Abstract
A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-acid residues. Comparison with other fungal glucoamylase amino-acid sequences showed homologies of 37–48%. The glucoamylase cDNA, when introduced into Saccharomyces cerevisiae under the control of the yeast ADC1 promoter, directed the secretion of active glucoamylase P into the growth medium.
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Communicated by C. P. Hollenberg
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Vainio, A.E.I., Torkkeli, H.T., Tuusa, T. et al. Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae . Curr Genet 24, 38–44 (1993). https://doi.org/10.1007/BF00324663
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DOI: https://doi.org/10.1007/BF00324663