Summary
Flavonoid biosynthetic enzymes are known to be coordinately induced in several plants. Evidence is presented here for the existence of regulatory genes in maize that govern the joint expression of unlinked structural genes specifying two flavonoid biosynthetic enzymes in the aleurone layer of the seed. UDPglucose 3-O-glucosyltransferase (UFGT) is specified by the Bz locus in chromosome 9, whereas chalcone synthase (CHS) seems to be encoded by the C2 locus in chromosome 4. Mutations at these respective loci cause the elimination of either UFGT or CHS, exclusively. Mutations at several other loci (C, R, Vp, Clf) affect both enzymes simultaneously. A functional allele at each of the latter loci is required for the coordinate expression of CHS, UFGT, and in all likelihood, other flavonoid biosynthetic enzymes.
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References
Chen SM, Coe EH (1977) Control of anthocyanin synthesis by the C locus in maize. Biochem Genet 15:333–346
Coe EH (1955) Anthocyanin synthesis in maize: the interaction of A2 and Pr in leucoanthocyanin accumulation. Genetics 40:568
Dooner HK (1979) Flavonol glucosyltransferase activity in bronze embryos of Zea mays. Phytochemistry 18:749–751
Dooner HK (1980a) gay, a new mutant in chromosome 1. Maize Genet Coop Newsl 54:79–80
Dooner HK (1980b) Genetic regulation of anthocyanin biosynthetic enzymes in maize. Genetics 94:29
Dooner HK (1981) Regulation of the enzyme UFGT by the controlling element Ds in bz-m4, an unstable mutant in maize. Cold Spring Harbor Symp Quant Biol 45:457–462
Dooner HK, Kermicle JL (1971) Structure of the R-r tandem duplication in maize. Genetics 67:427–436
Dooner HK, Nelson OE (1977a) Genetic control of UDPglucose-flavonol 3-O-glucosyltransferase in the endosperm of maize. Biochem Genet 15:509–515
Dooner HK, Nelson OE (1977b) Controlling element-induced alterations in UDPG: flavonoid glucosyltransferase, the enzyme specified by the bronze locus in maize. Proc Natl Acad Sci USA 74:5623–5627
Dooner HK, Nelson OE (1979) Interaction among C, R and Vp in the control of the Bz glucosyltransferase during endosperm development in maize. Genetics 91:309–315
Greenblatt IM (1975) Linkage relations of the diffuse factor on chromosome 4. Maize Genet Coop Newsl 49:23–25
Grisebach H, Hahlbrock K (1974) Enzymology and regulation of flavonoid and lignin biosynthesis in plants and plant cell suspension culture. Recent Adv Phytochem 8:21–52
Gross GG, Zenk MH (1966) Darstellung und Eigenschaften von Coenzym A-Thiolestern substituierten Zimtsauren. Z Naturforschg 21b:683–690
Hahlbrock K (1981) Messenger RNA induction as a prerequisite for flavonoid biosynthesis in parsley. Hoppe-Seyler's Z Physiol Chem 362:8–9
Hahlbrock K, Ebel L, Ortmann R, Sutter A, Wellmann E, Grisebach H (1971) Regulation of enzyme activities related to the biosynthesis of flavone glycosides in cell suspension cultures of parsley. Biochim Biophys Acta 224:7–15
Heller W, Hahlbrock K (1980) Highly purified “flavanone synthase” from parsley catalyzes the formation of naringenin chalcone. Arch Biochem Biophys 200:617–619
Kermicle JL (1970) Dependence of the R-mottled phenotype in maize on mode of sexual trasmission. Genetics 66:69–85
Kirby LY, Styles ED (1970) Flavonoids associated with specific gene action in maize and the role of light in substituting for the action of a gene. Can J Genet Cytol 12:934–940
Kreuzaler F, Hahlbrock K (1975) Enzymic synthesis of an aromatic ring from acetate units. Eur J Biochem 56:205–213
Kreuzaler F, Ragg H, Heller W, Tesch R, Witt I, Hammer D, Hahlbrock K (1979) Flavanone synthase from Petroselinum hortense. Eur J Biochem 99:89–96
Kuhn B, Forkmann G, Seyffert W (1978) Genetic control of chalcone-flavanone isomerase activity in Callistephus chinensis. Planta 138:199–303
Larson R, Coe EH (1968) Enzymatic action of the Bz anthocyanin factor on maize. Proc XII Int Congr Genet 1:131
Larson R, Coe EH (1977) Gene-dependent flavonoid glucosyl-transferase in maize. Biochem Genet 15:153–156
McCormick S (1978) Pigment synthesis in maize aleurone from precursors fed to anthocyanin mutants. Biochem Genet 16:777–786
Neuffer GM (1978) Induction of genetic variability. In: Walden DB (ed) Maize breeding and genetics. John Wiley, NY, pp 579–600
Potty VH (1969) Determination of protein in the presence of phenols and pectins. Anal Biochem 29:535–539
Reddy AR, Reddy GM (1971) Chemicogenetic studies of leucoanthocyanidin in a maize mutant. Curr Sci 40:335–337
Reddy GM, Coe EH (1962) Inter-tissue complementation: A simple technique for direct analysis of gene-action sequence. Science 138:149–150
Reddy GM, Reddy AR (1975) Gene action in the synthesis of flavonoid pigments in aleurone tissue of maize. Int Maize Symp Genet Breeding Abstr:30
Schroder J, Heller W, Hahlbrock K (1979) Flavanone synthase: simple and rapid assay for the key enzyme of flavonoid biosynthesis. Plant Sci Lett 14:281–286
Soave C, Suman N, Viotti A, Salamini F (1978) Liukage relationships between regulatory and structural gene loci involved in zein synthesis in maize. Theor Appl Genet 52:263–267
Soave C, Tardani L, DiFonzo N, Salamini F (1981) Zein level in maize endosperm depends on a protein under control of the opaque-2 and opaque-6 loci. Cell 27:403–410
Spribille R, Forkmann G (1981) Genetic control of chalcone synthase activity in flowers of Matthiola incana R. Br. Z Naturforsch 36c:619–624
Styles ED, Ceska O (1972) Flavonoid pigments in genetic strains of maize. Phytochemistry 11:3019–3021
Styles ED, Ceska O (1977) The genetic control of flavonoid synthesis in maize. Can J Genet Cytol 19:289–302
Sutfield R, Wiermann R (1980) Chalcone synthesis with extracts from tulip anther tapetum using a biphasic enzyme assay. Arch Biochem Biophys 201:64–72
Sutfield R, Wiermann R (1981) Purification of chalcone synthase from tulip anthers and comparison with the synthase from Cosmos petals. Z Naturforsch 36c:30–34
Sutter A, Grisebach H (1973) UDPglucose flavonol 3-O-glucosyl-transferase from cell suspension cultures of parsley. Biochim Biophys Acta 309:289–295
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Dooner, H.K. Coordinate genetic regulation of flavonoid biosynthetic enzymes in maize. Molec Gen Genet 189, 136–141 (1983). https://doi.org/10.1007/BF00326066
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DOI: https://doi.org/10.1007/BF00326066