RL, Schooley DA, Kramer SJ (1989a) Identification of an allatotropin from adult Manduca sexta. Science 243:1481–1483
Katoaka H, Troetschler RG, Li JP, Kramer SJ, Carney RL, Schooley DA (1989b) Isolation and identification of a diuretic hormone from the tobacco hornworm, Manduca sexta. Proc Natl Acad Sci USA 86:2976–2980
Marti T, Takio K, Walsh KA, Terzi G, Truman JW (1987) Microanalysis of the amino acid sequence of the eclosion hormone from the tobacco hornworm, Manduca sexta. FEBS Lett 219:415–418
Morgan PJ, Mordue W (1984) Diuretic hormone: another peptide with widespread distribution within the insect CNS. Physiol Entomol 9:197–206
Nijhout HF (1975) Axonal pathways in the brain-retrocerebral neuroendocrine complex of Manduca sextd. Int J Insect Morphol Embryol 4:529–538
O'Brien MA, Katahira EJ, Flanagan TR, Arnold LW, Haughton G, Bollenbacher WE (1988) A monoclonal antibody to the insect prothoracicotropic hormone. J Neurosci 8:3247–3257
Panov AA (1983) The median neurosecretory cells in the brain of Lepidoptera. Zool Anz 210:180–203
Ponov AA, Kind TW (1963) The system of the neurosecretory cells in the brain of Lepidoptera. Dokl Akad Nauk SSSR 153:1186–1189
Petzel DH, Hagedorn HH, Beyenbach KW (1985) Preliminary isolation of mosquito natriuretic factor. Am J Physiol 249:R379-R386
Proux JP, Miller CA, Li JP, Carney RL, Girardie A, Dellage M, Schooley DA (1987) Identification of an arginine vasopressin-like diuretic hormone from Locusta migratoria. Biochem Biophys Res Commun 149:180–186
Raabe M (1982) Insect neurohormones. Plenum, New York London
Raabe M (1983) The neurosecretory-neurohaemal system of insects: anatomical, structural and physiological data. Adv Insect Physiol 17:205–303
Raabe M (1989) Recent developments in insect neurohormones. Plenum, New York London
Rémy C, Girardie J (1980) Anatomical organization of two vasopressin-neurophysin-like neurosecretory cells throughout the central nervous system of the migratory locust. Gen Comp Endocrinol 37:93–100
Sanes JR, Hildebrand JG (1976) Structure and development of antennae in a moth, Manduca sexta. Dev Biol 51:282–299
Schooneveld H, Romberg-Privee HM, Veenstra JA (1987) Phylogenetic differentiation of glandular cells in corpora cardiaca as studied immunocytochemically with region-specific antisera to adipokinetic hormone. J Insect Physiol 33:167–176
Skowsky WR, Fisher DA (1972) The use of thyroglobulin to induce antigenicity to small molecules. J Lab Clin Med 80:134–144
Spring JH (1990) Endocrine regulation of diuresis in insects. J Insect Physiol 36:13–22
Sternberger LA (1974) Immunocytochemistry. Wiley, New York
Swaab DF, Pool CW, Leeuwen F van (1977) Can specificity ever be proved in immunocytochemical staining? J Histochem Cytochem 25:388–391
Tager HS (1975) Coupling of peptides to albumin with difluorobenzene. Anal Biochem 71:367–375
Taghert PH, Truman JW (1982) Identification of the bursicon containing neurones in abdominal ganglia of the tobacco hornworm, Manduca sexta. J Exp Biol 98:385–401
Truman JW, Copenhaver PF (1989) The larval eclosion hormone neurones in Manduca sexta: identification of the brain-proctodeal neurosecretory system. J Exp Biol 147:457–470
Tublitz NJ, Truman JW (1985) Identification of neurones containing cardio-acceleratory peptides (CAPs) in the ventral nerve cord of the tobacco hawkmoth, Manduca sexta. J Exp Biol 116:395–410
Veenstra JA (1988) Immunocytochemical demonstration of vertebrate peptides in invertebrates: the homology concept. Neuropeptides 12:49–54
Veenstra JA, Schooneveld H (1984) Immunocytochemical localization of neurons in the nervous system of the Colorado potato beetle with antisera against FMR Famide and bovine pancreatic polypeptide. Cell Tissue Res 235:303–308
Veenstra JA, Romberg-Privee HM, Schooneveld H, Polak JM (1985) Immunocytochemical localization of peptidergic neurons and neurosecretory cells in the neuro-endocrine system of the Colorado potato beetle with antisera to vertebrate regulatory peptides. Histochemistry 82:9–18
Wheelock GD, Petzel DH, Gillett JD, Beyenbach KW, Hagedorn HH (1988) Evidence for hormonal control of diuresis after a blood meal in the mosquito, Aedes aegypti. Arch Insect Biochem Physiol 7:75–89
Ziegler R, Hoff R, Rohde M (1988) Storage site of glycogen phosphorylase activating hormone in larvae of Manduca sexta. J Insect Physiol 34:143–150
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Veenstra, J.A., Hagedorn, H.H. Identification of neuroendocrine cells producing a diuretic hormone in the tobacco hornworm moth, Manduca sexta . Cell Tissue Res 266, 359–364 (1991). https://doi.org/10.1007/BF00318191
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00318191