Abstract
Guanine is determined at the 5.0×10−10 −2.0×10−7 mol/l level by differential-pulse adsorptive stripping voltammetry at a hanging mercury drop electrode using the reduction peak of its copper (II) complex at −0.21 V vs. Ag-AgCl electrode. The optimum analytical conditions were found to be Britton-Robinson buffer solution (pH 4.8), an accumulation potential of 0.0 V and an accumulation time of 3 min. Under these conditions, the detection limit is 5.0×10−10 mol/l and the relative standard deviation 2.6% for 1.0×10−7 mol/l guanine. The method is compared with the previous voltammetric methods. The presence of some purine derivatives does not interfere.
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Shubietah, R.M., Abu Zuhri, A.Z. & Fogg, A.G. Determination of nanomolar levels of guanine by differential-pulse adsorptive cathodic stripping voltammetry of its copper(II) complex. Fresenius J Anal Chem 348, 754–757 (1994). https://doi.org/10.1007/BF00323699
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DOI: https://doi.org/10.1007/BF00323699